... cultured in medium containing 5 m
M
glucose,
transfected with the ovine SGLT1 promoter fragment, and
then either maintained in the same medium or transferred to
one containing 25 m
M
glucose, in the ... sensing (Eur. J. Biochem. 270) 3383
that there is an increase in intracellular cAMP levels in
response to increasing levels of medium
D
-glucose.
Involvement of a GPCR...
... recovered,
only the longest amino acid sequences that included
the DNA binding, hinge and ligand-binding domains
were used for this analysis, except for the Knirps fam-
ily members that lack the LBD. In D. ... reorganization of the follicular epithelium
in the resulting adults [40]. DmE75A and DmE75B
have been implicated in defining the transition stages
8 and 9 of the...
... interpretation in distinguish-
ing interacting from noninteracting proteins. One
Fig. 1. Schematics of library designs. The helical wheel diagram looks down the axis from the N-terminus to the C-terminus. ... focused on small molecule inhibitors, but
the potential of using peptides as the starting point in
the generation of therapeutics is now a growing area
[18,19]. Peptide...
... suggesting their complete cellular
segregation from A-type lamins when the NE is disas-
sembled [10a]. In mammalian cells lamins bind in vitro
to many known INM proteins, including emerin,
MAN1, ... complexes
by the NE proteins as illustrated in Fig. 1. By serving
as docking sites at the INM, LAP2b, LBR, and poss-
ibly other INM proteins, can anchor DNA, chromatin
and chromatin mo...
... lead to the complete mineraliza-
tion of the pyrrolidine ring of nicotine, which after
oxidation by 6-hydroxy-l-nicotine oxidase, is cleaved
off from the pyridine ring of nicotine in the form ... enzymes
involved in nicotine catabolism will have applications
not only in the bioremediation of nicotine waste, but
also in the supply of nicotine derivatives as starting
materia...
... computing the G + C
content in sliding windows moving along the genome.
Fig. 4. The negative cumulative GC profile
for GGA12 marked with the segmentation
points obtained. The bottom five plots show
the ... all the seg-
ments obtained at t
0
¼ 100 without the constraint of the minimum
length in the draft genome sequence of chicken. (A) The length dis-
tribution of all...
... in epileptic patients [27]. Other mechanisms may
be associated with the increase in ROS levels in the
epilepsy model as well as in neurodegeneration
observed in epileptic humans [18,28].
During ... findings show that pilocarpine induces SE,
which can produce alterations in superoxide dismutase
and catalase activities in different areas, thereby pro-
tecting the brain from n...
... productive binding of steroid substrates:
in the first, a (small) sub population of enzyme binds
directly the steroid in the reactive conformation, with
the remainder of the enzyme binding the unreactive
conformation. ... unusual in having
two histidine residues (rather than the His-Asn pair
seen in OYE, MR and some of the other member
proteins; Fig. 1B) in the acti...
... lCiÆmL
)1
[
3
H]inositol for 24 h in inositol-free medium
containing 10% (v ⁄ v) dialyzed fetal bovine serum. Cells
were then washed and incubated in NaCl ⁄ P
i
containing
20 mm Li
+
with the agonist ... RGS domain in the amino-terminus of G
protein-coupled receptor kinase 2 inhibits Gq-mediated
signaling. Int J Mol Med 5, 335–340.
20 Chen CA & Manning DR (2001) Regulation of G...
... determined by incubating the diluted recombin-
ant proteins in the standard reaction mixture in the presence
of increasing concentrations of PP
i
(0.05–0.5 m
M
)at55°C.
Data were fit to the appropriate ... rate was then calculated.
Larval molting inhibition assay
To confirm whether the native PPase enzyme is involved in
the molting process, we examined the effects of two PPa...