... Physicochemical characterization and biological activity
of a glycoglycerolipid from
Mycoplasma fermentans
Klaus Brandenburg
1
, Frauke Wagner
1
, Mareike Mu¨ ller
1
, Holger ... Chicester.
32. Mu
¨
hlradt, P.F. & Frisch, M. (1994) Purification and partial bio-
chemical characterization of a Mycoplasma fermentans- derived
substance that activates macrophage...
... enzymatic characterization of
HpSDH demonstrates its activity with k
cat
of 7.7 s
)1
and K
m
of 0.148 mm
toward shikimate, k
cat
of 7.1 s
)1
and K
m
of 0.182 mm toward NADP, k
cat
of 5.2 s
)1
and ... determined by Bradford assay using bovine serum
albumin as standard.
Enzymatic activity assay
The enzymatic activity of HpSDH was assayed at 25 °Cby
monitoring the red...
... some sera
to a 9- and a 15-kDa band. We could show that the
9-kDa band in the tomato extracts reacts with a specific
antibody against the LTP from cherry, Pru av 3 and the
15-kDa band shows reactivity ... sequence of the coding region: 5¢TTAC
AAGGACAAATTAATTGTGCCAG. For amplification
of the long isoform the same 5¢ primers were used, the 3¢
specific primer was FF3B: 5¢TTACAAGTCTT...
... Teruna J. Siahaan
3
and Seetharama D. S. Jois
1
1
Department of Pharmacy and
2
Department of Microbiology, National University of Singapore, Singapore;
3
Department of
Pharmaceutical Chemistry, ... possible pair of structures,
and was divided into several conformational families. The
average structure was taken from each family and chosen
as starting structures for the cal...
... ATCCTCACGAACAAGCAG
5RACR GATCGCGATGCAGGCCTT
FLF1 GGACGACTACAGCGTCTTCAGTAGA
FLR1 TCCAAACAGTCAGTTTCTTAACCGT
Table 1. Purification of cellulase from abalone Haliotis discus hannai. Oneunitofcellulasewasdefinedastheamountofenzymethatliberates
reducing ... Double-stranded cDNA was syn-
thesized from the mRNA with a cDNA synthesis kit
(TaKaRa, Tokyo, Japan) and used as an abalone cDNA
libra...
... doi:10.1046/j.1432-1033.2002.03108.x
Several intermolecular dioxygenases, particularly those of
microbial or human origin, catalyze reactions of medicinal
and industrial relevance, and their spatial organization and
mode of action are ... Ipomea nil and Medicago sativa), three anthocyanidin synthases (Zea mays, Anthirrhinum majus and Oryza sativa), five gibberellin
C20 oxidases...
... as a substrate, and the
absorbance was measured at 490 nm by using a Spectramax
plate reader and software (Molecular Devices, Sunnyvale,
CA, USA). All values were interpolated from either a log-
log ... the
average n molÆmg
)1
with standard deviations (±) obtained from
quantifying and averaging areas under specific peaks from GLC
analysis of four separate samples of LGL
B...
... established, on the basis
of morphological changes, reduction of mitochondrial
membrane potential, increases in cytoplasmic cyto-
chrome c and Smac ⁄ DIABLO, and caspase-3 and
caspase-9 activation. ... MINIREVIEW
Marine toxins and the cytoskeleton: pectenotoxins,
unusual macrolides that disrupt actin
Begon
˜
a Espin
˜
a
1
and Juan A. Rubiolo
1,2
1 Departamento de Farmacolog...
... factor 1 homeodomain – hints from
15
N-NMR
relaxation studies
Devrim Gu
¨
mral, Luana Nadalin, Alessandra Corazza, Federico Fogolari, Giuseppe Damante,
Paolo Viglino and Gennaro Esposito
Dipartimento ... experimental R
1
and R
2
data was assessed by measurement duplication over a series
of arbitrarily selected relaxation delays (at least three;
see supplementary Doc. S1). The averag...