Tài liệu Báo cáo khoa học: Adaptive changes in the expression of nuclear and mitochondrial encoded subunits of cytochrome c oxidase and the catalytic activity during hypoxia pptx
... Adaptive changes in the expression of nuclear and mitochondrial
encoded subunits of cytochrome
c
oxidase and the catalytic
activity during hypoxia
C. Vijayasarathy
1,
*
,†
, ... though the catalytic efficiency
of the enzyme (TN for cytochrome c oxidase) remained
nearly the same. Increased glycolytic flux and alterations in
the kinet...
... toxin
is cleaved by furin into two fragments: the A chain
corresponding to the catalytic domain (C domain);
and the B chain corresponding to the translocation
domain (T domain) and the receptor-binding ... The acidic pH in the endo-
some triggers a conformational change, leading to
insertion of the toxin in the membrane. The C domain
is then translocated acr...
... a training lex-
icon consisting of correct word pairs. The initial
transducer contains uniform probabilities. It is used
to transduce the word pairs of the training lexicon,
thereby counting all ... that these two tech-
niques can be combined efficiently. They use Rapp’s
co-occurrence vectors in combination with Mann
and Yarowsky’s EM-trained transducer.
3 Two-Stage Models of L...
... yields
three fractions which are denoted according to the proteins
they contain. Fraction 1 includes all ‘non -nuclear proteins’,
i.e. cytosolic proteins separated during hypotonic prepar-
ation of nuclei, ... doi:10.1046/j.1432-1033.2003.03769.x
functional complex, indicating a speci c in uence of
O
2
-dependent cellular changes on critical steps of the
assembly of a funct...
... for
that description (e.g., about the monument’s ar-
chitect) are dynamically included in the rules of
the speech recognizer’s grammar, to increase word
recognition accuracy. The rules include compo-
nents ... generated descriptions vary dynamically, in
both content and language expressions, depending
on the interaction profile as well as the dynamic
interaction history. T...
... for introduction of unmarked
mutations in the genome of Paracoccus denitrificans –
construction of strains with multiple mutations in the
genes encoding periplasmic cytochrome- C
550
, cyto-
chrome -C
551
, ... cytochrome
cd
1
, is necessary for the biogenesis of the d
1
cofactor
[9–13]. In P. denitrificans and closely related Para-
coccus pantotrophus, these genes...
... Cell-cycle regulation is important in growth
control, and therefore deregulation of the cell-cycle
machinery has been implicated in carcinogenesis [57].
Cyclins and cyclin-dependent kinases (CDKs), ... up-regulation of CDK inhib-
itors, including KIP family members and
INK4 family members Members of the KIP
family can inhibit the catalytic activity of
CDK2, 4 and...
... obtained by PCR from the
plasmid pET21 ⁄ SIC1 [32] with a forward primer (5¢-TAC
CTGGCCAATGAATATGCATCATCATCATCATCATA
CTCCGTCGACCCCACC-3¢) designed to introduce a
hexahistidine tag and a ClaI ... (FWN
1
:5¢-TACCG
TTAACATCGATATGCATCATCATCATCATCATAC-3¢),
designed to introduce a ClaI restriction site at nucleotide
position –6 and a reverse primer (REVN
1
:5¢-CCTGCC
ATTGCTTGCAGCC-3¢) that in...
... oligonucleotide (GL70, 5¢-bio-
tin-CTC CAG TGA ATC CCA GAA GAC TCT GGA
G-3¢; GL70mt, 5¢-biotin-CTC CAG TAG ATC TCA AGA
GAC TCT GGA G-3¢) in binding buffer [50 mm Tris ⁄ HCl,
pH 7.8, 100 mm NaCl, ... )272 and )13 (forward, 5¢-CCA TGG AGA
CCA ACA CCC T-3¢; reverse, 5¢-CCC TGG GCT TTT
ATA AGT CGT-3¢), or the human hsp70 gene between
)1860 and ) 656 (forward, 5¢-TCT ATC TCT CGA TGG
ATA CA...
... respectively. The lines in each graph (E, F) indicate the
amount of binding predicted if the wild-type and mutant receptors
are binding ligand independently. The tables indicate the amounts
of the ... at 4 C. The lines in each graph (F, G) indicate the
amount of binding predicted if the wild-type and mutant receptors
are binding ligand independently. The tabl...