Tài liệu Báo cáo khoa học: The tungsten-containing formate d

Tài liệu Báo cáo khoa học: The tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1: Puriﬁcation and properties docx

... The tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1: Puriﬁcation and properties Markus Laukel 1,2 , Ludmila Chistoserdova 3 , Mary E. Lidstrom 3,4 and Julia ... time, puriﬁcation and properties of a tungsten-containing NAD-dependent FDH from the aero- bic bacterium, M. extorquens AM1. Materials and methods Organism and g...

Ngày tải lên : 20/02/2014, 23:20

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Tài liệu Báo cáo khoa học: The alr2505 (osiS) gene from Anabaena sp. strain PCC7120 encodes a cysteine desulfurase induced by oxidative stress docx

... 3715 the sulfur atom is released from the persulﬁde and the active site of the enzyme (i.e. the cysteine residue) becomes accessible [17]. In vitro, this step can be achieved by decomposing the ... that of IscS (Fig. 1A), with a two-domain organization and the presence of both a-helices and b-strands. On the basis of these data and the fact that alr2505-expression...

Ngày tải lên : 18/02/2014, 04:20

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Tài liệu Báo cáo khoa học: The resident endoplasmic reticulum protein, BAP31, associates with c-actin and myosin B heavy chain Analysis by capillary liquid chromatography microelectrospray tandem MS ppt

... associations with newly synthesized IgD and cellubrevin as they exit from the ER to the Golgi apparatus [5] and it has been recently shown to participate in the quality control of the cystic ﬁbrosis ... in the BAP31 immunopre- cipitate, indicate that the association between BAP31 and c-actin was the result of speciﬁc interactions. Discussion We describe in this paper th...

Ngày tải lên : 20/02/2014, 23:20

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Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efﬁciency doc

... reported concerning the functions and relationship of the dual domains. Here we describe the physical properties that relate to the catalysis of a newly isolated, dual-domain BPP (PhyH) from Bacillus sp. ... pH (6.0–8.0) and at low temperatures (0–35 °C). We focus on the function and relationship of the two single domains. Additionally, the possibility that fusion of...

Ngày tải lên : 14/02/2014, 15:20

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Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

... positioned these kinases as new downstream OXI1 components and named the proteins PTI1-1, 1-2 and 1-3. One of the prey cDNA encoded PTI1-1 (At1g06700) and a second prey cDNA encoded another member of the ... indicated time points. The mixes were then incubated with [c- 32 P]-ATP and MBP (10 l g) for 30 min. The top panel shows the kinase assay visualized by autoradiography...

Ngày tải lên : 14/02/2014, 19:20

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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

... together with the E2F site [20,21]. The DRS ⁄ CHR in the B-Myb promoter deviates most from other CHR sequences with its two-nucleotide exchange from the CHR consensus (Fig. 2). Changing the ... with the well-characterized CDE ⁄ CHR sites from the human Cdc25C and cyclin A promoters [10] and expression from the altered reporters was tested during the cell cycle...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt

... through the mitochondrial pathway. The key event in the mitochondrial pathway is the release of proapoptotic factors from the mitochondrial intermembrane space into the cytosol, resulting in the ... Maintaining the balance between cell survival and apoptosis is critical in the maintenance of a healthy organism, and tipping the equilibrium in one or another direct...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: The plasminogen activator inhibitor 2 transcript is destabilized via a multi-component 3¢ UTR localized adenylate and uridylate-rich instability element in an analogous manner to cytokines and oncogenes pdf

... of the ARE I-AUUUA and ARE III-AUUUA sequences used primers SJS172 and SJS173, and SJS174 and SJS175, respectively. Mutation of the atypical AU-rich sequence used primers SJS259 and SJS260, and ... SJS260, and the creation of the ARE II ⁄ ARE IV double mutant used primers SJS261 and SJS262. The mutagenesis of ARE I and III introduced HindIII restriction sites and...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc

... between the nucleus and the cytoplasm. Genes Dev 12, 55–66. 31 Gui JF, Tronche ` re H, Chandler SD & Fu XD (1994) Puriﬁcation and characterization of a kinase speciﬁc for the serine- and arginine-rich ... in the UTRs of the transcript and the trans-acting factors (RNA-binding proteins and noncoding regulatory RNAs) that bind to these sequences. Among the best stu...

Ngày tải lên : 16/02/2014, 15:20

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Tài liệu Báo cáo khoa học: The two Caenorhabditis elegans metallothioneins (CeMT-1 and CeMT-2) discriminate between essential zinc and toxic cadmium docx

... with thiol groups, and the cadmium speciation of the wild-type and the CeMT-2 knockout strain was distinctly different to the CeMT-1 and double knock- outs. Taken together, and supported by a simple ... to the total EXAFS spectra much smaller than the Cd–S contribution. Nevertheless, this conﬁrms that the cadmium coordina- tion environments of the CeMT-1 KO and doubl...

Ngày tải lên : 16/02/2014, 15:20

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