Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx

Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx

Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx

... butyrylcho- linesterase does not operate for catalysis at low pH. The mechanism explaining the catalytic behaviour of butyryl- cholinesterase at low pH in the presence of excess substrate remains to ... between bk cat and k cat at high pH is large relative to the difference at low pH, and the difference between bk cat at high pH and bk cat at...
Ngày tải lên : 20/02/2014, 23:20
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Tài liệu Báo cáo khoa học: High-affinity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

Tài liệu Báo cáo khoa học: High-affinity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

... that the I1572T mutation residing in the heart of the IGF-II binding domain in repeat 11 disrupts IGF-II binding to the receptor [22,31–33]. The ligand blotting data shown in Fig. 1C confirm that ... (B). The lines in each graph indicate the amount of binding predicted if the wild-type and mutant receptors are binding ligand independently. The triangles indicate a...
Ngày tải lên : 18/02/2014, 08:20
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Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

... state, which initi- ates the translocation of the catalytic domain. Here, the data allowed identification of the core of the pro- tein in the N state and the evolution of the overall structure of ... exchange The T domain was dissolved in 4 mm citrate-phosphate buf- fer, pH 7.0, at a concentration of 70 lm. One part of the solution was kept at pH 7...
Ngày tải lên : 16/02/2014, 09:20
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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

... anti- GFP-reacting 30 kDa band was not detected in the immunoprecipitates of antibody against calpain 7, indicating a lack of MIT domains in 30 K. Thus, mGFP–calpain 7 contains at least two cleavage ... lies at the N-terminal boundary of the protease domain, generating 45 K, and the other lies between mGFP and MITa, generating 30 K (Fig. 6A). To roughly estimate the la...
Ngày tải lên : 18/02/2014, 04:20
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx

Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx

... affinity copper binding protein, and (b) that amyloid fibril formation of this protein is diminished in presence of Cu 2+ ions. Another interesting finding is that copper binding gets less strong at pH 5, ... subtraction of data from a series of injections of copper chelate solution into a buffer blank correlating to the heat of dilution of the copper complex. After sub...
Ngày tải lên : 19/02/2014, 06:20
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Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt

Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt

... double- ringed BchI-BchD structure the ATPase activity of BchI is blocked. A conformational transition upon binding to BchH may bring the integrin I domain of BchD into contact with the integrin-binding ... integrin-binding motif of BchH, simultaneously triggering porphyrin metallation. This would also lead to a release of the blockade of the ATP-binding site of BchI b...
Ngày tải lên : 19/02/2014, 12:20
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Tài liệu Báo cáo khoa học: Structure-activity relationships of a-conotoxins targeting neuronal nicotinic acetylcholine receptors ppt

Tài liệu Báo cáo khoa học: Structure-activity relationships of a-conotoxins targeting neuronal nicotinic acetylcholine receptors ppt

... understanding the complexity involved in binding to the nAChR. A summary of this structural information and the insights into structure -activity relation- ships of a-conotoxins is presented in this ... attributed to the longer side chain of the leucine at position 10. In general, for the 4/7 a-conotoxins there appears to be a correlation between the length of...
Ngày tải lên : 19/02/2014, 12:20
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Tài liệu Báo cáo khoa học: KIPase activity is a novel caspase-like activity associated with cell proliferation doc

Tài liệu Báo cáo khoa học: KIPase activity is a novel caspase-like activity associated with cell proliferation doc

... KIPase activity in the indicated cell lines was assessed using the same assay. The data are expressed relative to the activity measured in the absence of z-VAD-fmk. In test assays z-VAD-fmk inhibited human ... the cells were incubated for 4 h at 37 °C (in triplicate). The cells were then harvested onto filters and the incorporation of labelled thymidine into...
Ngày tải lên : 19/02/2014, 13:20
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Tài liệu Báo cáo khoa học: Endotoxic activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L2 and Chlamydophila psittaci 6BC pdf

Tài liệu Báo cáo khoa học: Endotoxic activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L2 and Chlamydophila psittaci 6BC pdf

... [20]. At low LPS concentrations, blocking of CD14 completely abro- gated stimulation of human MNC. We investigated the remaining stimulatory activity of all LPS preparations after preincubation ... fragment ions of the de-O-acylated lipid A moiety around m/z 1119.75, and ions of minor intensity originating from the consecutive loss of three Kdo residues. Further ions...
Ngày tải lên : 20/02/2014, 23:20
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Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

... in loss of in vivo NirF activity. We demonstrate here that the product of the nirF gene is a periplasmic protein and, hence, must be involved in a late stage of the cofac- tor biosynthesis. In ... Alfred Phu ¨ ler at the University of Biele- feld are thanked for their kind gift of plasmid pMS255. Dr Katalin di Gleria at The Weatherall Institute of Molecular...
Ngày tải lên : 15/02/2014, 01:20
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