... butyrylcho-
linesterase does not operate for catalysis at low pH. The
mechanism explaining the catalytic behaviour of butyryl-
cholinesterase at low pH in the presence of excess substrate
remains to ... between bk
cat
and k
cat
at high pH is large relative to the difference at low pH, and
the difference between bk
cat
at high pH and bk
cat
at...
... that the I1572T mutation residing in the heart
of the IGF-II binding domain in repeat 11 disrupts
IGF-II binding to the receptor [22,31–33]. The ligand
blotting data shown in Fig. 1C confirm that ... (B). The lines in each graph indicate the amount of
binding predicted if the wild-type and mutant receptors are binding
ligand independently. The triangles indicate a...
... state, which initi-
ates the translocation of the catalytic domain. Here,
the data allowed identification of the core of the pro-
tein in the N state and the evolution of the overall
structure of ... exchange
The T domain was dissolved in 4 mm citrate-phosphate buf-
fer, pH 7.0, at a concentration of 70 lm. One part of the
solution was kept at pH 7...
... anti-
GFP-reacting 30 kDa band was not detected in the
immunoprecipitates of antibody against calpain 7,
indicating a lack of MIT domains in 30 K. Thus,
mGFP–calpain 7 contains at least two cleavage ... lies at the N-terminal boundary of the protease
domain, generating 45 K, and the other lies between
mGFP and MITa, generating 30 K (Fig. 6A). To
roughly estimate the la...
... affinity
copper binding protein, and (b) that amyloid fibril
formation of this protein is diminished in presence
of Cu
2+
ions. Another interesting finding is that
copper binding gets less strong at pH 5, ... subtraction of
data from a series of injections of copper chelate solution
into a buffer blank correlating to the heat of dilution of the
copper complex. After sub...
... double-
ringed BchI-BchD structure the ATPase activity of BchI is
blocked. A conformational transition upon binding to
BchH may bring the integrin I domain of BchD into contact
with the integrin-binding ... integrin-binding motif of BchH, simultaneously
triggering porphyrin metallation. This would also lead to a
release of the blockade of the ATP-binding site of BchI b...
... understanding the complexity involved in
binding to the nAChR. A summary of this structural
information and the insights into structure -activity relation-
ships of a-conotoxins is presented in this ... attributed to the longer
side chain of the leucine at position 10. In general, for the
4/7 a-conotoxins there appears to be a correlation between
the length of...
... KIPase activity in the indicated cell lines was assessed
using the same assay. The data are expressed relative to the activity measured in the absence of z-VAD-fmk. In test assays z-VAD-fmk inhibited
human ... the cells were incubated for
4 h at 37 °C (in triplicate). The cells were then harvested
onto filters and the incorporation of labelled thymidine into...
... [20]. At low
LPS concentrations, blocking of CD14 completely abro-
gated stimulation of human MNC. We investigated the
remaining stimulatory activity of all LPS preparations after
preincubation ... fragment
ions of the de-O-acylated lipid A moiety around m/z 1119.75,
and ions of minor intensity originating from the consecutive
loss of three Kdo residues. Further ions...
... in loss of in vivo
NirF activity. We demonstrate here that the product of the nirF gene is a
periplasmic protein and, hence, must be involved in a late stage of the cofac-
tor biosynthesis. In ... Alfred Phu
¨
ler at the University of Biele-
feld are thanked for their kind gift of plasmid pMS255.
Dr Katalin di Gleria at The Weatherall Institute of
Molecular...