... al. Proteasome degradation of G
q
proteins
Proteasome involvement in the degradation of the G
q
family of Ga subunits
Bente B. Johansson, Laura Minsaas and Anna M. Aragay
Department of Biomedicine, ... of Ga
16
and Ga
q
subunits. Therefore the lack of change in the rate of
degradation cannot be due to lack of receptor-activa-
tion of the...
... domain, a
transmembrane domain and an intracellular tyrosine
kinase domain. The binding of a ligand to the extracel-
lular domain of the EGFR induces receptor dimeriza-
tion, activation of the intracellular ... result in the up-regulation of CDK inhib-
itors, including KIP family members and
INK4 family members Members of the KIP
family can inhibit the catalyti...
... astrocytes, and
the tight junctions of vascular endothelial cells (ECs)
in the BBB are well organized with claudins and ZO-
proteins through a decrease in angiogenesis signaling
and an increase in the stability ... among
tissues in the body, and the risk of edema to brain
functions should be carefully considered. Brain edema
may increase pressure in the cranial cavit...
... [17–19].
Pantothenate kinase is an essential regulatory enzyme
in co-enzyme A biosynthesis, catalyzing the cytosolic
phosphorylation of pantothenate (vitamin B5),
N-pantothenoylcysteine and pantetheine ... destinations.
Cells from NPC subjects show a decrease in acid
sphingomyelinase activity, leading to the accumulation
of sphingomyelin [27]. Because one of the pathways
for c...
... amino acid biosynthesis. Much of
the generation of NADH during amino acid biosyn-
thesis takes place in the mitochondria. Because of the
block in the respiratory chain caused by the addition
of ... N
2
(containing less than 3 p.p.m. O
2
) at a flow
rate of 0.1 LÆmin
)1
per liter of medium, and a stir rate of
500 r.p.m. was maintained. In order to minimize the di...
... using the main chain of
residues Ser12 fiThr70 in t he structure a lignment. (B) O verlap of the
X-ray structure with the 30 °C NMR structure using the main chain of
residues Ser12fiThr70 in the ... effect: the binding of AFP
causes the ice between the bound proteins to grow as a
curved front, where further growth becomes energetically
unfavourable [7]. In this pro...
... it
joins the right bracket. The other rules are
identical to those for other domains, with the
group containing the pronominal part of the
relative pronoun having to join the other part
of the ... in the complementizer field.
In a sense, the complementizer field acts like
the fusion of the Vorfeld and the left bracket
of the main domain: The complementi...
... near
the N- and C-termini of the linkers: this suggests that
a mobile connection with both the catalytic domain
and the binding module is required for the function of
the nanomachine. Furthermore, ... spring, allowing
the nanomachine (catalytic domain–linker–binding
module) to crawl on the substrate surface. The possible
functional implications of the linker primary...
... Yer-
sinia, the increased synthesis of LcrV triggered by the
activation of the system leads to the titration of LcrG,
which binds LcrV in a 1 : 1 complex. In turn, this
results in a release of the ... by the sug-
gestion that pH sensing by Salmonella involves modi-
fications in the assembly of the translocon, which
affect the pH gradient within the needle,...
... 000 g for 10 min at 4 °C. The pellet, which contained
the mitochondria, was resuspended in 30 lL of Mito-Cyto
Buffer. The protein concentration was determined using the
bicinchoninic acid method ... sequencing. The
NdeI and BamHI restriction sites were used for cloning
the PCR fragments into the His-tag vector, pET-15b. The
recombinant proteins were generated in the E....