... were separated by SDS/
PAGE and stained by Coomassie brilliant blue. The two
hemocyanin bands were cut out and dispersed. About
100 lg of hemocyanin was used for the immunization of
rabbits. For ... submitted to MALDI-
TOF analysis and assigned to distinct subunits.
Fig. 1. SDS/PAGE and immunoblotting of S. coleoptrata hemocyanin.
About 10 lg of total hemolymph protein (HL...
... (1998), Grote et al.
(1997), Jayez and Rossari (1998) and Lagerwerf
(1998).
Secondly, the approach demands an understand-
ing of the
attentional characteristics
of presupposi-
tions. In particular, ... for NL understanding and/ or gener-
ation. But the work to date surely shows the benefit
of an approach that narrows the gap between dis-
course syntax and semantics and...
... Prevention of thermal inactivation and aggregation of lysozyme
by polyamines
Motonori Kudou
1
, Kentaro Shiraki
1
, Shinsuke Fujiwara
2
, Tadayuki Imanaka
3
and Masahiro Takagi
1
1
School of ... T
m
of lysozyme was 77.3 °C in the absence
of additives. The addition of 100 m
M
polyamines and Arg
slightly increased the T
m
of lysozyme by 1 °Cand0.5°C,...
... were
identified by the condensation and fragmentation of their
nuclei. The percentage of apoptotic cells was calculated as
the number of apoptotic cells divided by the total number
of cells counted. ... assembly and nucleocytoplasmic
transport of newly synthesized pre-rRNAs [11].
Because of its widespread distribution and broad range
of involvement, as well as the cross...
... embryogenesis and adulthood, and
functions by binding with two tyrosine kinase recep-
tors, VEGFR-1 (Flt-1) and VEGFR-2 (KDR ⁄ Flk-1),
Fig. 1. The VEGF–VEGFR system and the
inhibitors of various ... the Future’ of the Japan Society for the
Promotion of Science, and the program ‘Promotion of
Fundamental Research in Health Science’ of the Organi-
zation for Pharmaceutical...
... divergent
role and target genes of HIF-1a in different types of
cancer.
Malignant gliomas constitute a spectrum of brain
tumors with varying differentiation and malignancy
grades, and with clinical ... the
guidelines of the National Institutes of Health.
Tissue samples and immunohistochemistry
Tumor tissues from a total of 95 adult patients, 53 men
and 45 women (18 to 73...
... physiological role
of this interaction is conserved [30,52]. Interactions of
Vrp1p and Las17p in yeast and WIP and WASP-fam-
ily proteins in mammalian cells are direct and result
in constitutive and apparently ... localization of
Hof1p to the bud neck at elevated temperature and
this in turn is important for cytokinesis and cell
growth under these conditions.
Interestingly...
... proteolysis
by incorporation of a variety of ligands onto protease
SBL, including examples of natural PTMs such as
biotinylation and d-mannosylation (Fig. 3) [49]. The
pendant ligands allowed ... UK
Introduction
Post-translational modifications (PTMs) of proteins
modulate protein activity and greatly expand the diver-
sity and complexity of their biological function. The
ubiq...
... catabolic
and signaling) in different cells and tissues [3,7].
Several lines of evidence have suggested a key role
for iPLA
2
-VIA in control of the levels of phosphatidyl-
choline (PC) in cells by ... trend, peaking at G
1
and lowest at G
2
⁄ M and late
S phase. Reduction of U937 cell proliferation by inhibition of iPLA
2
-VIA
activity was associated with arrest in G
2...
... a
stator and a rotor. The stator is formed by subunits a
and b
2
, and the rotor is formed from multiple copies
of subunit c. They form an oligomeric ring of non-
covalently linked subunits, and rotation ... nFDl
Na
þ
where n is the number of translocated ions and F is
the Faraday constant.
The c subunit of the eukaryal V
1
V
0
ATPases present
in organelles arose by dupl...