... al. CD1d upregulation in iron- loaded HepG2 cells
FEBS Journal 272 (2005) 152–165 ª 2004 FEBS 165
Altered expression of CD1d molecules and lipid
accumulation in the human hepatoma cell line HepG2
after ... I molecules.
Herein, we report that parameters of iron metabolism were regulated in a
coordinated-fashion in a human hepatoma cell li...
... were picked and expanded. After
induction with doxycycline (1 lgÆmL
)1
), the individual
clones were tested for their expression of the protein of
interest.
Flag pull down
⁄
silver staining
⁄
mass ... observed, includ-
ing that Mxi1-SRa appears to be the predominant
transcript in the adult intestine and in the developing
embryo, whereas Mxi1-SRb transcripts predomina...
... nm,
comparable to the inhibition of the aminopeptidase
cathepsin H (K
i
¼ 30 nm ). In comparison with other
cystatins, cystatin F is a rather slow binding inhibitor
of the cathepsins, characterized ... activation of cystatin F we
have studied the in vitro kinetics of the interaction
between cystatin F and several cathepsins, as well
as their intracellular localization...
... [35] may not be considered in the light of the
results obtained in the present study. Rather, the
involvement of manganese in the in vivo assembly of
the metallocofactor of C. ammoniagenes R2F is ... ribonucle-
otide binding and radical transfer from the tyrosyl rad-
ical (Y122) of the R2 subunit to the cysteine (C439)
found in the active site of t...
... different kinetic and thermodynamic properties of the tetrameric enzyme
may result in fine-tuning of the properties of skeletal LDH-A, which is in line
with previously observed kinetic and thermodynamic ... located in the sub-
unit contact area of the tetramer, whereas the remaining two mismatches sur-
round the contact area; this is expected to manifest in t...
... short interfering RNA (siRNA) in human cells. Both
HO-2 mRNA and protein are expressed in the eight human cancer cell
lines examined, and HO-1 expression is detectable in five of the cell lines,
including ... analyzed the expression profiles of HO-1
and HO-2 in eight human cell lines by northern and
western blot analyses (Fig. 1A,B). Expression of H...
... [4–6]. In
order to improve the biological production method, a
detailed understanding of the biosynthesis of triterpene
saponins is required, including the enzymes catalyzing
the sequence of reactions ... reactions and the genes encoding these
enzymes.
The biosynthesis of triterpene saponins involves the
initial cyclization of 2,3-oxidosqualene, a common pre-
curso...
... (HUC-F cells) and WI-38 cells.
Expression of the human
Mcm2
gene
To understand the mechanism behind the enhanced
expression of Mcm proteins in HeLa cells, the concentra-
tions of Mcm2 mRNA in the ... Blue
staining.
Table 2. Quantitation and comparison of proteins in GM and WI-38
cells. From the data in Fig. 2 and others, the concentrations of Mcm...
... chain
corresponding to the catalytic domain (C domain);
and the B chain corresponding to the translocation
domain (T domain) and the receptor-binding domain.
The C and T domains remain covalently linked ... of the pro-
tein in the N state and the evolution of the overall
structure of the protein in the MG state. This degree
of resolution is unpreceden...
... mitochondria-targeting signal
in the yeast system.
Mitochondrial localization of intact CYP2E1
in yeast cells
Because of the existing ambiguity in the literature on
the nature and extent of CYP2E1 import into ... were initiated by the
addition of 3 mm NADPH, and incubation was continued
for 30 min at 37 °C in a shaking water bath. The reactions
were terminated...