Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... al. CD1d upregulation in iron- loaded HepG2 cells FEBS Journal 272 (2005) 152–165 ª 2004 FEBS 165 Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after ... I molecules. Herein, we report that parameters of iron metabolism were regulated in a coordinated-fashion in a human hepatoma cell li...
Ngày tải lên : 19/02/2014, 16:20
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Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt

Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt

... were picked and expanded. After induction with doxycycline (1 lgÆmL )1 ), the individual clones were tested for their expression of the protein of interest. Flag pull down ⁄ silver staining ⁄ mass ... observed, includ- ing that Mxi1-SRa appears to be the predominant transcript in the adult intestine and in the developing embryo, whereas Mxi1-SRb transcripts predomina...
Ngày tải lên : 18/02/2014, 16:20
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Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt

Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt

... nm, comparable to the inhibition of the aminopeptidase cathepsin H (K i ¼ 30 nm ). In comparison with other cystatins, cystatin F is a rather slow binding inhibitor of the cathepsins, characterized ... activation of cystatin F we have studied the in vitro kinetics of the interaction between cystatin F and several cathepsins, as well as their intracellular localization...
Ngày tải lên : 19/02/2014, 17:20
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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo assembly of the metallocofactor of C. ammoniagenes R2F is ... ribonucle- otide binding and radical transfer from the tyrosyl rad- ical (Y122) of the R2 subunit to the cysteine (C439) found in the active site of t...
Ngày tải lên : 15/02/2014, 01:20
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Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis Functional consequences for the enzyme ppt

Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis Functional consequences for the enzyme ppt

... different kinetic and thermodynamic properties of the tetrameric enzyme may result in fine-tuning of the properties of skeletal LDH-A, which is in line with previously observed kinetic and thermodynamic ... located in the sub- unit contact area of the tetramer, whereas the remaining two mismatches sur- round the contact area; this is expected to manifest in t...
Ngày tải lên : 19/02/2014, 02:20
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx

Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx

... short interfering RNA (siRNA) in human cells. Both HO-2 mRNA and protein are expressed in the eight human cancer cell lines examined, and HO-1 expression is detectable in five of the cell lines, including ... analyzed the expression profiles of HO-1 and HO-2 in eight human cell lines by northern and western blot analyses (Fig. 1A,B). Expression of H...
Ngày tải lên : 19/02/2014, 05:20
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Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

... [4–6]. In order to improve the biological production method, a detailed understanding of the biosynthesis of triterpene saponins is required, including the enzymes catalyzing the sequence of reactions ... reactions and the genes encoding these enzymes. The biosynthesis of triterpene saponins involves the initial cyclization of 2,3-oxidosqualene, a common pre- curso...
Ngày tải lên : 19/02/2014, 07:20
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Tài liệu Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix docx

Tài liệu Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix docx

... (HUC-F cells) and WI-38 cells. Expression of the human Mcm2 gene To understand the mechanism behind the enhanced expression of Mcm proteins in HeLa cells, the concentra- tions of Mcm2 mRNA in the ... Blue staining. Table 2. Quantitation and comparison of proteins in GM and WI-38 cells. From the data in Fig. 2 and others, the concentrations of Mcm...
Ngày tải lên : 20/02/2014, 23:20
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Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

... chain corresponding to the catalytic domain (C domain); and the B chain corresponding to the translocation domain (T domain) and the receptor-binding domain. The C and T domains remain covalently linked ... of the pro- tein in the N state and the evolution of the overall structure of the protein in the MG state. This degree of resolution is unpreceden...
Ngày tải lên : 16/02/2014, 09:20
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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

... mitochondria-targeting signal in the yeast system. Mitochondrial localization of intact CYP2E1 in yeast cells Because of the existing ambiguity in the literature on the nature and extent of CYP2E1 import into ... were initiated by the addition of 3 mm NADPH, and incubation was continued for 30 min at 37 °C in a shaking water bath. The reactions were terminated...
Ngày tải lên : 18/02/2014, 16:20
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