Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt
... ligand-binding potential of the active site, enabling
differentiation between the active site properties of
target enzymes to be made. This study probes the roles
of the active site histidines and lysine ... Wettstein-Knowles et al.
710 FEBS Journal 273 (2006) 695–710 ª 2006 The Authors Journal compilation ª 2006 FEBS
Fatty acid synthesis
Role of active sit...
... sphingenine-containing sphingolipids,
compared with 7% sphinganine-containing sphingoli-
pids. Thus, 93% of T. pseudonana LCBs contain one
or more double bonds.
Discussion
Using a combination of ... long and encodes a protein of
474 amino acids. Alignment with the corresponding
genomic sequence confirmed the presence of a 99 bp
intron in the TpdesO gene. The amino acid sequence...
... bead
characteristics, in which increases in molecular mass and
DA resulted in efficient loading and minimization of the
release of entrapped lipase [30]. The observed increase in
specific activity with increasing ... convoluted
spectral band around 3600–3000 cm
)1
in the spectra of both
chitosan and LMWC indicated the absence of free -OH
groups and the involvement of...
... glycocholic acid;
GCDA, glycochenodeoxycholic acid; I-BABP, human ileal bile acid- binding protein; iLBP, intracellular lipid-binding protein; L-BABP, chicken
liver bile acid- binding protein.
FEBS ... NMR-
based modeling and binding studies of a ternary com-
plex between chicken liver bile acid binding protein and
bile acids. Proteins 69, 177–191.
6 Pedo
`
M, D’Onofrio M,...
... December
2008)
doi:10.1111/j.1742-4658.2009.06866.x
l-Canavanine occurs as a toxic non -protein amino acid in more than 1500
leguminous plants. One mechanism of its toxicity is its incorporation into
proteins, replacing l-arginine and giving rise ... guanidinium g-N but also
comes into contact with the adenosine ring of Ade76.
There is a general consensus that tRNA binding is not
r...
... red; glycine and proline – black; valine,
leucine and isoleucine – grey; phenylalanine and tyrosine – blue; tryptophan – magenta; histidine – cyan; arginine and lysine – green; cysteine
– yellow.
M. ... features often omitted in analyses
of GH13 enzymes, but also clearly conserved in rBAT
and 4F2hc proteins, e.g. the b8-strand segment of TIM
barrel domain); and (c) prot...
... presence of kringle 5 in
the structure of the protease; miniplasmin (des-kringle 1-4 plasmin) is as
sensitive to fatty acids as plasmin, whereas the activity of microplasmin
(des-kringle 1-5 plasmin) ... Amidolytic activity of des-kringle plasmin derivatives in the presence of fatty acids. The activity of 20 nM miniplasmin (A) and micro-
plasmin (B) on 120 l
M Spectrozym...
... (cleavage site and affinity
site) and six calcium-binding sites, three of which are
high-affinity binding sites responsible for enzyme sta-
bility and three are low-affinity sites regulating the cat-
alytic ... presence of Ca
2+
increased the thermal stability of PhyH and PhyH-DII.
After incubation at 60 °C for 30 min, both enzymes
retained > 70% of their initial activity...
... full-length
v-KIND; DKIND1, deletion of KIND1;
DKIND2, deletion of KIND2; DKIND1 + 2,
deletion of both KIND1 and KIND2; DRasN,
deletion of RasN; DGEF, deletion of
RasGEF; KIND1, KIND1 domain; KIND2,
KIND2 ... resides
within the 32 residues (amino acids 456–487) of the
v-KIND KIND2 domain.
To examine whether the MAP2 binding core site of
v-KIND binds to intact MAP2 protein,...
... lectin profiling (Fig. 3C). In this
system, 100 ng or less of each candidate is immunopre-
cipitated from serum using an antibody against the
core protein moiety of the candidate glycoprotein (Step
1 ... of
this database, we have included a full list of N-glycopro-
teins from C. elegans and a partial list from mouse liver
containing the protein (gene) ID, protein name, glycos...