Tài liệu Báo cáo khoa học: Structural and functional specificities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx
... between the C tails of
b and c and the ubiquitin ligase Nedd4. The WW domains
of Nedd4 bind to the proline-rich PY motifs of b and
cENaC, leading to channel ubiquitination, internalization
and degradation ... donor (Fig. 8A), the inhibitory effects of heparin
and DPG (Fig. 8B), the fact that purified CK2 can
phosphorylate bS631 and cT599 (Fig. 7A), the presenc...
... pyruvate, and meth-
ylamine are bound to the enzyme in this order, and
N-methyl-l-alanine and then NADP
+
are released from
the enzyme. Therefore, the kinetic mechanism of the
enzyme is the same ... amino-acid sequences of the gene
showed high identities (97.9% and 99.1%, respectively)
with those of the PP3591 gene of P. putida KT2440.
Purification and subunit s...
... hypoxanthine and guanine is only 0.26% and
0.09%, respectively, of the activity of HPRT, the role
of the PRTFDC1 in purine metabolism remains
unclear and it is uncertain whether it has the capacity
to ... II. The hood domain is mainly built
up by residues from the C-terminus and consists of a
two-stranded anti-parallel b-sheet composed of b2 and
b9 and an a-h...
... distribu-
tion of 100 l
M proinsulin C-peptide in the
presence of divalent Ca
2+
and Mg
2+
ions
under native conditions (B), and of 100 l
M
C-peptide in the presence of insulin (C) and
of 100 l
M ... SDS to structurally character-
ize the oligomerization process of C-peptide and ana-
lyzed the formation of oligomers and their secondary
structure by complementar...
... hinge domains
The composition of the two hinges that connect the
FMN subdomain in NOS enzymes (H1 and H2 in
Fig. 5) defines the allowable movements of the FMN
subdomain and thus controls the FMN–NOSoxy ... further test the validity, kinetics and thermodynam-
ics of the through-heme pathway in NOS enzymes.
Conclusions
Although the NOS flavoprotein domain has fundame...
... Klinman and cowork-
ers to study the viscosity dependence of the rate of
hydride transfer in GO [70,71]. These authors studied
various glycoforms of the enzyme (varying in the
extent of glycosylation) ... Comparison of
rates and kinetic isotope effects using PEG-modified
variants and glycoforms of glucose oxidase: the
relationship of modification of the protein...
... orientation
of the N5 of the flavin, the hydride to be transferred,
and C4 of the nicotinamide. In FNR, displacement
of the C-terminal Tyr appears to be required for the
interaction to occur [117,118]. The ... because the semiquinone H-bond with the CO
of Asn is weaker than the bond formed with the sma-
ller Gly, and because of the presence in the oxid...
... N10 and E97 of the second
subunit. The backbone NH group of R98 forms a
hydrogen bond with the backbone CO of F102. Fur-
thermore, the orientation of the side chain of the two
residues brings the ... nature. The growing body
of sequence and structural data on these well-studied
enzymes affords an opportunity to evaluate the conse-
quences of mutations. In...
... indicate the starting and
ending residues of the secondary structural elements. (C) The
domain-swapped dimer of St SurE. The A and B subunits are
shown in green and blue, respectively. The darker and ... as in archaic and thermophilic homo-
logues. The St SurE monomer consists of 13 b-strands,
six a-helices and three 3
10
-helices. The core of the pro-
te...
... to the side chain of Asp70, and one
from the backbone N of Gly63, and the phosphate
group forming hydrogen bonds from O1 to Arg55,
from O2 to the backbone N and the side chain of
Thr138, and ... to the
three lysines (Fig. 3E). There is no water molecule fix-
ing the three lysines from the B, C and F subunits,
and there is no direct interaction between subunits...