... time-dependent enhance-
ment of the 57-kDa band with a concomitant diminu-
tion of the 38-kDa and 19-kDa bands, as analyzed by
SDS ⁄ PAGE (Fig. 3A). The 38-kDa and 19-kDa bands
did not disappear completely ... isomorphous with those
of the uninhibited wild-type enzyme and of the E78H
mutant, and the structures are very similar (r.m.s.d.
0.264 and 0.312 A
˚
for 350 Ca p...
... Protein–ligand and water-mediated protein–ligand hydrogen bond interactions (broken lines) that stablize the pantoate molecules in
site I and site II of nPS. Oxygen atoms of pantoate and water ... changes from ) 75.20° to )171.65° and )89.25°
in the A-chain and B-chain of nPS, and in the case of
His37, the v
1
torsion angle changes from 63.34° to
)67.80° and )52.11°...
... 177 and is involved in stabilizing strands b8 and b9.
Ca4 is the weak binding site in PRK, which is formed by
the side chain of Asp260 and the carbonyl oxygen of
Thr16, and stabilizes the N- and ... VPRK and SPRK have an
insert of three residues. Ca3 is the so-called strong bind-
ing site found in PRK and formed by the side chain of
Asp200 and the carbonyl oxygen...
... fatty
acids affected the activity of miniplasmin in the same
manner as that of plasmin; apparent activation was
seen with stearate and inhibition was seen with oleate
and arachidonate (Fig. 8A). ... lLof1nM plasmin solution containing
oleic acid, at concentrations (in l
M) indicated by the numbers, and the absorbance at 405 nm was measured. The mean and standard deviation
(d...
... supported by grants from the Spanish
Ministry of Education and Science (BIO2007-67885
and BIO2010-17039) and the Research Teams Consoli-
dation Programme of the UCM, Research Group
920267 and the Iberoamerican ... Inhibition of PLA2 activity by MA. MA and 3,4-dichloroiso-
coumarin (DIC) were tested at 100 and 400 l
M. The compounds
were incubated with the enzyme control...
... accompanied by a suppression in energy
expenditure. These data suggest that CPT1c can
integrate carbohydrate and lipid nutrient sensing in the
brain and is an example of an enzyme that can sense
and ... Consistent with this is the close
correlation with the hypothalamic levels of orexigenic
and anorexigenic neuropeptide expression during fast-
ing and refeeding. A detail...
... assembly and export of N-oxide
reductases. Biochem Soc Trans 33, 124–126.
29 Zhang B, Zhang Y, Wang Z & Zheng Y (2000) The
role of Mg
2+
cofactor in the guanine nucleotide
exchange and GTP ... co-
expression of active torD restores the Tat proofreading
of this enzyme and so rescues hydrogenase-2 activity
in the mutant strain. RJ607 was transformed with a
pUNIPROM vecto...
... normal
and cancerous cells [9], and ubiquitously distributed in
the nucleolus, nucleus and cytoplasm of the cell, as
well as at the cell surface [10]. Nucleolin ⁄ C23 regulates
many aspects of DNA and ... cells transfected with the control oligo-
mers and much lower at 13 and 44% in cells transfect-
ed with the nucleolin ⁄ C23 antisense oligomer
(Fig. 6C). Second, the act...
... unlabeled wild-type oligonucleotides (lanes 3 and 8) and mutated oligonucleotides (lanes 4 and 9) were
used. Lanes 1 and 5 show the labeled oligonucleotides incubated without protein.
Sox9 regulates ... expression of AP-2e
in hypertrophic chondrocytes is regulated, the sequence
of the AP-2e promoter was analyzed, and binding sites
for the transcription factors AP-2a and Sox9 w...
... loop) in the active site region and the S1
pocket, whereas the intramolecular connections, for
instance between the TF-binding region and the active
site and between the TF-binding region and the ... activity and inhibitor
susceptibility, native and active site- inhibited G372A-FVIIa bound sTF with
the same affinity as the corresponding forms of FVIIa, and burial...