Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx

Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx

Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx

... time-dependent enhance- ment of the 57-kDa band with a concomitant diminu- tion of the 38-kDa and 19-kDa bands, as analyzed by SDS ⁄ PAGE (Fig. 3A). The 38-kDa and 19-kDa bands did not disappear completely ... isomorphous with those of the uninhibited wild-type enzyme and of the E78H mutant, and the structures are very similar (r.m.s.d. 0.264 and 0.312 A ˚ for 350 Ca p...
Ngày tải lên : 19/02/2014, 07:20
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Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

... Protein–ligand and water-mediated protein–ligand hydrogen bond interactions (broken lines) that stablize the pantoate molecules in site I and site II of nPS. Oxygen atoms of pantoate and water ... changes from ) 75.20° to )171.65° and )89.25° in the A-chain and B-chain of nPS, and in the case of His37, the v 1 torsion angle changes from 63.34° to )67.80° and )52.11°...
Ngày tải lên : 16/02/2014, 09:20
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Tài liệu Báo cáo khoa học: ˚ The 1.8 A crystal structure of a proteinase K-like enzyme from a psychrotroph Serratia species docx

Tài liệu Báo cáo khoa học: ˚ The 1.8 A crystal structure of a proteinase K-like enzyme from a psychrotroph Serratia species docx

... 177 and is involved in stabilizing strands b8 and b9. Ca4 is the weak binding site in PRK, which is formed by the side chain of Asp260 and the carbonyl oxygen of Thr16, and stabilizes the N- and ... VPRK and SPRK have an insert of three residues. Ca3 is the so-called strong bind- ing site found in PRK and formed by the side chain of Asp200 and the carbonyl oxygen...
Ngày tải lên : 19/02/2014, 07:20
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Tài liệu Báo cáo khoa học: Suppressed catalytic efficiency of plasmin in the presence of long-chain fatty acids Identification of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt

Tài liệu Báo cáo khoa học: Suppressed catalytic efficiency of plasmin in the presence of long-chain fatty acids Identification of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt

... fatty acids affected the activity of miniplasmin in the same manner as that of plasmin; apparent activation was seen with stearate and inhibition was seen with oleate and arachidonate (Fig. 8A). ... lLof1nM plasmin solution containing oleic acid, at concentrations (in l M) indicated by the numbers, and the absorbance at 405 nm was measured. The mean and standard deviation (d...
Ngày tải lên : 18/02/2014, 17:20
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Tài liệu Báo cáo khoa học: Multi-targeted activity of maslinic acid as an antimalarial natural compound pdf

Tài liệu Báo cáo khoa học: Multi-targeted activity of maslinic acid as an antimalarial natural compound pdf

... supported by grants from the Spanish Ministry of Education and Science (BIO2007-67885 and BIO2010-17039) and the Research Teams Consoli- dation Programme of the UCM, Research Group 920267 and the Iberoamerican ... Inhibition of PLA2 activity by MA. MA and 3,4-dichloroiso- coumarin (DIC) were tested at 100 and 400 l M. The compounds were incubated with the enzyme control...
Ngày tải lên : 14/02/2014, 14:20
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Tài liệu Báo cáo khoa học: Hypothalamic malonyl-CoA and CPT1c in the treatment of obesity pptx

Tài liệu Báo cáo khoa học: Hypothalamic malonyl-CoA and CPT1c in the treatment of obesity pptx

... accompanied by a suppression in energy expenditure. These data suggest that CPT1c can integrate carbohydrate and lipid nutrient sensing in the brain and is an example of an enzyme that can sense and ... Consistent with this is the close correlation with the hypothalamic levels of orexigenic and anorexigenic neuropeptide expression during fast- ing and refeeding. A detail...
Ngày tải lên : 14/02/2014, 22:20
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Tài liệu Báo cáo khoa học: Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping ppt

Tài liệu Báo cáo khoa học: Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping ppt

... assembly and export of N-oxide reductases. Biochem Soc Trans 33, 124–126. 29 Zhang B, Zhang Y, Wang Z & Zheng Y (2000) The role of Mg 2+ cofactor in the guanine nucleotide exchange and GTP ... co- expression of active torD restores the Tat proofreading of this enzyme and so rescues hydrogenase-2 activity in the mutant strain. RJ607 was transformed with a pUNIPROM vecto...
Ngày tải lên : 16/02/2014, 09:20
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Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx

Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx

... normal and cancerous cells [9], and ubiquitously distributed in the nucleolus, nucleus and cytoplasm of the cell, as well as at the cell surface [10]. Nucleolin ⁄ C23 regulates many aspects of DNA and ... cells transfected with the control oligo- mers and much lower at 13 and 44% in cells transfect- ed with the nucleolin ⁄ C23 antisense oligomer (Fig. 6C). Second, the act...
Ngày tải lên : 16/02/2014, 09:20
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Tài liệu Báo cáo khoa học: The cartilage-specific transcription factor Sox9 regulates AP-2e expression in chondrocytes pptx

Tài liệu Báo cáo khoa học: The cartilage-specific transcription factor Sox9 regulates AP-2e expression in chondrocytes pptx

... unlabeled wild-type oligonucleotides (lanes 3 and 8) and mutated oligonucleotides (lanes 4 and 9) were used. Lanes 1 and 5 show the labeled oligonucleotides incubated without protein. Sox9 regulates ... expression of AP-2e in hypertrophic chondrocytes is regulated, the sequence of the AP-2e promoter was analyzed, and binding sites for the transcription factors AP-2a and Sox9 w...
Ngày tải lên : 18/02/2014, 08:20
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Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf

Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf

... loop) in the active site region and the S1 pocket, whereas the intramolecular connections, for instance between the TF-binding region and the active site and between the TF-binding region and the ... activity and inhibitor susceptibility, native and active site- inhibited G372A-FVIIa bound sTF with the same affinity as the corresponding forms of FVIIa, and burial...
Ngày tải lên : 18/02/2014, 08:20
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