... 2006 FEBS
Biochemical characterization of human
3-methylglutaconyl-CoA hydratase and its role in leucine
metabolism
Matthias Mack
1
, Ute Schniegler-Mattox
2
, Verena Peters
3
, Georg F. Hoffmann
3
, ... 3-MG-CoA
hydratase reaction of leucine catabolism at the protein
and DNA levels and developed a novel assay for
enzyme analysis in a diagnostic setting. Th...
... under aerobic and anaerobic condi-
tions, in the range of 0.6 lmolÆmg
)1
Æmin
)1
. No evidence was
obtained for redox cycling of FMN. The amino acid
residues involved in the FMN binding site are ... M15-pQEidi2.
Preparation of the recombinant Idi-2 protein
The recombinant E. coli strain M15-pQEidi2 was grown in
Luria-Bertani broth containing ampicillin (180 mgÆL
)1
)and
kanamy...
... characterization of
HpSDH demonstrates its activity with k
cat
of 7.7 s
)1
and K
m
of 0.148 mm
toward shikimate, k
cat
of 7.1 s
)1
and K
m
of 0.182 mm toward NADP, k
cat
of 5.2 s
)1
and K
m
of ... com-
prising two domains that are responsible for binding
substrate and NADP cofactor. The detailed structural
information might expedite the discovery of novel
SDH inhib...
... proteins are a large family of RNA-binding
proteins found in all eukaryotes. These proteins reduce
the expression of mRNA targets by binding in 3¢ UTR
regulatory elements, thus controlling translation ... cis-acting elements usually
located in the 3¢ UTR. In recent years, and as a result
of great efforts aiming to understand the mechanism
of RNA control in animals, the fun...
... ligand binding, and the role of
storage proteins, the cupins constitute one of the most
diverse superfamilies of proteins known. They have
been described from all three domains of life [1,2], and
usually ... Straganz and Bernd Nidetzky
Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Austria
Introduction
In terms of their physi...
... earlier, a value of 2.8 · 10
7
Æm
)1
Æmin
)1
was
obtained for the second order rate constant k
2
⁄ K
M
.
Using this value of k
2
⁄ K
M
and the values of k
2
and
k
)1
(4.1Æmin
)1
and 0.9Æmin
)1
respectively), ... 3¢-terminal guanosine of the
ribozyme as the first-step nucleophile, whereas self-splicing utilizes an exog-
enous guanosine. To further aid in our understanding of r...
... band
appears at around 660–675 nm; this increases in inten-
sity with time, indicating the formation of biliverdin.
In this case, neither bands of the oxy form (at 541 and
578 nm) nor bands of ... con-
verts combined heme into biliverdin IX
a
, retaining
a-regiospecificity, and releasing CO and free iron, in
the presence of oxygen and NADPH ⁄ FNR ⁄ Fd, with-
out requiring...
... mechanism of ligand–receptor interaction has been
proposed for all chemokines. Binding involves interac-
tions between the chemokine ligand N-loop and the
receptor N-domain, and ligand N-terminal and ... major role in
determining binding affinity and not in ligand selectiv-
ity [22–24].
Design and characterization of IL-8(1–66)
monomer
Previous studies using a ‘trapped’ m...
... consisting of four copies of four
subunits, a, b, c and d. The a and b subunits are regu-
latory and are the sites of phosphorylation and meta-
bolite binding and are also regulated by the binding
of ... extension to the protein kinase folds back
on the kinase domain and interferes with the substrate
binding sites. In the case of the titin and twitchin
kinases, the a...
... Dashed
lines indicate van der Waals contacts with interatomic distances in A
˚
. Dotted lines indicate C-HÆÆÆO interactions. Black lines indicate interac-
tions in SQV complexes. Red lines indicate interactions ... different inhibitors will give insight into
the mechanisms of resistance and help in the design of
new inhibitors.
Results
APV inhibition of PR and mutants
The kine...