Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
... utilization of two
transcription-starting exons and the involvement of Sp1
in its transcriptional regulation
Yoshihiko Kominato
1
, Misuzu Ueki
2
, Reiko Iida
3
, Yasuyuki Kawai
4
, Tamiko Nakajima
1
, ... role in promoter activity in the 5¢-upstream
region of DNASE1 exon 1a.
Results
Mapping of transcription initiation sites in the
human DNASE1 gen...
... phosphatidylcholine synthesis is reduced or
in the presence of phosphatidylcholine degradation
[18,25]. In particular, inhibition of the final step of
phosphatidylcholine synthesis via the Kennedy ... Variations in intensity of the examined signals are not
linear with time and ⁄ or cell proliferation [13]. It is therefore
necessary to examine the signal intensity for...
... classification, based only on
similarities in amino acid sequences, groups together
chitinases and proteins devoid of catalytic activity due
to the substitution of a critical amino acid in the cata-
lytic ... transcripts during
development and in adult tissues
To gain insights into possible physiological functions
of Cg-Clp2, determination of its tissue distribution an...
... C-terminus of isoform 1 of the PM H
+
-ATPase
of A. thaliana (AHA1) interacts with the first 88 amino
acids of PPI1 [22], indicating that the PM H
+
-ATPase
binding site of PPI1 is localized therein. ... therein. Indeed, fusion
proteins containing the first 88 amino acids of PPI1,
linked either to a His-tag (His
6
PPI1
88
) or to GST,
interact with A. thaliana H
+
-ATPase i...
... structure is located
in the C-terminal part of the enzyme.
The subtilisin-like peptidases are dependent on cal-
cium to maintain their stability, and PRK contains
two calcium binding sites, one ... preregion is indicated in red, the
pro-region in black, catalytic domain in blue
and the C-terminal domains in violet. The
assumed start of the second C-terminal
d...
... Characterization of the products of the genes
SNO1
and
SNZ1
involved
in pyridoxine synthesis in
Saccharomyces cerevisiae
Yi-Xin Dong, Shinji Sueda, Jun-Ichi Nikawa and Hiroki Kondo
Department ... a
glutaminase to supply ammonia for the ring nitrogen of
pyridoxine in yeast. Based on these and other lines of
evidence, a putative synthetic pathway to pyridoxine is
pre...
... is
presented in Fig. 1. In order to gain further insights
into the modes of regulation of TPb, the aim of the
current study was to map the minimal transcriptional
unit and to identify the key regulatory ... receptors in humans is indeed
unclear, there is mounting evidence that they undergo
differential signaling and regulation, strengthening the
viewpoint that...
... detail in both the experimental procedures
(K
i
of cyanidin, bilirubin, biliverdin, section B) and in Table 2 (K
d
of the complexes of bilitranslocase with bilirubin, nicotinic acid and cyanidin
3-glucoside, ... to track the progress of the antibody-induced
inhibition. Figure 5A shows that increasing bilirubin
concentrations more and more retarded the progress o...
... substrate,
thereby facilitating binding to the substrate-binding
cleft. It is obvious that elucidation of the 3D structure of
PelB would give more insight into structural organiza-
tion of the binding ... characteristics
PelB was examined by incubation with polygalacturonic
acid (PGA) following standard assay conditions. The
experiments showed an increase in the amount of...
... effective inhibition of
binding of ICAM-4 positive red cells by anti-ICAM-4
antibodies, indicate a major role for ICAM-4 in binding of
red cells to the I domains. The efficient inhibition of the
Fig. ... contain binding sites
for ICAM-1 and ICAM-2. Further proof for the interaction
of ICAM-4 with these I domains was obtained by compar-
ing the ability of recombina...