Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis Functional consequences for the enzyme ppt
... 2007 The Authors Journal compilation ª 2007 FEBS 1513
Differential expression of duplicated LDH-A genes during
temperature acclimation of weatherfish Misgurnus fossilis
Functional consequences for ... white skeletal muscle of weatherfish, Misgurnus fossilis.
Two isoforms of LDH-A mRNA were isolated and characterized: a short iso-
form (mRNA
a
ldhÀa
¼...
... subunits for catalysis, as expected.
The tyrosyl radical of the R2F subunit was also
monitored during the course of the enzyme assay.
Curiously, under conditions where the product was
formed, the ... species. Here,
the source of the native R2F-protein of the C. ammonia-
genes ribonucleotide reductase were transformants
from the reintroduction of the nrd...
... the possibility existed for dis-
tinct functions for these two isoforms. In the present
study, we have compared further the Mxi1-SRa and
Mxi1-SRb isoforms at the levels of Myc antagonism in
the ... assay. Expression constructs were
generated encoding Myc-tagged versions of these two
isoforms, as well as of the Mxi1-WR isoform that lacks
the SID, and shown to give ris...
... mark the
boundary of the predicted substrate-binding pocket,
reveals that some of them are conserved in the inac-
tive isoform (i.e. Trp191 and Phe294). Even though
the precise role of these ... a metal-ion cofactor and is able to
take up the metal from the expressing host during
protein synthesis [21,33], we investigated whether the
production of the human recombina...
... with rabbit anti-mouse Igs for 30 min, followed by a
further 30 min with the APAAP conjugate. These steps were
repeated for 10 min each to increase the intensity of the sig-
nal. After three additional ... loading of HepG2 cells resulted in increased expression of
Nor3.2-reactive CD1d molecules at the plasma membrane. Expression of
classical MHC class I and II molecule...
... mediates activation
of Ras subsequent to the stimulation of G
i
- and G
q
-
coupled receptors [8,9].
The main mechanism for the activation of Ras-
GRF1 is the binding of Ca
2+
⁄ CaM to the N-terminal
IQ ... [17,19]. The smaller iso-
forms correspond to N-terminal deletions of the full-
length 140 kDa protein. The physiological role of the
guanine nucleotide exc...
... several other transformed cell lines, although the extent of
the enhancement varied among the cells. The enhanced level
of Mcm expression in these transformed cell lines would
contribute to the growth ... expressed in the larger nuclei of cancer cells
compared with those in basal cells of the normal squamous
cell epithelial layer (Fig. 7). The ubiquitous nature of...
... up-regulation of
survivin expression [52]. The levels of cIAP-2 are
down-regulated by gefitinib or erlotinib in intestinal
epithelial cells [53]. Furthermore, the expression of
cIAP-1 and of X-linked ... family genes and the phos-
phorylation state of their proteins, thereby changing
the amount and localization of Bcl-2 family members.
However, each type of cancer h...
... assembly of for-
oxymithine by a synthetase lacking all E-domains. The
lack of sequence information for the S. nitrosporeus
genome impeded the identification of a biosynthetic
machinery governing foroxymithine ... to the identification of the second siderophore
(data not shown). We therefore assume that either
the extraction conditions were inadequate for the iso-
la...
... decreasing
the activity of caspase-3 in the three cell lines.
Effect of HSS knockdown on CCCP-induced
apoptosis
To further elucidate the functional role of HSS in
CCCP-induced apoptosis, the expression ... are the energy producers of the cell, and
are essential for the maintenance of cell life. However,
in the last 10 years, it has also become apparent that
mit...