... ascomycete fungal laccase from
Thielavia arenaria – common structural features of
asco-laccases
Juha P. Kallio
1
, Chiara Gasparetti
2
, Martina Andberg
2
, Harry Boer
2
, Anu Koivula
2
, Kristiina Kruus
2
,
Juha ... weak homodimer, as observed for MaL, that may determine
the properties of these asco-laccases at high protein concentrations.
Database
Structural data are available in the...
... by the importin -a
nuclear import pathway, provided that CLIC4 can undergo a conforma-
tional rearrangement that exposes the NLS in an extended conformation.
Database
Structural data are available ... Koochek A, Ryscavage A,
Bhat K, Tanaka T, Oshima A, Fitzgerald P & Yuspa
SH (2007) Reciprocal modifications of CLIC4 in tumor
epithelium and stroma mark malignant progression of...
... bound to the metal, in the company of
a water oxygen, from the apical positions. The manga-
nese was only 0.06 A
˚
out of the equatorial plane
(Table 3). The angles around the metal cofactor sug-
gested ... hyper-thermophilic crenarchaeon, Aeropyrum
pernix K1. DNA Res 6, 83–101.
11 Nakamura T, Yamamoto T, Inoue T, Matsumura H,
Kobayashi A, Hagihara Y, Uegaki K, Ataka M,...
... phytate with pH optima in the
acidic range. They consist of two domains, a large a ⁄ b
domain and a small a domain with the catalytic site at
the interface of the two domains [4,5]. HAPs can initi-
ate ... substrate-free
AppA the C
a
atoms are 2.41 A
˚
apart, whereas for the
substrate-free PhyK and the substrate-loaded AppA
the averaged distance is only 1...
... FEBS
Crystal structure of the catalytic domain of DESC1, a new
member of the type II transmembrane serine proteinase
family
Otto J. P. Kyrieleis
1,
*, Robert Huber
1,2
, Edgar Ong
3
, Ryan Oehler
3
, ... on surface analysis, we propose a rigid
domain association for the N-terminal SEA domain with the back site of
the proteinase domain.
A...
... Ivanova N, Sorokin A, Anderson I, Galleron N,
Candelon B, Kapatral V, Bhattacharyya A, Reznik G,
Mikhailova N, Lapidus A et al. (2003) Genome
sequence of Bacillus cereus and comparative analysis
with ... 276–280.
7 Handa N, Terada T, Kamewari Y, Hamana H, Tame
JRH, Park S-Y, Kinoshita K, Ota M, Nakamura H,
Kuramitsu S et al. (2003) Crystal structure of the con-
served protein TT...
... it is part of a molecular strategy of cold
adaptation. Because of the ordering of water structure
at low temperature (i.e. below approximately the tem-
perature of maximum stability) the entropic ... replacement
using a homology model based on the known structure
of proteinase K (PDB accession number, 1IC6) as a
search model. The crystallized 30 kDa catalyti...
... oligonucleotides:
a sense primer 5¢-CAACAAATTTG
CATATGACTATT
AAAG-3¢ containing an NdeI site (underlined) next to the
start codon of the T. brucei gGAPDH gene; an anti-
sense primer 5¢-CAGCCAAGCG
CCTAGGGAGCGAGA
AC-3¢, ... Mikaelian & Sergeant [33] and
using the Vent DNA polymerase. The T. brucei gGAPDH
Thr196 ACA codon was changed into the Ala codon GCA,
and the Thr225 cod...
... indole-3-acetic acid (IAA), which is synthe-
sized by plants [1,2] and plant-associated bacteria [3,4].
Several pathways for the synthesis of IAA in these
organisms have been described, and most of them ... protein atoms. The final R-values
and other refinement statistics are given in Table 1. The
X-ray data and the atomic coordinates have been deposited
at the Protein Data Bank,...
... should favor a
salt bridge interaction that will decrease the pK
a
of the
aspartic acid and increase the pK
a
of the histidine side
chain groups. Finally, E66 and R95 are in their canon-
ical positions ... in
HtA replaces a tyrosine of a- sarcin, and the aromatic
ring of F126, close to the catalytic H113, replaces a
leucine side chain in a- sarcin in a simi...