Tài liệu Báo cáo khoa học: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr docx
... with a construct spanning 4.9 kb upstream
of the transcriptional start site strongly indicate differ-
ences in transcriptional regulation. The strong synergy
generated by the combination of FGF-2 ... For other controls, pri-
mary or secondary antibodies were omitted.
Acknowledgements
The authors are obliged to Professor Rolf Howlett for
the preparation and donation of wound...
... compute the frequency of all the
candidates identified in all snippets, and output the
one with the highest frequency.
4 Experiments and Evaluation
We extracted the Wikipedia titles of English and ...
translations in another language. By retrieving and
identifying such translation counterparts on the
Web, we can cope with the OOV problem.
Consider the tec...
... Dicer protein [35]. As expected, the band was
shifted in the presence of the Dicer antibody but not
the other antibodies.
We also wished to confirm the location of any
TRBP-containing bands, if these ... the impact of silencing
TRBP and Dicer on the function of the fully paired
and mismatched sequences, we wished to examine
Fig. 1. Effect of guide strand 5¢...
... 3783–3792 ª 2008 The Authors Journal compilation ª 2008 FEBS
Effect of ionic strength and oxidation on the P-loop
conformation of the protein tyrosine phosphatase-like
phytase, PhyAsr
Robert J. ... whether the move-
ment of the P-loop in PhyAsr is due to oxidation of
the catalytic cysteine, we oxidized crystals of PhyAsr
Table 1. Effect...
... expression, acetylation of H3 and H4
histones, and the remaining DHSs in the deleted cells
were examined to determine the roles of these DHSs
on the transcription of Ig-b gene and on the mainten-
ance ... of the South-
ern hybridization. Position of the probes, the size and position of restriction fragments hybridizable with the probe, and exon ⁄ in...
... pore [10]. Results about the effect of the
luminal (trans) calcium on the activity of the RyR1 are
controversial depending on experimental conditions.
Inhibition of the channel was described ... Gd
3+
and a Hill coefficient
of 4.7 ± 0.8.
We have also tested the effect of Gd
3+
on the lumi-
nal side of the reconstituted channel. To decrease the
possibil...
... under low ionic strength conditions [8,44].
However, this effect is not seen in the kinetics of the
MA/CA hydrolysis. In the high ionic strength of the
assays the dimerization is strengthened as ... gene. Many of these mutations are located in the
substrate binding site of the PR, and these mutations
have considerable impact on PR activity and specificity....
... 97.4
3228.9
837.7
estimated. In the second approach, on the other
hand, as described in the previous section for the
case of acquisition from (pseudo-) parallel cor-
pora, the candidates of bilingual term ... measured, and their curves against the changes
of
Ld
are shown in Figure 2. Let
DP„f
denote
the set of reference article pairs within the range
of dates...
... neuron degeneration
In 2001, Oosthuyse et al. [18] reported that deletion of
the hypoxia-response element of the VEGF-A gene
promoter and a reduction in VEGF-A expression
cause the degeneration of ... tissue
fluid and edema in the brain may be dangerous
because the volume of the brain tissue is tightly limited
by the cranial bone. This limitation is unique among
tissu...
... polarization is further evidence that the functions
of Vrp1p in endocytosis, cytokinesis, and growth at
elevated temperature, on the one hand, and in cortical
actin-patch polarization, on the other hand, ... level of the untagged pro-
teins, but we expect that the relative expression level of
the tagged proteins is indicative of that of the equival-
ent untagge...