Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf
... Journal compilation ª 2008 FEBS 2631
Kinetic analysis of effector modulation of
butyrylcholinesterase-catalysed hydrolysis of acetanilides
and homologous esters
Patrick Masson
1
, Marie-The
´
re
`
se ... carboxyles-
terases and albumin, was controlled by nondenaturing
PAGE and activity staining [26,68].
Kinetics of substrate hydrolysis in the absence
of lig...
... presence (s) and absence (d )of4 l
M ChlH.
K
app
MgD
¼ 17.3 ± 3.3 lM in the presence of ChlH. K
app
MgD
¼
24.3 ± 7.5 l
M in the absence of ChlH.
Fig. 3. Quenched-flow ⁄ HPLC analysis of product and intermediate
evolution. ... third subunit, analysis of
the interaction of the three subunits with the yeast two-
hybrid system, and reconstitution of the enzyme activity
by co-...
... Quantitative analysis of the experimental O–J–I–P
chlorophyll fluorescence induction kinetics
Apparent activation energy and origin of each kinetic step
Steve Boisvert, David Joly and Robert Carpentier
Groupe ... thylakoid
membranes. Such analysis is needed for a a more thor-
ough use of FI in the study of PSII electron transport
and to obtain a more complete analysis of...
... Centre of Neuroscience, University of Insubria, Busto Arsizio, Italy
2 Department of Biotechnology, University of Verona, Italy
3 Department of Clinical and Biological Sciences, University of Insubria, ... b-gal and
a-syn cells treated or not with dopamine, as described
above. Statistical analysis, by two-way analysis of
β
β
-Actin
α
-Synuclein
Fig. 1. Relative expression...
... constant of
the 5¢ exon mimic, h is the fraction of 5¢ exon mimic bound
to the ribozyme, and [ribozyme]
u
is the concentration of
unbound ribozyme in the reaction.
Measurement of rate constant of
substrate-cleavage ... Tinoco I Jr (1982) Comparison of the
kinetics of ribooligonucleotide, deoxyribooligonucleo-
tide, and hybrid oligonucleotide double-strand forma-
tion by temp...
... Comparison of the hydrolysis reaction as a function of denaturing
agent and pH. (A), (B) and (C): (d), (s )and( .) correspond to
guanidinium chloride concentrations of 0 , 2 and 3
M
, respectively.
Hydrolysis ... 2.5, 8.0 and 11.5, and aliquots of each pH value were
incubated at 37 °C, 50 °Cand70°C, respectively. From
these, aliquots of 2 lL were taken at 0, 1, 2, 4, 6...
... absorption bands.
Fig. 2. (A) Absorption s pectra of CuTCPP in the absence and presence
of different concentrations of MCL and (B) difference absorption spectra
obtained by subtracting the spectrum of CuTCPP ... the presence of d ifferent c oncentrations of MCL. Tempera-
ture ¼ 20 °C.
Fig. 3. (A) Absorption spectra of CuTMPyP in the absence and pres-
ence of different concen...
... ÔStains-
AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4.
As the full-length Dp427 isoform of dystrophin does not
enter the second dimension of conventional 2D gels, the
expression level of ... viscosity of t he extract as a result of D NA, 2 lLof
DNase I (200 units) was added per 100 lL of buffer [30].
Following filtration through two layers of miracloth a n...
... DEC1 with BMAL1 and the bin ding to the E-box.
Substitution of Arg65 for Ala abolished the interaction of
DEC1 with BMAL1 and the binding of DEC1 to the E-box.
However, substitution of His57 for ... binding buffer and the
bound proteins were analyzed by SDS/PAGE and a uto-
radiography.
Results
Deletion analysis of DEC1 on its suppressive activity
in the presence of CLOCK...
... proportional increase in formate and
acetate production and a decrease in lactate produc-
Fig. 2. Modulation of PFK activity and the effects on growth and fluxes. (A) Library of strains with modulated ... lactis
consists of the three genes pfk, pyk and
ldh, coding for phosphofructokinase (PFK),
pyruvate kinase (PK) and lactate dehydro-
genase (LDH), respectively.
B. Koebman...