... used to establish that KCNE4
directly associates with KCNQ1, and can co-associate together with
KCNE1 in the same KCNQ1 complex to form a ‘triple subunit’ complex
(KCNE1–KCNQ1 KCNE4) . We also used ... interaction of
KCNE4 with KCNQ1. This hypothesis was tested by
examining whether KCNE4 forms protein complexes
with KCNQ1. KCNE4 and KCNQ1 were transiently
co-expresse...
... to the type I and type II receptors on the cell
surface. These receptors are serine ⁄ threonine kinase
receptors with a single transmembrane domain. Bind-
ing of the ligand to the receptor complex ... complex induces
the type II receptor to phosphorylate the type I
receptor, which then leads to activation of the type I
receptor. The signal is passed on to the substrate...
... of the long desk is a chair. Another chair
is to the left of the long desk. The chair in front
of the desk is near the short desk."
OTHER APPROACHES AND
CLOSING REMARKS
Nearly all the ... Intuitively, the closer the "stool" is
to the region of space defined by the projection of
the desk's dimensions into this space, the more the
spatial a...
... help the users to discern essential
information with unparalleled clarity.
The “Technologies behind the system” chapter
describes the implementation and the integration
approach of the core ... managing dynamic content-
driven websites with a linguistic
platform. The platform enriches the
content of these websites with revealing
details and reduces the manual wor...
... thetase to esterify the 13cROL generated in the reac-
tion. Therefore, 13cROL is accumulated in the absence
of LRAT in the reaction (Fig. S3B).
Under the in vitro assay conditions of the ... washes with NaCl ⁄ Tris with Tween 20,
the membrane was then incubated for 1.5 h with a
1 : 25 000 dilution of anti-mouse IgG conjugated with Dy-
Light 549 and anti-rabbit IgG conjug...
... mitochondria during apoptosis. They induce
the permeabilization of the mitochondrial membrane
with the release of cytochrome c and the formation of
the apoptosome [38]. The major pro-apoptotic protein
Bax ... mitochondria
and then translocate to the nucleus where they induce DNA fragmentation in a caspase-independent manner. Whether apoptosis is induced
or not depends on the...
... enthalpy is the sum of two
terms: the reaction enthalpy, independent of the buffer
used in the experiment, and another term representing
the contribution of the proton ionization of the buffer,
which ... HCl, 1 mM MgCl
2
, 100 mM NaCl. The
upper panels show the raw data for the
heat effect during the titrations; the lower
panels are the binding isotherms.
Table 2. Th...
... another factor or other
signals are required to fully derepress Meis2d.
The Meis2e splice variant retains the Hth domain,
but lacks both the HD and the AD. It could therefore
interact with Pbx, but ... lines.
It may therefore represent a significant proportion of
the functional Meis3 protein. However, further work
will be required to determine the relative levels of the
protein...
... We
prepared the 46-nucleotide DNAzyme with DNA-cleaving activity
(PL DNAzyme), and then grafted the deoxyribonucleotide residues from
an 8–17 variant DNAzyme into the region of stem–loop I and the catalytic
core ... ssDNA
[21]. The 5¢-arm of the enzyme binds the cleavable
sequence via Watson–Crick base pairs and the 3¢-arm
through formation of a DNA triplex.
The catalytic...
... agreement with the results of the NMR.
The addition of SDS to the C-peptide sample leads to
formation of larger objects, even at an SDS concentra-
tion of only 500 lm, which is well below the CMC.
The ... about the calcu-
lated population distributions cannot be made. The
DLS experiments, however, do confirm the formation
of C-peptide oligomers in the presence of SDS. The...