Tài liệu Báo cáo khoa học: Limited suppression of the interf

Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx

... described in Fig. 6C. The black and white arrowheads indicate Cardif and the cleaved Cardif, respectively. (B) Cardif is cleaved by NS3-4A in the cured Oc cells. The Oc cells were cotransfected with the ... FEBS Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes Hiromichi Dansako...

Ngày tải lên : 18/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... metal cofactor, respectively. SODs are grouped into four classes according to their metal cofactors: copper and zinc-containing SOD (Cu,Zn-SOD), iron-containing SOD (Fe-SOD), manganese-containing ... elucidated. In the present study, for the ﬁrst time, we describe the crystal structure of ApeSOD. In particular, we focus on the coordination of the metal cofactor in the ac...

Ngày tải lên : 14/02/2014, 22:20

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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... collected in the order: substrate (cytidine coeluting with nonreacted CDP), product (deoxycytidine) and by- product (cytosine) for analysis by liquid scintillation counting (Wallac 1410; Pharmacia, ... & Auling G (1998) Clon- ing and sequencing of the nrdF gene of Corynebacterium ammoniagenes ATCC 6872 encoding the functional metallo-cofactor of the manganese-ribonucleo...

Ngày tải lên : 15/02/2014, 01:20

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Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf

... the ACT1 domain is inserted into the ACT2 domain; and (c) two ACT domains, each from a different chain, interact to form an effector-binding unit. The effector-binding unit of the ACT domain ... dimers in the asymmetric unit. A chain, magenta; B chain, yellow; C chain, cyan; D chain, green; E chain, brown; F chain, blue. (B) EF chain dimer. Crystal structures of regulatory...

Ngày tải lên : 18/02/2014, 08:20

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Tài liệu Báo cáo khoa học: Identiﬁcation of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc

... CTC GGA TCC CTT TGA CAT GGC TTG AAG (lpxX antisense; BamHI site underlined) This study b1E1 CTC GAA TTC GTA TCA TAC TGC CCG ACC (inserting zeo gene; EcoRI site underlined) This study b1E2 CTC GAA ... study zeo EcoRI-2 CTC GAA TTC TCA GTC CTG CTC CTC GGC (zeocin antisense; EcoRI site underlined) This study b2SP GAG TTG CCA TCA TCA GCA (lpxL sense) This study b2AP AAT TGG TGT CAT CGG CTT (lpxL...

Ngày tải lên : 18/02/2014, 14:20

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Tài liệu Báo cáo khoa học: Kinetic characterization of the ﬁrst step of the ribozyme-catalyzed trans excision-splicing reaction docx

... self- splicing with adenosine: evidence for a single nucleo- side-binding site. Science 252, 434–437. 59 Golden BL & Cech TR (1996) Conformational switches involved in orchestrating the successive ... Molecular recognition properties of IGS-medi- ated reactions catalyzed by a Pneumocystis carinii group I intron. Nucleic Acids Res 31, 1921–1934. Kinetics of the trans excision-s...

Ngày tải lên : 18/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... A ˚ . The electron density of the whole main chain of the catalytic domain (B-chain) of DESC1 is well defined. Only a few side chains on the surface of the molecule are partially undefined in the ... the design of speci c inhibitors. To the west, the pocket is limited by Lys224 side chain. The flexibility of this side chain is reduced Crystal structure o...

Ngày tải lên : 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... solution which contains 100 mm CH 3 COOH ⁄ CH 3 COONa as buffer. This relatively high concentration justiﬁes the presence of acetate in the crystal structure. The binding of acetate in the active ... role in the enzymatic reaction which will be discussed later. The structure of the active site, the type of protein ligands, the zinc-binding motif, the presen...

Ngày tải lên : 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... PSII centers are inhibited for Q B reduction by binding of a DCMU molecule in the Q B pocket; the remaining PSII centers are unaffected. However, a saturating concentration of DCMU would inhibit ... 4773 of Q A – in PSII centers that are affected by the nonsatu- rating concentration of inhibitor [7,8]. The E m of Q A is raised in the presence of DCMU in...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khoa học: An investigation of the substrate speciﬁcity of the xyloglucanase Cel74A from Hypocrea jecorina pdf

... to restrict binding of a branched Glc residue [14]. Docking experiments on OXG-RCBH indicate that the shorter loop length in Cel74A can be expected to increase access of an oligo- saccharide to ... a circular plasmid carrying the catalytic domain of Cel7A (carbohydrate-binding module I) behind the cel7a promoter. The resultant strain was grown at 25 C in a batch-fed proce...

Ngày tải lên : 19/02/2014, 05:20

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