... with unknown structure. We report here the three-dimensional
structure of the At3g16450.1 protein from Arabidopsis, which consists of
two tandem repeats. Because the size of the protein is larger ... for the sugar structure, although none of the
various sugars tested exhibited a strong affinity.
Discussion
In this study, we determined the solution structure...
... constraint of 4.5 A
˚
between the
geometrical center of the main chain nitrogen of Arg42 and
O6 of the leading guanine. Accuracy of the docking was
assessed on the basis of scoring values calculated by ... the positions of the ribose and base of the
second nucleotide. In all cases, the base of the second
nucleotide interacted with the Asp66–Thr69 loop...
... CG2,
the lack of clear homology outside these regions, and the
unique tripeptidase speci®city of peptidase T, suggested that
the structure of the two enzymes would in part differ. We
report the ... while the Ne2 atom is hydrogen
bonded to a n aspartic acid residue, albeit from a different
part of the structure. The role of the t hreonine residue in the
HXDT...
... as the ATP-unbound and Ca
2+
-bound
E1Ca
2+
form. In the histogram (Fig. 2A) of the dis-
tribution of the height of the projection of the embed-
ded molecule above the flat membrane surface, the
average ... Histograms of the height differences between the top of
SERCA and the surface of the membrane. Statistical section analy-
ses of SERCA were performed...
... length
restrictions of this minireview. The work of S.W. was
supported by the Deutsche Forschungsgemeinschaft
(We2843 ⁄ 2 -1) and BGAG-Stiftung Walter Hesselbach.
The work of S.B. is supported by grants from the ... to the formation of VirB2-VirB5 complexes followed by T-pilus assembly. The assem-
bled T4SS then triggers the secretion of substrates from the...
... for a therapeutic approach in AD [13–15].
Protein structure and gene
organization of ADAM10
The enzyme ADAM10 belongs to the subgroup of
metzincins within the zinc proteinases family. The typi-
cal ... point of intervention within the biosynthetic
pathway of ADAM10 is provided by directly interfer-
ing with the expression of the gene for ADAM10: the
promoter re...
... preferentially
the phosphorylation of the p46 isoform of JNK,
whereas sorbitol treatment induced phosphorylation of
both the p46 and p55 isoforms. To confirm functional
activation of the JNK signaling ... efforts were frustrated by the lack of specificity
of SP600125, which has also been reported by others
[42,43]. Resolution of the importance of JNK activa-
tion...
... induce
the synthesis of HSPs that act as molecular chaperones
through binding to the hydrophobic surfaces of
unfolded proteins, thereby facilitating refolding of pep-
tides and preventing protein ... development of the
lens and maintenance of the olfactory epithelium [102].
The first evidence for a developmental function of
HSF4 was provided by population genetic stud...
... the intermembrane space of yeast mitochon-
dria by a stop-transfer mechanism. Cell 69, 809–822.
20 Stuart R (2002) Insertion of proteins into the inner
membrane of mitochondria: the role of the ... inner
membrane proteins [14].
The mitochondrial targeting signal (MTS) of pro-
teins directed to the IMM is recognized by receptors
of the translocase of the outer...
... and to mea-
sure the mobility of the components in the PGCs. The
medaka embryo was peeled off the chorion, and the
segment containing the part of PGCs was excised for
observation by microscopy and ... processes of replace-
ment of molecules in granules from other parts of the
same granules or from the cytosol. We performed a
bleaching experiment in a whole pa...