Tài liệu Báo cáo khoa học " ĐÁNH GIÁ KHẢ NĂNG THÔNG HƯỚNG KH

Tài liệu Báo cáo khoa học " ĐÁNH GIÁ KHẢ NĂNG THÔNG HƯỚNG KHI THI CÔNG ĐƯỜNG HẦM BẰNG PHƯƠNG PHÁP ĐÀO HẦM ĐỐI HƯỚNG " pptx

... ĐÁNH GIÁ KHẢ NĂNG THÔNG HƯỚNG KHI THI CÔNG ĐƯỜNG HẦM BẰNG PHƯƠNG PHÁP ĐÀO HẦM ĐỐI HƯỚNG KS. DIÊM CÔNG HUY Viện KHCN Xây dựng Tóm tắt: Trong quy trình thi công đường hầm thì công ... phát hiện. Để đánh giá được khả năng thông hướng của các hướng khi thi công bằng phương pháp đào hầm đối hướng trước hết phải kiểm tra độc lập độ ổn định của...

Ngày tải lên : 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... four times in PBSCM before elution with 50 lLof20mm dithioerythreitol-containing SDS sample buffer, and incubated for 30 min at 37 °C. The dithioerythreitol reduces the disulﬁde bridge in the ... hypothesized to play a major role in this selective loss of dopaminergic neurons, and the cyto- solic fraction of DA is considered particularly toxic [3,7,26]. This highlights the importance of ......

Ngày tải lên : 14/02/2014, 21:20

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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

... mutagenesis performed in this study. This supplementary material can be found in the online version of this article. Please note: As a service to our authors and readers, this journal provides supporting ... GST–IST1 L326D,L353A was induced with 0.5 mm isopropyl thio-b-d-galactoside for 3 h at 30 °C, and the proteins were puriﬁed by binding to glutathione–Sepharose 4B beads according to the m...

Ngày tải lên : 18/02/2014, 04:20

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Tài liệu Báo cáo khoa học: The stereochemistry of benzo[a]pyrene-2¢-deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases pptx

... to changes in the microenvironment when this adduct is flanked by a target cytosine that undergoes flipping in the MTase– DNA complexes. In accordance with this, the depend- ence of the fluorescence ... prokaryotic MTases M.HhaI and M.SssI does not have any significant effect on DNA methylation rates. This difference may be associated with the intercalative conformation of the (+)-cis adduct and...

Ngày tải lên : 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Changes in purine speciﬁcity in tandem GAF chimeras from cyanobacterial cyaB1 adenylate cyclase and rat phosphodiesterase 2 pptx

... construct III (3.3 lm ). This indicated that the L259V mutation not only reduced cGMP potency but also interfered with cNMP binding and signalling in general. Apparently, this amino-acid side chain ... has an amino-acid sequence identi- cal with the mouse isoform in this region, and the GAF tandem domain from the cyaB1 AC because this domain, despite its sequence identity with that of cyaB2 (...

Ngày tải lên : 19/02/2014, 02:20

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Tài liệu Báo cáo khoa học: Rac upregulates tissue inhibitor of metalloproteinase-1 expression by redox-dependent activation of extracellular signal-regulated kinase signaling pptx

... TIMP-1 promoter in HepG2 cells and that this effect is mediated by Rac [33]. To identify the regulatory elements within the TIMP-1 promoter that mediate this effect, we ﬁrst tested different length ... buffer (pH 7.5), containing 150 mm NaCl, 10 mm MgCl 2 , 0.5 mm dithiothreitol, 15 lm ATP, 5 lCi [c- 32 P]ATP, and 1 mgÆmL )1 glutathione S-transferase (GST)–cJun (1–79; Alexis, Gruenberg, Ger...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx

... that this residue is not necessary for the activity of RTA. This is supported by the earlier observation showing that deletion of residues 258–262 did not inactivate RTA [30]. However, although this ... Unlike this tetranucleotide, the sequence of the stem does not appear to be important as long as it is at least three base-pairs in length [8]. Interactions between RTA and the rRNA backbone i...

Ngày tải lên : 19/02/2014, 12:20

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Tài liệu Báo cáo khóa học: Trichostatin A reduces hormone-induced transcription of the MMTV promoter and has pleiotropic effects on its chromatin structure pptx

... (A–F) cover this region [4], one of which, nucleosome B, covers the DNA segment around position )60 to )240. This segment contains four glucocorticoid response elements (GREs) [4–6]. This whole ... 73–84. 34. Vitolo, J.M., Thiriet, C. & Hayes, J.J. (2000) The H3–H4 N-ter- minal tail domains are the primary mediators of transcription factor IIIA access to 5S DNA within a nucleosome. Mol. ....

Ngày tải lên : 19/02/2014, 12:20

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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... receptor (thick lines) in nonpermeabilized cells cultured without and with iron. Thin lines represent background staining with mouse Igs. (B) Histograms show background staining with no antibody (thin ... with rab- bit Igs (thick lines) as ﬁrst step in permeabilized cells. (C) Histo- grams show ferritin expression in nonpermeabilized (thin lines, – saponin) and permeabilized cells (thick line...

Ngày tải lên : 19/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx

... relaxa- tion of the solvent water bulk. This is at difference with Fe(III)heme, due to the different afﬁnity of the metals for phenolic oxygen ligands [6,8]. Nevertheless, this contribution is not evident ... essentially be regarded as relative domain rearrangements to the I-II and II-III inter- faces. This allosteric regulation is not observed for short FAs (e.g. octanoate) that preferably b...

Ngày tải lên : 20/02/2014, 02:22

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