... Author Journal compilation ª 2009 FEBS
Electrostatic contacts in the activator protein-1 coiled coil
enhance stability predominantly by decreasing the
unfolding rate
Jody M. Mason
Department of Biological ... 2009)
doi:10.1111/j.1742-4658.2009.07440.x
The hypothesis is tested that Jun–Fos activator protein-1 coiled coil inter-
actions are dominated du...
... the luminal membrane of
endothelial cells via interactions between selectins,
chemokines and integrins. After docking to the vessel
wall, leukocytes extend their processes toward interen-
dothelial ... and
differences in the luminal–abluminal polarity of the
endothelium. To bridge the gap between in vitro condi-
tions and the actual environment, experiments should
incorporate...
... with
increasing solvent-accessibility of the His210 side-chain. In
the protein interior, the dielectrostatic constant is lower than
on the protein surface, while the dielectrostatic constant in
water ... depending on NaCl concentration
were determined as part of the investigation into the
shielding from solvent of the electrostatic interaction
between Asp113 and His210....
... lacking p15
INK4b
are
resistant to the growth-inhibitory effects of gefitinib.
As the level of p15
INK4b
is increased by MEK inhibi-
tors, but not by Akt inhibitors, the induction of
Fig. 3. The ... cyclin-dependent kinases (CDKs), in asso-
ciation with each other, play key roles in promoting
the G
1
-to-S phase transition of the cell-cycle by phos-
phorylating the reti...
... that induction of FRET does not sim-
ply depend on the length of linkers. In the linker con-
taining seven amino acids, a Pro-Pro sequence that is
not contained in other linkers is incorporated. ... decreased the
lifetimes of cerulean–GAPDH in the cytoplasm, con-
firming the results shown in Fig. 2B,D. The lifetimes
analyzed by the two-exponential fitting are summarized...
... B, Finet S &
Longhi S (2005) The intrinsically disordered C-terminal
domain of the measles virus nucleoprotein interacts with
the C-terminal domain of the phosphoprotein via two
distinct ... (2008) Mapping
alpha-helical induced folding within the intrinsically dis-
ordered C-terminal domain of the measles virus nucleo-
protein by site-directed spin-labeling EPR spectroscopy....
... plateau in the spinal cord about
10 days after birth (Fig. 5E).
To determine the origin of AChE in the spinal
cord, the lumbar region of the spinal cord was
sectioned and stained with the PRiMA ... be analyzed by real-time PCR. The PRiMA I pro-
tein level in the spinal cord, determined in western blots
with the PRiMA antibody (recognizing the cytoplasmic
domain o...
... lacks other aromatic
rings in the vicinity. In comparing the three structures, it
should be noted that the residue numbering is the same
for the yeast enzyme and Pf TIM, but is increased by 1
in ... is not regained even
upon dimerization. Thus, position 74 is not only critical
for the stability of the dimer, it may also be involved in
maintaining the integrity of...
... the brain, is regulated by the VEGF system
in combination with other signaling systems such as
the angiopoietin–Tie, ephrin–Eph, Delta–Notch sys-
tems, and the Wnt pathway.
Furthermore, the blood ... clinical outcomes
by developing new strategies with combination therapy using known anti-
angiogenic drugs or by developing unique medicines specifically targeting
the blood ve...
... angiogenesis in the brain. Is it
possible that some of the acute neurovascular events in
the brain after stroke represent an endogenous attempt
by the brain to prepare the substrates necessary ... signaling actions involving
the platelet-derived growth factor and low-density lipo-
protein receptors [32,33]. These experimental findings
are now beginning to be supported by cl...