... Research Center Borstel. Journal compilation ª 2008 FEBS 659
Regulators of G-protein signalling are modulated by
bacterial lipopeptides and lipopolysaccharide
Sabine Riekenberg
1
, Katja Farhat
1
, ... or TLR2 ⁄ 6 lipopeptide ligands and the
TLR4 ⁄ MD2 ligand lipopolysaccharide, microarray analyses show only
modulation of RGS1 and RGS2 among all the regulators...
... 5-methyltetrahydrofolate-binding pocket of rat liver MetS. Atoms within 7 A
˚
of the docked ligand are shown:
hydrophobic amino acid residues are coloured bronze, and hydrophilic acid residues are coloured ... result of this approach indicated the orienta-
tion of 5-methyltetrahydrofolate in the MetS active
site. Figure 5 shows the superimposed structures of
DHPS and 5-met...
... mediated by the ANT in maximally
polarized (forward mode of both ANT and ATPase)
and maximally depolarized (reverse mode of both
ANT and ATPase) mitochondria.
Flux control coefficients of ANT and F
0
F
1
-ATP
synthase ... difference of DWm (termed
Delta phi) before and after the addition of ADP
(2 mm) to WT and CYPD KO mitochondria, and cal-
culated on the basis of...
... University of Science and Technology, Hunan, China
3 College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, China
4 School of Science, ... activation of endothelial
cells and macrophages, as well as the differentiation
and proliferation of VSMCs. Overexpression of KLF4
induced expression of multiple anti-inflamma...
... cuvette closed by
a Teflon stopper. The oxygen was consumed by most
substrates within the mixing time as revealed by the
immediate bleaching of the TPQ 500 nm band and by the
appearance of absorption ... the absence of H
2
O
2
,
a specific inactivating reaction, without formation of the
350 nm band, was induced by some aldehydes, notably
putrescine. Some mechanisms of inac...
... observation of species of
increasing mass spaced by 162 Da reflects the presence of
glycoforms with an increasing number of hexose (probably
mannose) units. Phosphorylation is indicated by the pres-
ence ... results of detailed carbohydrate analysis (Fig. 6) and
peptide MS experiments (Fig. 3A) define the glycoform
structure and the sites of glycan attachment in the present...
... Inhibition
of the ATPase activity was then explained by a slowing
down of the formation of active complex in solution. On the
other hand, the acceleration of the sliding velocity (and the
lower number of ... proteins as revealed by
the absence of cosedimentation of T800 with myosin during
a low speed centrifugation experiment (data not shown),
and by the absence of in...
... Àðk
A
=k
0
Þ=½Að1Þ
where k
A
and k
0
are the inactivation rate constants
either in the presence or in the absence of various
concentrations of a ligand A, k
2
and k
1
are the
rate constants of the inhibition of the ... Sigma-Aldrich and Carlo Erba (Milan, Italy), and
were of the highest available grade.
Acknowledgements
Thanks are due to Prof G.L. Sottocasa and Dr...
... in its
1
D
g
state;
1
O
2
)is
generated by a number of enzymatic and chemical reactions,
by UV exposure, and by visible light in the presence of a
number of exogenous or endogenous cellular sensitisers.
1
O
2
generation ... inactivation of
GAPDH and GR occurs via oxidation of nonactive site
residues, that bring about loss of functional integrity, the
stoichiometry of...
... morphologically and
biochemically distinct modes, necrosis and apoptosis [1].
Necrosis is characterized by swelling of organelles and cells,
followed by lysis of the plasma membrane and random
DNA ... (Hsps) are a set of highly conserved
proteins that are induced in response to physiological and
environmental stress, and are classified into several families
on the b...