Tài liệu Báo cáo khoa học: Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses pdf
... localized
fashion as revealed by mutational and X-ray
crystallographic analyses
Muhammad S. Rohman
1
, Takashi Tadokoro
1
, Clement Angkawidjaja
1
, Yumi Abe
1
,
Hiroyoshi Matsumura
2,3
, Yuichi ... 4· -RNase HI, 5· -RNase HI
and 6· -RNase HI proteins against thermal denatur-
ation were analysed as described for R97G -RNase HI
and D136H -RNase HI. Therma...
... heat shock transcription
factor 1 deficiency alters cardiac redox homeostasis
and increases mitochondrial oxidative damage. EMBO
J 21, 5164–5172.
36 Yan LJ, Rajasekaran NS, Sathyanarayanan S & ... asso-
ciated with constitutive DNA-binding activity, which
was detected in a band-shift assay of either HSF1 or
HSF4. One could imagine that such a high expression
of HSFs, accompanie...
... 3¢-GTCACAGAGCCACAGTTCCAG
CCAGGA-5¢. Codon 305 of C-YES was mutated from
AAA (Lys) to AGA (Arg) with the forward primer: 3¢-GG
AACCACGAAAGTAG CAATC AGA ACACTA AAACC A
GGTACAATGATGC-5 ¢ . All vector inserts ... resulted in a concomitant reduction
in kinase activity as measured by both plate and tube
assays. The accumulation of C-YES in the precipitates
correlated with the ap...
... from
bacteria and transferred to a new Petri dish and incu-
bated overnight with medium containing gentamicin.
Bacterial growth of each strain was assessed by viable
counts, both during initial infection ... SD)M
419
AAAA
428
[14]
5. GST-ExoS(LDL426–428AAA) S
419
QGLLDAAAA
428
This study
6. GST-ExoS(DALDL424–428AAAAA) S
419
QGLLAAAAA
428
This study
7. GST-ExoS(D42 4A; D42 7A) S
419...
... investi-
gated the presence of bilitranslocase in carnation
petals and found a BSP uptake, inhibited by anti-
bodies against bilitranslocase, in microsomal, plasma
membrane and tonoplast vesicle fractions. ... FEBS
Characterization of electrogenic bromosulfophthalein
transport in carnation petal microsomes and its inhibition
by antibodies against bilitranslocase
Sabina P...
... for
reaction against HydA1 and HydA2 overexpressed in
E. coli and against HydA2 in anaerobically induced algal
extracts.
Algal cells were harvested during the mid-logarithmic
phase by centrifugation ... (5¢-AACATCTTCAAGGA
GCGTGGCATC-3¢)andBE3P1(5¢-AGACAGCAGGA
GACTCACAATCAC-3¢), were used to amplify a C. rein-
hardtii expressed sequence tag (EST), BE337478, from a
strain 21gr cDN...
... DMNH
2
ðdÞ
DMNH
2
þ Fumarate ! DMN þ Succinate ðeÞ
The substrate sites of hydrogenase and of formate
dehydrogenase are exposed to the bacterial periplasm,
whereas that of fumarate reductase faces the cytoplasm
(Fig. ... hydrogenase measured with DMN as
acceptor w as ne arly the same i n t he different preparations
(Fig. 2 A) . In contrast, the activity of fumarate reductase
(...
... formal device of
functional un-
certainty, as
defined by Kaplan and Zaenan [3]
and Kaplan and Maxwell [2]. In this paper, we
relate this characterization to that provided by
Tree ~,djoining ... level). In [10], we have formal-
ized this notion by introducing graph adjoining
grammars which generate exactly the same lan-
guages as TAGs. In a graph adjoining gra...
... }
Retrieval of Bilingual
Article Pair
WWW
(News Sites)
Effect of Cross-Language IR in Bilingual Lexicon Acquisition
from Comparable Corpora
Takehito Utsuro
Takashi Horiuchi
and
Kohei Hino
Graduate ... idea that semantically
similar words appear in similar contexts (Fung,
1995; Rapp, 1995; Kaji and Aizono, 1996;
Tanaka and Iwasaki, 1996; Fung and Yee, 1998;
Rapp, 1999; Tanak...
... 389–400.
7 Ebashi S & Kodama A (1965) A new protein factor
promoting aggregation of tropomyosin. J Biochem 58,
107–108.
8 Yokokawa M, Wada C, Ando T, Sakai N, Yagi A,
Yoshimura SH & Takeyasu K ... of both ATP and Ca
2+
was
measured in the single line FSAFM images, and
Fig. 1. Single-molecule imaging of SERCA dynamics in the presence of nucleotide and Ca
2+
. (A...