Tài liệu Báo cáo khoa học: Molecular and cellular specificity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx
... (vitellogenesis inhibiting hormone) and CHH
(crustacean hyperglycemic hormone) of the crustacean
have the same precursor? Immunolocalization of VIH
and CHH in the X-organ sinus gland complex of the
lobster ... axon terminal in
the sinus gland, as a result of late and progressive
isomerization of the Phe3 of the CHH during the
migration of the...
... cell in ltrates of the lung and fibro-
sis in the liver [110].
Conclusions
The novel members, PDGF-C and -D, of the PDGF
subfamily of the cystine knot family of growth factors
are potent cytokines ... on their
activities points to a wide scope of biological effects;
however further understanding of how these factors
interplay with other members of the cystine...
... implicated at
the nucleotide binding domains and the regulatory
domain, indicating the importance of these domains for
the tetramerization of the enzyme [18]. It was shown that
serine binding induces ... pathways. In addition to its role in the synthesis
of
L
-cysteine and
L
-glycine and also in the formation of
L
-methionine by the interconversion of
L
-...
... different. The results of
Southern blotting also indicated the AK2 as a single copy
gene in L. donovani. Investigation of transcription of the AK
gene by Northern blot analysis during the Leishmania ... acceptor (NMP),
whereas the LID-domain prevents the hydrolysis of the
Mg-bound phosphoryl donor in the active site. The relative
movement of the NMP-binding s...
... substrate. The site-directed
mutants in this study were designed in order to
confirm the functional importance of D31 and L32
as substrate-binding, cofactor-binding and inhibitor-
binding sites.
The ... mutant V76A, and the MH831 par-
ent strain MG1655. All strains grew in the presence of thymidine
(data not shown). Only the strains plotted in the figure grew in...
... Scheme of the three-hybrid strategy used in
the screen. (B) Number of colonies identified in each step of the
screen. (C) Distribution of the 63 distinct sequences in relation of
their position in the ... identified
for each APUM in the
PFAM analysis. Gray
circles represent the localization of repeats
in the protein and the numbers indicate the
posi...
... analysis of substrate on the active site
of HYD
Js
The substrate binding pocket in the active site was inves-
tigated based on the homology model to obtain more
information on substrate binding in ... (B) The SGLs of HYD
Bp
are shown as magenta
lines and those of HYD
Js
are shown in green. The transition state
of
D-p-HPH is shown in turquoise, and the four...
... stages of
development. The amounts of pro-b-conglycinin and
proglycinin are considered to be nearly equivalent to
the synthesis levels of both b-conglycinin and glycinin,
as pro-b-conglycinin and ... proglycinin are transient pro-
tein forms that are present in the ER prior to process-
ing in the protein storage vacuoles. The synthesis of
proglycinin and pro-b-co...
... Second,
the building block of the ligand having the strongest
contact with the protein shows the most intense NMR
signals, enabling mapping of the ligand’s binding epi-
tope. Finally, and most ... could indicate that the corre-
sponding hydroxyl groups are involved in protein
binding.
The results for the linebroadening measurements of
protons H1a in the alpha...
... and 80 °C between 190 and
260 nm with a step increase of 0.2 nm, and a bandwidth of
1 nm. Thermal stability of Rep245 was measured by incu-
bating the protein at 60, 70 and 80 °C for 30 min and ... account for the promiscuous nature of
the synthesis functions performed by the prim–pol
domain and to discriminate between the functions of
in vitro primase and...