... siRNAs versus siRNA 396
was significant at P < 0.05.
A
B
Fig. 5. Effect of terminal and internal mismatches on siRNA TRBP
and siRNA Dicer complexes. (A) EMSA of siRNA TRBP and
siRNA Dicer complexes ... function of TRBP in RNAi.
Here, we have characterized the interactions of
siRNAs that contain terminal mismatches with TRBP
and Dicer, and determi...
... mm enhanced the
binding of InsP
6
to PhyAsr (Table 1). Given the absence
of structural changes as a function of ionic strength and
ligand binding, the effect of ionic strength on catalytic
activity ... absence
of ligand, the WPD loop of PTP1B and RPTPa adopts
an open (inactive) conformation, and upon ligand bind-
ing, it adopts a closed (active) conformation (Fig. 3A)...
... role of this region in the transcription of the
Ig-b gene. Deletion of region III decreased the expres-
sion of Ig-b mRNA to 40% of the expression in prede-
letion cells (Fig. 4A). Region III ... hybridization. Position of the probes, the size and position of restriction fragments hybridizable with the probe, and exon ⁄ introns are
shown as in Fig. 2A. Positions of the DT4...
... cation binding site has been des-
cribed so far. As the regulatory effect of the trans
calcium concentration was voltage-dependent and
changed as the function of the cis EGTA concentra-
tion, ... several cytoplasmic ligands. It is activa-
ted by millimolar concentrations of ATP and micromo-
lar Ca
2+
, and inhibited by higher concentrations
(> 100 lm)ofCa
2+
and by millim...
... analyzer. Stock solutions and dilutions were
made in distilled water and the peptide concentrations were
determined by amino-acid analysis.
Construction and purification of HIV-1 PR mutants
HIV-1 ... the
substrate binding site of the PR, and these mutations
have considerable impact on PR activity and specificity.
Other resistant mutations alter residues outside of the
substr...
... term
correspondences are estimated, in terms
of both computational complexity and
the performance of precise estimation of
bilingual term correspondences.
1 Introduction
Translation knowledge acquisition ... situ-
ation. One limitation of the corpus-based trans-
lation knowledge acquisition approach is that the
techniques of translation knowledge acquisition
heavily rely on avai...
... a
function of dextran concentration, the activity of
GSGB was highly dependent on dextran (filled circles
in Fig. 2B); however, that of GS was nearly zero, inde-
pendently of dextran concentration ... reciprocal plot of nigerotriose accep-
tor reaction of GS.
Fig. S4. Separation of nigerooligosaccharides by acti-
vated carbon chromatography.
Doc. S1. Simulation of the glucan...
... The formation of pyroglutamic acid moieties from Glu
and Gln were considered as variable modifications.
Moreover, automated de novo sequencing of the nano-
ESI–qTOF and MALDI-TOF ⁄ TOF MS data ... com-
ponents of the extracellular matrix (ECM). MMPs
play a central role in connective tissue remodeling pro-
cesses and regulation of cell matrix composition
through their effects on...
... demonstrate strong modulation of
expression after stimulation with LPS and LP. LP and
LPS mediate immunomodulating functions, at least
in part, through regulation of RGS1 and RGS2
expression.
Results
RGS1 ... RGS1 and RGS2 mRNA and modu-
late expression after stimulation with LP and LPS.
Expression patterns of RGS1 and RGS2 after
activation of TLR3 and TLR9
To investig...
... this, UV-irradiation of the cells (2.4 JÆm
)2
), although
inducing a strong activation of JNK1/2 and only a very
weak phosphorylation of p38, had no effects on C TGF
expression, which ruled out ... expression
occurs via increased transcription and/ or by stabilization of
the CTGF transcripts and t he role of RhoA GTPase and
p38 signaling in such a regulation, nuclear run -o...