... that
includes a DNA-binding domain, a ligand-binding
domain and a transactivation domain. The DNA-bind-
ing domain is responsible for DNA binding specificity
and dimerization, and the ligand-binding ... ª 2010 FEBS
MINIREVIEW
Mixed lineage leukemia: roles in gene expression, hormone
signaling and mRNA processing
Khairul I. Ansari and Subhrangsu S. Mandal
Departm...
... proteins. Thus, the MEN1 ⁄ LEDGF-interacting
domain linked to DNA-binding domains (AT-hook and
MT domain) becomes disconnected from the PHD
domains, the FYRN domain, the transactivating
domain, ... different signaling pathways,
for example, protein kinase C, protein kinase A,
RAS ⁄ RAF, WNT-, phosphatidylinositol 3-kinase and
Hedgehog, and thus affects metabolism, the cell cycle,
gen...
... S-adenosyl-homocysteine; BD, bromo domain; CBP, CREB-binding protein; CREB, cAMP response element-binding;
H3K4, histone H3 lysine 4; HMT, histone methyltransferase; MLL1, mixed lineage leukemia protein-1; ... required
for the structural integrity of the protein, as mutation of
any of the cysteine residues involved in zinc coordina-
tion results in protein unfolding [22]. The structur...
... binding profiles in a time-dependent
manner in ERE1 and ERE2 (Fig. 5B). In agreement
with the above findings, binding of ERa and ERb was
increased in both ERE1 and ERE2 in the presence of
E2. Interestingly, ... Binding of ERa and ERb
was increased in both ERE1 and ERE2 of the
HOXC13 promoter (Fig. 5A, lanes 1–4). The levels of
E2-induced binding of ERa and ERb were highe...
... Prelamin A and BAF are also binding
partners of emerin [39]. Interestingly, the N-terminus
of human MAN1 binds the human emerin itself
(Fig. 1; [8]). Emerin is an integral membrane protein
and ... for
binding to emerin in vitro. J Biol Chem 278, 6969–6975.
8 Mansharamani M & Wilson KL (2005) Nuclear mem-
brane protein MAN1: Direct binding to emerin in vitro
and two modes of bind...
... con-
taining DNA spots (5 lg each) corresponding to inter-
nal fragments of ADC or cruzipain genes. The latter
(used as a control) is a housekeeping gene that encodes
the main cysteine proteinase ... Analysis of a cDNA-
encoding arginine decarboxylase from oat reveals simi-
larity to the Escherichia coli arginine decarboxylse and
evidence of protein processing. Mol Gen Genet 224,
431–436...
... enzyme–inhib-
itor binding interaction.
log K
ðappÞ
i
¼ log K
Ã
þ 1: 18Z
E
Z
L
ffiffi
I
p
ð7Þ
Discussion
In this study we have determined that neomycin B and
its close structural analog, neamine, are mixed- type
noncompetitive ... manifes-
ted in Figs 3 and 4. Note that the Lineweaver–Burk
plot in Fig. 4 is distinctly nonlinear.
The results are illustrated in Fig. 5, in which the
w...
... shown that interactions between
KCs and endotoxin comprise the initiating event lead-
ing to hepatotoxicity in liver injury, including endotox-
emia and ischemia ⁄ reperfusion injury [14]. In our
studies, ... factor-a and interleukin-6, and increased
hepatic mRNA levels of tumor necrosis factor-a and interleukin-6 were
decreased by depletion of KCs. Our findings suggest that ab...
... of opening
of the initial double strand and the annealing of the
new duplex finally results in either the replacement of
the original strand or the expulsion of the invading
strand, according to ... unwinding. High-resolution X-ray struc-
tures have given insights into the mechanism(s) of
DEAD-box helicases. The binding sites for double-
stranded RNA and ATP overlap, resulting in coupl...