Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
... Journal compilation ª 2010 FEBS
X-ray crystallographic and NMR studies of pantothenate
synthetase provide insights into the mechanism of
homotropic inhibition by pantoate
Kalyan Sundar Chakrabarti*, ... basis for the homotropic inhibition of pantothenate synthe-
tase by the substrate pantoate was investigated by X-ray crystallography
an...
... mediated by other mammalian difla-
vin reductases and also subdomains of MSR; (b) the
extent of reductive remethylation of hMS catalysed by
the different redox states of MSR; and (c) the ability
of ... cob(II)alamin. Reduction of the Co centre
promotes the dissociation of the lower dimethylbenz-
imidazole base of the cofactor. Consistent with this,
the c...
... FTHF to the Gly binary
complex. The results highlight the role of E53 in bind-
ing of THF ⁄ FTHF and in the proper positioning of
l-Ser for direct attack by N5 of THF.
The structure of E53QbsSHMT ... held by hydrogen bonding interaction
with the phenolate oxygen of Y51, N of G257 and
NE2 of Q53 of the symmetry related subunit. Another
oxygen atom is hel...
... that the data were
described best by a two-binding site model. The IC
50
(the concentration of the competing ligand that inhib-
its 50% of the specific binding of [
125
I]TC-PCSK9) val-
ues for the ... are
assumed, then about 70% of the high-affinity sites and about 12%
of the low-affinity sites are expected to be occupied at the concen-
trations of unlabeled li...
... membrane, although the
nature of the cellular signal required and the mecha-
nism of its transduction are still a matter of debate
[14,15].
The third, major part of the T3SS is the ‘translo-
con’, ... mem-
brane-associated and faces the bacterial cytoplasm,
and is suggested to be involved in facilitating the entry
of export substrates into the secretion sys...
... expression of T-bet has been
seen within a wide variety of tissues and cells in the
Ginbuna crucian carp [27] and, in mammals, in the
lung tissue of mouse and spleen and thymus of human
and mouse ... CD4
+
CD25
+
Foxp3
+
T
reg
or whether
they induce the production of these cytokines from
another population of cells [20].
To date, our knowledge about the different...
... without fat, of WT and
H-PGDS TG mice (n = 6). Continuous
dissections of mouse fat and bone in the
whole body were quantified by use of a
micro-CT scanner and
LATHETA software
(Aloka). Open and closed ... obesity.
Experimental procedures
Generation of H-PGDS TG mice
The coding region of human H-PGDS was cloned into the
downstream sites of the chicken b-actin pro...
... Y5 and y6) that
vary in their binding profiles and in their distribution
in the central nervous system and periphery. Y1, Y2
and Y5 are the best characterized NPY receptors, and
the majority of ... stromal cells and osteoblasts [40,46–48],
despite the absence of the other Y receptors (Y2, Y4,
Y5 and y6) [40,46]. In addition to the presence of
NPY-immunoreactive...
... organelle and membrane proteins are synthe-
sized and folded with the assistance of molecular chap-
erones and other folding factors in the endoplasmic
reticulum (ER). In many cases, the process of ... synthesis of storage
proteins was initiated. These results suggest that GmPDIL-3a and
GmPDIL-3b may play important roles in the maturation of the cotyledon
by mechan...
... [20]. The role of SREBP-1a in the regulation of
cell growth and the cell cycle might be biphasic and
complex, and needs to be further investigated.
SREBP is evolutionarily conserved; however, the
key ... inhibit lipogenesis and lower
tissue and plasma triglyceride levels through inhibition
of SREBP-1c. The molecular mechanisms by which
PUFAs inhibit SREBP-1c are...