... role of miRNAs
with respect to the complicated regulation of the tran-
scriptome and signal transduction. Although miRNAs
and well-established cell signaling pathways have been
the subject of recent ... have focused
upon the role of miRNAs in regulatory network of
various cell signaling pathways. We summarize the
current knowledge of the interdependence of mi...
... with
binding of both MOF and Tip60 (equivalent to Esa1
in yeast NuA4) [124,130]. However, in human cells
depletion of MOF, but not Tip60, results in reduced
global levels of AcH4-K16 and defective ... recruitment of
MOF to recently transcribed regions, especially at the
3¢ ends of genes where H3-K36me3 is enriched [128].
In mammalian cells MOF also exists as part of a sepa-
ra...
... together, these findings may improve understanding of the cellu-
lar and molecular basis of the anti-inflammatory and paracrine effects of
mesenchymal cells.
Abbreviations
BrdU, 5-bromodeoxyuridine; ... important
mediator of the cells’ paracrine anti-fibrotic effects.
These findings help to improve our understanding of
the cellular and molecular basis of MSCs’ anti-inflam-
matory...
... advantage of p53 mutation analysis,
and a unique feature of this database, is the availabil-
ity of the residual activity of the majority of p53 mis-
sense mutants. The biological activity of mutant ... core
domain of the protein and were used for training and evalua-
tion of our prediction algorithm. Of the eight promoters, we
studied the WAF1 promoter in greatest det...
... M
r
values of the standards in thousands. Lanes 2–9, 5 lg of peak
P I, P II, P III ⁄ P IV and a mixture of other isoforms (*), respec-
tively, in the presence (lanes 2–5) or absence (lanes 6–9) of
2-mercaptoethanol. ... similarity of the A- and
B-chains of the four isoforms, a pairwise alignment
was performed and the values of identity expressed in
Fig. 3. (Continued).
Cha...
... Expression of Noxo1b, Noxo1c, Nox1, and Noxa1 in various human cell lines: androgen-independent prostate cancer cells
(LNCaP), androgen-independent prostate cancer cells (PC3 and DU145), and testicular ... Sciences, Turku, Finland).
Estimation of expression of cytosolic regulatory
proteins
Total cell lysates of transfected CHO cells were used to esti-
mate expression of No...
... Buchert
1
and Kristiina Kruus
1
1 VTT Technical Research Centre of Finland, Espoo, Finland
2 Protein Chemistry Research Group and Core Facility, Institute of Biotechnology, University of Helsinki, ... 7.3. Tyrosinase eluted at an NaCl con-
centration of 120 mm. Because of the high pI of
T. reesei TYR2, most of the Trichoderma cellulases
and hemicellulases could be separate...
... diamidino-2phenylindole
hydrochloride (DAPI) stained cells showing the nuc-
leus, and Fig. 7C, F, K and N are the merged images
of A and B, D and E, I and J, and L and M, respect-
ively.
Discussion
Nature ... stresses used for treatment of pea seedlings were cold (A and B), salinity (C and D),
wounding (E and F), drought (G and H), SA (I and J), ABA (K and L) an...
... existence of two
distinct HSF1 isoforms in rainbow trout and the formation
of heterotrimers of these isoforms in vitro.
Materials and methods
Cell culture and animals
Rainbow trout gonadal fibroblast cell ... 10.0;
GAPEXT
,0.05;
GAPDIST
,8;
MAXDIV
,
40;
ENDGAPS
,off;
NOPGAPS
,off;
NOHGAPS
,off.Graphical
output of the bootstrap figure was produced by the program
TREEVIEW
.
Isol...
... transcripts of these three APs are detected in all
seed cell types, in the outer cell layers of the anthers early in
flower development and in the guard cells of the sepals. The
mRNA of one of the ... by the prosegment, but also by
the 13 residues of the N-terminal of the mature enzyme and
by the ÔflapÕ. The anchorage of the prosegment and of part
of the N-terminus...