... The tandemly repeated domains of a b-propeller phytase
act synergistically to increase catalytic efficiency
Zhongyuan Li*, Huoqing Huang*, Peilong Yang, Tiezheng Yuan, Pengjun Shi, Junqi Zhao,
Kun ... domain and the functional
relationship of tandemly repeated domains in BPPs. We conjecture that
dual-domain BPPs have succeeded evolutionarily because they c...
... oxidative damage of chloroplastic
A
4
-GAPDH.
Results
Inactivation of A
4
-GAPDH by GSSG and other
oxidants, and protection by substrate and
cofactors
Incubation of recombinant Arabidopsis A
4
-GAPDH
with ... dithiothreitol. Activities are
given as a percentage of the initial activity measured before the
inactivation treatment.
M. Zaffagnini et al. Glutathionylation of c...
... (Chuck and
Young, 2004).
Computer-assisted tools that support manual
assessment based on checklists require natural
language understanding. Due to the complexity
of language, today’s natural language ... evaluation is difficult to fully auto-
mate. Manual fluidity evaluation relies on the
reader’s understanding of the text. It is therefore
superior to the automatic evaluation...
... for the activity of many sRNAs [11,15], it has
been proposed to be an RNA chaperone. The interaction
between Hfq and RNA may increase the propensity of
RNA to interact with itself or other RNAs, ... of Hfq has an apparent molecular mass of
50 ± 10 kDa, while the unfolded state of Hfq has an
average apparent molecular mass of 12 ± 5 kDa. This
indicates that the unfo...
... to abandon the idea of all-or-
none rules which was a central theoretical
assumption of these models compared to
connectionist ones. As the DRC model is largely
based on the interactive activation ... frequency of graphemic units in
the language and by the predictability of their
mapping to phonemes. We argue that these results
implicate the availability of...
... led to the degradation of the isolated
species [10]; as a consequence, the stoichiometry of the
complex was not established. The formation of stable
NrfHA complexes in the e-proteobacteria and ... subunit. The four NrfH hemes are all
in a low-spin state (S ¼ 1/2). One of them has a g
max
at 3.55,
characteristic of bis-histidinyl iron ligands in a noncoplanar...
... SDS/PAGE gave a single
band at apparent molecular mass of 34 kDa.
The binding affinity of the lectin in the hemolymph of
the freshwater crab, Paratelphusa jacquemontii, expressed
O-acetyl sialic acid ... Purification and characterization of a sialic acid specific lectin from
the hemolymph of the freshwater crab
Paratelphusa jacquemontii
Maghil Denis, P. D. Mercy Palatty...
... sequence that is
characteristic of cofactor-containing proteins translocated
into the periplasm via the Tat translocase [27]. As deduced
from the N-terminal sequence of AF499, the signal peptide
is ... typical a rchaeal
promoter elements. The sequence AAAGGTTAATATA
was f ound 64 bp upstream of the start codon of AF499; this
corresponds to the BRE element and the bo...
... name tank. Than %e the evaluatAon 5eat oemmon%y
used today For strafe.sial AntelIA|enee models.
A
more r~l;orous 5eat %e 5n Cry to
F~.5 a
mode/. 50 • emma
OF data.
?hAs ~e
the evaluate.on ...
"
One XntoreatAng ~Ant about 5h ~a ~rt~e~ar example %8
5hat ~t %e embedded wlthAn an "el~mlnatAon of
alternat£voo" arll~Nnt etruoture. The5 %a, 5hAm
"prqitAo...
... neutral residues can
retain HYD
Js
activity, and intriguingly, mutagenesis
of Leu92 to Ala and Val, two smaller hydrophobic
residues, actually reduced the catalytic activity to less
than approximately ... DNA
sequences flanking the Jannaschia sp. CCS1 HYD
Js
revealed an ORF encoding a putative allantoate amido-
hydrolase, which is part of the urate catabolic pathway
in many o...