... direction of transcription; the site ofthe transposon insertion identified in the O35E is denotedas EN:TN, and the location ofthe deletion replaced by the kanamy-cin-resistant gene (kanR) is between ... lipid-A andtwo phosphate moieties. The ions with m ⁄ z of 1519.6and 1541.6 are mono- and di-sodiated forms of the de-O-acylated LOS and the ion with m ⁄ z of 1479.6 is the anhydrous form ofthe ... mutant. MALDI-TOF-MS of de-O-acylated LOS from the mutant and glycosyl composition, linkage, and NMR analysis of its OS indicated that the LOS contained a truncated core OS and consisted of a...
... more interesting the idea of considering and analyzing the use ofthese devices. Another disadvantage isthe water consumption associated to the operation ofthese systems, whichis a scarce resource ... that air. The cooling capacity ofthe device depends on the amount of evaporated water fromthe humid surface into the air. Thus, the mechanisms of mass-transfer are consequence ofthe mass-diffusion ... Prof. Rey was member ofthe Eurotherm Committee, and currently is member of ATECYR and isthe head of Thermal Engineering Research Group of University of Valladolid. E-mail address: rey@eis.uva.es...
... days in whichthe LVTS was running. The participants in the sample consist of members ofthe LVTS and the Bank of Canada. For the purp oses of this study we exclude the Bank of Canadasince it ... mean ofthe current θs and a covariancematrix tuned so that the acceptance probability is approximately 25%-30%. The drawing procedure consists of simultaneously drawing the mean of the θs, which ... this is not quite true since the Bank of Canada is also a participant in the LVTS and acts as a drain of liquidityin Tranche 2. Specifically, the Bank of Canada receives payments on behalf of various...
... usingPAUPsoftware [23]. IFN-c was usedas an outgroup in this analysis.Expression ofthe IL-10 geneAnalysis of IL-10 gene expression in healthy tissues byRT–PCR. Total RNA extracted fromthe cell ... the IL-10 gene from carp has been isolatedand its genomic structure and expression analysis investi-gated. This work will pave the way for further investigation of the biological function of ... against products of b-actin. The x-axis indicates the time-periods of LPS incubation and the relative expression of the carp IL-10 gene on the y-axis. Controls for 0, 1, 3 and 6 h of incubation...
... (e.g., the height of a person), whilefor others it is only the center of a distribution(e.g., the weight of a coffee cup). Questionswith no trusted and exact answers were eliminated. From the ... tall is the tallest penguin? (122cm); What isthe height of a tennis net? (92cm); What isthe depth of the Nile river? (1000cm = 10 meters); How heavy is a cup of coffee? (360gr); How heavy is ... since the nature ofthe data is different from that of the QA dataset. Most ofthe questions asked over the Web target named entities like specific car brands,places and actors. There is usually...
... currenttopics, and persons in Japanese history. From these fifty terms, the system collected 610 terms in total; the average number of output terms per input is 12.2terms. We checked whether each ofthe ... number,hit, whichisan estimated number of pages that sat-isfy a given query. In case the query is a term, its hit is the number of pages that contain the term on the Web. We use the following ... notation.H(x)= the number of pages that contain the term x” The number H (x) can be used as an estimatedfrequency ofthe term x on the Web, i.e., on the hugest set of documents. Based on this number, wecan...
... observed at this period ofthe day. From 2 pm to 6 pm, the wind direction is oblique to the street axis and the wind speed is higher than in the morning. At these hours ofthe day, tracer ... in which, C isthe concentration of a particular pollutant in the street (g/m3 or mg/m3); E isthe emission ofthe pollutant from road traffic in the street; F is a function describing the ... were hexane, iso-pentane and 3-methylpentane. These three species account nearly to 60% of the total VOCs measured. The mean concentration of benzene registered in the 3/2 Street exceeds the...
... Cặmin)1. The thermal denaturation ofthese proteinswas fully reversible under this condition. The temperature of the midpoint ofthe transition, Tm, was calculated from curve fitting ofthe resultant ... of Tma-RNase HI.Likewise, the stability of Tma-W22A was not analyzedbecause the stability of Tma-W22A is not significantlychanged even when the HBD is completely removed,and therefore it is ... in a reversible manner. The ther-mal denaturation curves ofthese proteins are com-pared with one another in Fig. 8. The parameterscharacterizing the thermal denaturation ofthese pro-teins...
... addi-tion, the band at 1.0 GPa in Fig. 5C does notresemble the broad, featureless band typical of an unfolded protein. Taken together, these observationssuggest that the unfolding at the level ofthe ... grant from the technology foundation STW, the applied science divi-sion of NOW, and the technology programme of the Ministry of Economic Affairs. F. Meersman is a post-doctoral fellow ofthe Research ... activity isthe most important parameter, but the loss of structure can be measured over a widerrange of temperature and pressure. Very often, enzymeinactivation that is due to a small change in the...
... emphasis of this report is in understanding the roles of bonus payand civilian airline hiring in pilot attrition; other factors are used in the modelsprimarily to help isolate the effects ofthese ... the accession data is information about the officer at the time of separation if the officer is no longerin the service, or information about the most recent year of service if the officer is still ... eligibilityvariable is likely to be mismeasured.Another variable included in the analysis isan indicator variable for reservecommission. This variable captures whether the original commission was reserveor...
... only one band wasamplified from each ofthe different samples andSouthern blot analysis using the probe prepared with the lac1 primers also revealed a single band. Further-more, all ofthese generated ... sequences of other basidiomycetelaccases (Fig. 7).Although the spectral characteristics ofthe lac1 proteinsuggest the absence of a type 1 copper site, this is not borneout by analysis ofthe deduced ... amplification ofthe clonedlac1 cDNA using the cycle number obtained from the left panel. Each value represents the mean ± SD of three PCR reactions.Fig. 4. RT-PCR analysis of transcription patterns of...
... that in the case of SGCI andSGCI[L30R, K31M] the total number of restraints is over500; this well exceeds the number of distance restraints usedfor the structure determination ofthe homologous ... ifference i n the internal ¯exibility of t he molecules. The rigid nature of the second b strand is consistent with the presence of the indole r ing of Trp25 bridging strands 1, 2 and 3. It should ... stabilize the binding loop. The hydrophobic core is organized differently in the twomolecules either b y a Phe ofthe đrst or by a Trp ofthe thirdb strand. The natural form of PMP-C is fucosylated...
... ATP-citrate lyase[24]. The results demonstrated the heteromeric structure o fthis enzyme and its role in regulating the d irection and flux of the RTCA cycle. For further understanding ofthe R TCAcycle, ... Bank of Japan(DDBJ). The sequence data was analyzed usingGENETYXsoftware package (Software Development, Tokyo, Japan). The nucleotide sequence data of Cl-idh will appear in the EMBL, GenBank, ... investigations of eachmember ofthe cycle. With respect to IDH, although the activities have been detected in some autotrophic organismsutilizing the RTCA cycle, no biochemical analysis of the enzyme...
... enzyme [2], and the chlorite dismutase is an iron protoheme IX-containing enzyme [3]. However,little is known about the mechanism of action of these enzymes. The name chlorite dismutase is unfortunate,because ... to the followingequation:Aobsẳ A0 BẵL=KdỵẵLịwhere B isthe maximum absorbance difference, [L] is the ligand concentration, Aobs is the observed absorbance, andA0 is the absorbance ... properties of GR-1 chlorite dismutase. Furthermore, we investigated the binding of hydroxide and imidazole to the ferric enzyme and of NO to the ferrous enzyme to establish the nature of the proximal...
... affects the electrostatic potential andquite often the conformation ofthe modified protein.Even in the absence of rearrangement, the changein the electric field and steric hindrance from aphosphate ... the mechanism of PHF for-mation, the effects of phosphorylation on the confor-mation and aggregation ofthe R4 repeat werestudied. Introduction ofthe phosphate ion, which predominantly carries ... comparison of the chemical shifts of NH and aH between the non-phosphorylated and phosphorylated peptides is sum-marized in Fig. 1. The chemical shift deviations of NHand aH reflect changes in the...