ttc0096 by nterminal amino acid sequence analysis and malditofms
Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt
... family.AbbreviationsATG, amino acid transporter glycoprotein; CSR, conserved sequence regions; GH, glycoside hydrolase; HAT, heteromeric amino acid transporter; hcHAT, heavy-chain subunits of heteromeric amino acid ... subunit ofthese amino acid transporters from the enzymesinvolved in the metabolism of starch and relatedsaccharides.Results and DiscussionEvolutionary relationships and sequence structural ... ourknowledge further.Materials and methodsAs a first step, all available sequences of hcHATs and hcHAT-like proteins were collected (Table 1) using the amino acid sequences of human 4F2hc (GenBankaccession...
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Tài liệu Báo cáo khoa học: Modulation of the enzymatic efficiency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx
... beingproline, aspartic acid, serine or alanine.Simple and double FNR mutants of amino acidsC266 and L268 were obtained and characterized. Itwas observed that alteration of the amino acid volumedecreases ... effect isprobably caused by amino acids C266 and L268, which face the other sideof this tyrosine. Simple and double FNR mutants of these amino acids wereobtained and characterized. It was ... the R amino acid groups introduced by mutations was calculated following the standard radii and volumes calculated by Tsai et al. [32], assuming a reducedstate of the cysteine. Amino acid hydropathy...
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Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants doc
... thatrequire tRNA for amino acid activation, the potentialfor misrecognition of related amino acids has beeninvestigated [9–13] and modulated by amino acid replacements and active site redesign ... tRNA and 0.35 nmol unlabelled tRNA was aminoacylated in a 10 lLtotal volume containing 50 mm Hepes pH 7.5, 10 mmMgCl2 and 2.5 mm ATP together with aminoacyl-tRNAsynthetase and amino acids ... on the fidelity with which aminoacyl-tRNAsynthetases (EC 6.1.1.x) recognize their cognate amino acid and tRNA substrates [1]. The mechanism(s) by which the family of aminoacyl-tRNA synthetasesmaintains...
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Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx
... synthesized by solid-phase methods on anABI 433A peptide synthesizer using standard Fmoc chemis-try and side-chain protection.N-terminal sequencing and MSN-terminal amino acid sequence analysis ... striking featureof conomarphin. The l -amino acid to d -amino acid epimerization in a polypeptide chain is quite rare and not well understood, although some d -amino acidshave been known for a long ... availableonline:Fig. S1. Natural and synthetic conomarphin with alll -amino acids on HPLC.Fig. S2. The fragments of natural conomarphin (A) and the synthetic l-Phe13-conomarphin (B) cleaved by trypsin on an...
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Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc
... contamination from xylem, and viceversa. Glx (glutamine and glutamate: five carbonamide and amino acid) and Asx (asparagine and aspartate: four carbon amide and amino acid) werefound to be the ... Light) and 24.6 ± 1.5 (xylem, Dark). The standard errors for individual amino acid contents are of the same order of magnitude as those of thetotal amino acid contents. Amino acid metabolism and ... Amino acid composition in leaves, and amino acid percentage ratio in the phloem exudates and leaves and xylem bleeding sap and leaves in the light and dark. The amino acid composition in leaves is...
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Tài liệu Báo cáo khóa học: Active-site residues and amino acid specificity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx
... before as important step in the biosynthesis of thelantibiotics epidermin and mersacidin catalyzed by theLanD enzymes EpiD and MrsD, respectively [8–10]. Flavin-dependent oxidative decarboxylation ... pQE12 and sequence analysis revealed a single point mutationwithin the AAVAD motif of CoaB proteins, namely Ala275 is exchanged for Thr275. (B) Dfp and Dfp A275T (¼ Dfp-1) were enriched by anionicexchange ... characterization of the dfp-1mutation, the dfp-1 gene was cloned into pQE12, sequenced and overexpressed. Sequence analysis revealed that dfp-1has a point mutation in codon 275 of the dfp gene,substituting...
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Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
... formed by amino acids 1–141. In this study, we used site-directed muta-genesis to generate point mutations and truncationsaround this position to explore the above prediction.As shown by Chen and ... trunca-tions and point mutations to C-terminal residues onSNase stability and conformation integrity was exam-ined by subjecting these mutants and wild-type pro-teins to CD [11,12] and differential ... unfolded by lowering its pH (for example, frompH 7 to pH 2). About 2.5 protons are associated withthe key glutamic amino acid residues at positions 75 and 129. This association between protons and...
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Tài liệu Báo cáo Y học: Importance of the amino-acid composition of the shutter region of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms pdf
... form, but could be reactivated by denaturation and refolding, either by SDS and refolding by dilution into abuffer with 1% BSA, or by guanidinium chloride and refolding by dialysis against NaCl/Pi. ... stressed and the relaxed confor-mations [15]. The network involves the side chains of the amino acids in positions 53 and 56 in a helix B, 186 in bstrand 3A, and position 334 in b strand 5A (Fig. ... a helices D and E forms a flexible joint, and b strands 3A and 5A slide apart in a shutter-like manner overthe underlying a helix B [14]. The central part of b strands3A and 5A and the N-terminal...
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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc
... and an increase incharged amino- acid residues (i.e. K, E, and R) inhyperthermophilic proteins. As S and T can catalyze thedeamination and backbone cleavage of Q and N residues[48,50], a reduction ... patterns in the amino- acid composition of hyperthermophilic proteins is the biasagainst thermally labile amino- acid residues. This pattern isobvious on examination of the amino- acid compositions ... the divalent metal ion in sugar binding, ring opening, and isomerization by D-xylose isomerase: replacement of a catalyticmetal by an amino acid. Biochemistry 33, 1488–1494.30. Hartley, B.S.,...
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Báo cáo khoa học: Amino acid limitation regulates the expression of genes involved in several specific biological processes through GCN2-dependent and GCN2-independent pathways ppt
... eight genesregulated by amino acid starvation using quantitativeRT-PCR. We selected genes that were either repressed(Hmgcs1) by amino acid starvation or induced by amino acid starvation in a ... mammals:(a) the target genes and biological processes regulated by amino acid avail-ability, and (b) the signaling pathways that mediate the amino acid response. Using large-scale analysis of gene expression, ... gene by the amino acid response and the endoplasmic reticulum stressresponse pathways occurs by common genomicelements. J Biol Chem 275, 26976–26985.Regulation of gene expression by amino acid...
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Báo cáo khoa học: Phytoene synthase genes in tomato (Solanum lycopersicum L.) – new data on the structures, the deduced amino acid sequences and the expression patterns doc
... and Psy2 genes and also provide the deduced complete amino acid sequences of the two enzymes, together with newinsights into the transcriptional regulation of Psy1 and Psy2 in chloroplast- and ... deduced amino acid sequences and the expression patternsGiovanni Giorio, Adriana Lucia Stigliani and Caterina D’AmbrosioMetapontum Agrobios, Metaponto, ItalyFruits are the mechanism by which ... almost identical and six introns being much more vari-able. For Psy1 and Psy2, respectively, the sequenced regions were 4527 and 3542 bp long, the coding sequences were 1239 bp and 1317 bp long,whereas...
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Báo cáo khoa học: Two L-amino acid oxidase isoenzymes from Russell’s viper (Daboia russelli russelli) venom with different mechanisms of inhibition by substrate analogs pdf
... lM ( ), 10 lM ( ) and 20 lM (·) OAB; and (F) NAT as indicated and 0 lM ( ), 5 lM ( ), 10 lM( ) and 20 lM (·) OAB.Inhibitor-binding sites ofL -amino acid oxidase S. Mandal and D. Bhattacharyya2088 ... indicated and 0 lM ( ), 1 lM ( ), 2 lM ( ), 5 lM (·), 10 lM (h) and 20 lM (D) OAB;(B) NATA as indicated and 0 lM ( ), 5 lM ( ), 25 lM ( ) and 35 lM (·) OAB; and (C) NAT as indicated and 0 lM ... followed at 450 nm. ReferenceFAD and eluted cofactor were collected and lyophilized forESI MS analysis. Enzyme assayl -Amino acid oxidase activity was followed by a coupledassay [22]. Hydrogen...
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Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
... [1,2,4,7]. Moreover, PAT1 and PAT2 mediated amino acid i nflux leads to a pronouncedintracellular a cidification [2,9] by cotransport o f t he zwit-terionic amino acid substrates and protons with a ... the amino group as in sarcosine. Interestingly, all testedprolines (L-Pro,D-Pro and L-OH-Pro) are recognized ashigh affinity substrates by PAT2 withD-Pro as the onlyD -amino acid, and L-OH-Pro ... spectrum thatincludes not only t he amino acids glycine, alanine, serine and proline but also osmolytes such as sarcosine and betaine, and theD-enantiomers of serine and alanine. Theapparent affinities...
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Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt
... and K9, and of G129 with K110 and K133. Other charged amino acids such as D73, D83 and E101 reinforce the interactionsof E75 and E129.H M. Chen et al. Local stability and key acidic amino acids ... Calculation of proteinextinction coefficients from amino acid sequence data.Anal Biochem 181, 319–326.Local stability and key acidic amino acids in staphylococcal nuclease H M. Chen et al.3974 ... Privalov and Potekhin [6].Local stability and key acidic amino acids in staphylococcal nuclease H M. Chen et al.3970 FEBS Journal 272 (2005) 3967–3974 ª 2005 FEBSnuclease studied by site-directed...
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Báo cáo khoa học: Comprehensive sequence analysis of horseshoe crab cuticular proteins and their involvement in transglutaminase-dependent cross-linking potx
... cDNA of acidic DE25 encoded a 137 -amino acid protein and a 22 -amino acid signal peptide(AB201776). A partial cDNA of acidic DE29 lackedthe 5¢-region and its N-terminal sequence determined by Edman ... Tyr(10–15%), Gly (16–19%), and Asp (10–12%). Proteinsin the basic QH group were abundant in Glu (13–16%) and His (7%) as determined by amino acid analysis, but their partial amino acid sequences indicated ... Asp (14%) and Glu (10–14%).N-Terminal amino acid sequence analysis ofHMM chitin-binding proteinsEleven of 37 spots observed on 2D SDS ⁄ PAGE wereresistant to sequence analysis by Edman degradation,presumably...
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- amino acid
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- the hypothalamic neuropeptides modulate physiological activity via g proteincoupled receptors gpcrs galaninlike peptide galp is a 60 amino acid neuropeptide that was originally isolated from porcine hypothalamus using a binding assay for galanin recep
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