the stably transfected human estrogen receptor α transcriptional activation assay for detection

Báo cáo khoa học: " Additive Estrogenic Activities of the Binary Mixtures of Four Estrogenic Chemicals in Recombinant Yeast Expressing Human Estrogen Receptor" pdf

Ngày tải lên : 07/08/2014, 15:20

... University Medical Center, USA) This yeast strain was used for the estrogenicity assay Growth of yeast for the estrogenicity assay The Saccharomyces cerevisiae ER + LYS 8127 cells were grown ... in the estrogenic activity However, the combinations of two estrogens at the high concentration produced lower estrogenic effects than that expected by summing the individual activities Fig Estrogenic ... Following two days culture, the yeasts were then allowed to grow until OD values at 600 nm reached between 1.0 and 2.0 Treatment of chemicals For the estrogenicity assay, the yeast cells were diluted...

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Báo cáo y học: "Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma" pdf

Ngày tải lên : 12/08/2014, 01:22

... find out the positivity rate between HPV detection and oral lesions We did not detect any type of low risk humanpapilloma virus in these lesions The HC-II assay used for the detection of the presence ... compare the efficacy of HC-II assay and PCR for the detection of specific HPV type (HPV 16 E6) in OSMF and OSCC cases as well as find out the prevalence rate of the HR-HPV in these lesions Materials ... 32.4% by the PCR and 31.5% by the HC-II test Therefore, the study concluded that slight difference was found between the positivity rate of HR HPV infection of the both detection methods in OSMF...

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Báo cáo khoa học: Sp3 transcription factor is crucial for transcriptional activation of the human NOX4 gene doc

Ngày tải lên : 29/03/2014, 00:20

... similarity between the rat and mouse sequences, the similarity of the human sequence was relatively low Therefore, we isolated the promoter region of the human NOX4 gene and examined its transcriptional ... essential for transcriptional activation of the human NOX4 promoter Figure shows the alignment of the 5¢-ﬂanking and 5¢-noncoding sequences of the human, rat and mouse NOX4 genes Compared with the high ... dramatically decreased by the deletion of GC-box1 The activity was further decreased by the deletion of GC-box2 (Fig 3B) As shown in Fig 3C, the transcriptional activity of the kb of the 5¢-ﬂanking region...

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Báo cáo y học: "The utility of pathway selective estrogen receptor ligands that inhibit nuclear factor-κB transcriptional activity in models of rheumatoid arthrits" pps

Ngày tải lên : 09/08/2014, 06:22

... interests The authors are employees of Wyeth Authors' contributions JCK, LMA and YL performed the in vivo experiments MF and LW performed the gene-profiling experiments LBM performed the cell-based assays ... (data not shown) The ERα was functional in these cells, because WAY169916 could effectively block the TNF -α- mediated inflammatory gene expression of IL-6, TNF -α, and ICAM-1 The potential involvement ... transcriptional inhibitors such as WAY-169916, the expectation is to accentuate the anti-inflammatory, anti-rheumatic activity observed with the non-selective estrogens Whereas nonselective estrogens...

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Dual activation of estrogen receptor a and aryl hydrocarbon receptor by the prenylflavone, icaritin restrict breast cancer cell growth and destabilize estrogen receptor a protein

Ngày tải lên : 04/10/2015, 17:05

... of human estrogen is estradiol Estrogens are produced and secreted by the theca and granulose cells of the ovaries The biological actions of estrogens are regulated by their concentration in the ... approaches include the use of anti-estrogens which binds competitively to the ERα and block the activation of the receptor The second approach is to target proteasomal degradation of ERα mediated primarily ... making estrogen or estrogen receptor targeted pathways for breast cancer therapy Estrogen receptor (ER) has been detected in more than half of the human breast cancer cases, with 70% of these...

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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

Ngày tải lên : 19/02/2014, 06:20

... characterization of the promoter region of the gene and the associated transcriptional factors Therefore, delineation of the transcriptional Promoters of human deoxyribonuclease I gene regulation of human ... numbers over the diagrams indicate the position of the corresponding nucleotides relative to the translation start site, and the numbers in parentheses below the diagram show the position of the corresponding ... represents the length of the product The scheme also shows the location of the reverse primer DN+89 used in another starting exon-speciﬁc PCR and quantitative real-time RT-PCR, the locations of the...

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Báo cáo khoa học: Dehydroepiandrosterone inhibits the proliferation and induces the death of HPV-positive and HPV-negative cervical cancer cells through an androgen- and estrogen-receptor independent mechanism pptx

Ngày tải lên : 16/03/2014, 00:20

... that DHEA binds to the androgen receptor and estrogen receptors a or b [38–41] DHEA at 30 nm is sufﬁcient to activate transcription of estrogen receptor b to the same degree as estrogen at its circulating ... related to the malignant state of the cells HeLa cells are an advanced-stage cervical cell carcinoma [27], in comparison with the other cell lines used for which no stage is speciﬁed; therefore, HeLa ... derived from human cervical cancers associated with human papilloma virus (HPV) and positive for the estrogen receptor, and to determine whether the effect of DHEA was dependent on its conversion...

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Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc

Ngày tải lên : 17/03/2014, 23:20

... In some experiments, the cells were washed and then grown in IL-2-free medium for 72 h, and were therefore considered as T cells deprived of IL-2 The subunits of the IL-2 receptor complex, class ... interaction with the IL-2Rb and c chains The observed difference between these and our results focuses attention on the constitutive or induced raftassociation of the IL-2R subunits and therefore the regulatory ... ice for 40 min, then incubated with anti-IgG (whole chain) RAMIG antibody at 37 °C, for 30 The cells were then ﬁxed with formaldehyde, blocked with isotype control antibody and stained with the...

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Báo cáo khoa học: An estrogen receptor a suppressor, microRNA-22, is downregulated in estrogen receptor a-positive human breast cancer cell lines and clinical samples pptx

Ngày tải lên : 22/03/2014, 21:20

... is the median; the upper and lower limits of the box are the 75th and 25th percentiles, respectively, and the vertical bars above and below the box indicate the maximum and minimum values The ... 0.0010 for the comparison between BT-474 and MDA-MB231, but P = 0.0399 for the comparison between MCF-7 and SK-BR-3, P = 0.0123 for the comparison between T-47D and SK-BR-3 and P = 0.0074 for the comparison ... alters the expression of microRNAs in human pancreatic cancer cells Mol Cancer Ther 7, 464–473 Pandey DP & Picard D (2009) MiR-22 inhibits estrogen signaling by directly targeting the estrogen receptor...

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Báo cáo khoa học: A proﬁle of the residues in the second extracellular loop that are critical for ligand recognition of human prostacyclin receptor pdf

Ngày tải lên : 23/03/2014, 07:20

... recognition pocket for the receptors, but the residues are less important for determining the receptor selectivities in binding to the different prostanoids This hypothesis is further supported by ... Prostacyclin receptor structure and function mapping of the residues that are important for receptor ligand recognition in the eLP2 region of human IP A proﬁle of the IP eLP2 residues involved in the receptor ... that the ligand recognition sites are probably in the same position for IP and TP Further mutations were made to characterize the properties of the side chains of the three key residues for IP For...

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Báo cáo khoa học: The Runx3 distal transcript encodes an additional transcriptional activation domain doc

Ngày tải lên : 23/03/2014, 09:20

... and the potential for transcriptional repression through HDAC5-mediated histone deacetylation, is similar for both isoforms (Fig 4C) Taken together, these results imply that the enhanced transcriptional ... contributes directly to transcriptional activation 3434 A novel activation domain in D-RUNX3 To analyze the transcriptional activation potential of the RUNX3 isoforms, independent of the runt domain ... survival and activation We were therefore interested to test whether there were differences in expression from the two promoters between the CD4+ and CD8+ T-cell subsets No primers suitable for real-time...

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Báo cáo khoa học: Insights into the activation of brain serine racemase by the multi-PDZ domain glutamate receptor interacting protein, divalent cations and ATP ppt

Ngày tải lên : 30/03/2014, 03:20

... modules For example, neither the PDZ4, nor the PDZ5 domain of GRIP bind to the GluR2 subunit of the AMPA channel independently, with the concerted action of both being necessary for the association ... the highest value The conformational change induce by GRIP binding on brain SR results in changes in both the Km and the Vmax for d-serine synthesis and the response curve for activation by calcium ... available on the Internet (see Experimental procedures) The results provided by the program were fully consistent in that all of them rendered the same hit with the highest score: the SR from the ﬁssion...

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Báo cáo khoa học: Identiﬁcation of the epitope of a monoclonal antibody that disrupts binding of human transferrin to the human transferrin receptor pptx

Ngày tải lên : 30/03/2014, 11:20

... epitope for the mAb F11 Based on these results, the epitope for the mAb F11 was localized to a region within residues 365–401 A nonspeciﬁc band at 66 kDa was observed in all the lanes for the mAb ... within the binding site of TF for the receptor but that binding of the antibody leads to steric hindrance or conformational changes that alter the binding site However, the agreement of the F11 ... in dimer formation The crystal structure for the recombinant form of the soluble TFR has been determined [9], and a higher resolution structure of the soluble TFR in a complex with the hemochromatosis...

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Báo cáo khoa học: Large-scale overproduction, functional puriﬁcation and ligand afﬁnities of the His-tagged human histamine H1 receptor ppt

Ngày tải lên : 30/03/2014, 14:20

... overhang The vector with the primer cassette was ligated with the EcoRI/XhoI fragment of the H1 receptor The resulting transfer vector pBacPAK9H1His containing the 10xHis-tagged human H1 receptor ... respectively The position of the intact Histagged H1 receptor is indicated by the arrow The quantity of the remaining minor contaminating bands in the puriﬁed receptor varied between preparations Their ... while the agonist histamine destabilizes the receptor Probably the inverse agonists reduce the dynamics and ﬂexibility of the protein, while an agonist has the opposite effect, making the receptor...

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Báo cáo khoa học: Internalization of the human CRF receptor 1 is independent of classical phosphorylation sites and of b-arrestin 1 recruitment potx

Ngày tải lên : 30/03/2014, 15:20

... demonstrated for several GPCRs [29] The function of b-arrestin is to uncouple the receptor from the G-protein, thereby mediating receptor desensitization, and furthermore to couple the receptor to the ... sauvagine could be observed in the presence of any of these inhibitors These ﬁndings indicate that neither the activation of PKA nor the activation of PKC is necessary for agonist-induced CRFR1 internalization ... following receptor activation (Fig 6) These ﬁndings indicate that b-arrestin is recruited to the receptors at the plasma membrane following receptor activation, but that Ó FEBS 2004 the CRFR1/b-arrestin...

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Báo cáo sinh học: "Histone modification enhances the effectiveness of IL-13 receptor targeted immunotoxin in murine models of human pancreatic cancer" doc

Ngày tải lên : 18/06/2014, 19:20

... together, our results provide support for testing this novel combination in the clinic for the therapy of human cancer including pancreatic cancer for which no therapeutic options are currently available ... anti -human IL-13Ra2 polyclonal antibodies (R&D, Minneapolis, MN) Tissue samples were fixed in 10% formalin solution for IHC and human cells were fixed by 4% paraformaldehyde (PFA) for ICC The ... transformed cells [25] Because of these characteristics, HDAC inhibitors are being tested in the clinic for cancer therapy Two HDAC inhibitors, SAHA and Romidepsin, are licensed by FDA for the...

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Báo cáo hóa học: " Higher polymerase activity of a human influenza virus enhances activation of the hemagglutinin-induced Raf/MEK/ERK signal cascade" pot

Ngày tải lên : 20/06/2014, 01:20

... of the HA protein triggers the activation of MAPK signaling [24] In this study, we therefore analyzed the expression of HA on the surface of MDCK cells infected with either virus (m.o.i = 1) The ... virus growth and infectivity [25-28] Therefore, we analyzed the potential role of these genes and the proteins they encode in more detail To investigate whether the H3N2 viral polymerase genes possess ... activity than those of the H1N1 subtype, we performed a luciferase assay using a minigenome system The pol I-driven plasmid encoding the luciferase gene was cotransfected into the human embryonic kidney...

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báo cáo hóa học:" Histone modification enhances the effectiveness of IL-13 receptor targeted immunotoxin in murine models of human pancreatic cancer" pot

Ngày tải lên : 20/06/2014, 03:20

... together, our results provide support for testing this novel combination in the clinic for the therapy of human cancer including pancreatic cancer for which no therapeutic options are currently available ... anti -human IL-13Ra2 polyclonal antibodies (R&D, Minneapolis, MN) Tissue samples were fixed in 10% formalin solution for IHC and human cells were fixed by 4% paraformaldehyde (PFA) for ICC The ... transformed cells [25] Because of these characteristics, HDAC inhibitors are being tested in the clinic for cancer therapy Two HDAC inhibitors, SAHA and Romidepsin, are licensed by FDA for the...

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Báo cáo y học: "The active metabolite of leflunomide, A77 1726, increases the production of IL-1 receptor antagonist in human synovial fibroblasts and articular chondrocytes" ppsx

Ngày tải lên : 09/08/2014, 01:23

... FCS, the cells were incubated with the various agents in low-serum (0.5% FCS) medium The cells were preincubated with A77 1726 or indomethacin for hours before stimulation with IL-1β or TNF -α for ... IL-1Ra production induced by IL-1β, or whether it could also be observed in the presence of other proinflammatory cytokines, such as TNF -α As in the case for IL-1β, A77 1726 markedly and dose-dependently ... in the presence of TNF -α (Fig 3) The effect of A77 1726 was highest at 100 µmol/l Furthermore, stimulation of IL-1Ra secretion by A77 1726 in human synovial fibroblasts was also dependent on the...

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Báo cáo y học: "Suppression of the inflammatory response in experimental arthritis is mediated via estrogen receptor α but not estrogen receptor " pot

Ngày tải lên : 12/08/2014, 14:21

... of estrogens, treatment in most of the animal models described so far involved the use of estrogen injections or implantation of estrogens such as E2 We used the synthetic estrogen EE, synthesized ... area under the curve ER, estrogen receptor that estrogen- mediated suppression in rat AA is ERα mediated The estrogen- mediated suppression of the TT-DTH in wild type mice is dependent on ERα Next, ... ERA-63, the ERβ-agonist ERB-79 or the non-selective estrogen EE whereby the ERα-sensitive uterus weight was used as a pharmacodynamic readout control For ERB-79, the more sensitive marker of epithelial...

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