... from cancer at any stage ofthe disease with the hope of raising awareness ofthe types of therapies that may be appropriate and increasing awareness ofthe role ofthe pain specialist in cancer ... towards the end of life, but also pain at diagnosis, as a consequence of cancer therapies and in cancer survivors. The document relates the science of pain to the clinical setting and explains the ... understanding of their condition.ã what the pain means to the individual and their family.ã how the pain may impact upon relationships within the patients family.ã whether the pain inuences the patients...
... from the same volume of whole serum with QIAmp Blood Midi Kit (QIAGEN, Valencia, CA) according to the manual. Bacterial DNA was also extracted fromthe bac-terial particles trapped fromthe ... spe-cific for 16S rRNA, another one – with the primers for ompA in nested PCR format. As can be seen from Figure 2, the sensitivity of PCR reaction was similar regardless ofthe primer set used. ... may originate from destructed mononuclear cells and/or macrophages residing in the atherosclerotic plaques. However regardless ofthe origin, we can claim that the presence of virulent chlamydial...
... analyses ofthe predictedamino acid sequence showed that the C-terminal half of the RepA ofthe pIT3 plasmid is sequence-similar to the helicases ofthe phage-encoded superfamily III pro-teins. The ... comparablewith the two-metal-ion mechanism of both RNA andDNA synthesis [17].In a previous study, we reported the findings of ananalysis ofthe complete sequence ofthe cryptic plas-mid pIT3 isolated from ... account for the promiscuous nature of the synthesis functions performed by the prim–poldomain and to discriminate between the functions of in vitro primase and polymerase.Another finding of our biochemical...
... cleft, the putative catalytic zinc being at the bottom ofthe cleftand a lobe containing the structural zinc at the mouth of the cleft and thus exposed to the solvent [8]. Grad-ual depletion ofthe ... Novagen.Expression of Pt-gdh under the control of araB promoterwas induced for 4 h at 30 °C by the addition of 0.2% ara-binose when the A600 of the growing culture reached 0.5. The cells from a 1-L ... tested, i.e. from 0.05 to 1 mm. The influence of Zn2+on the pH stability of GdhA is most evidentafter incubation (1 h, 55 °C) at pH 3.5, where, in the presence ofthe metal ion at 0.1 mm, there was...
... transfer from CDH to cytochrome c, Phe166, which is located in the cytochromedomain and approaches one of propionates of heme, was mutated to Tyr,and the thermodynamic and kinetic properties ofthe ... the special features of this enzyme. The optimum pH values of flavin reduction by cellobiose (pH 4.5–5.0) and of electron transfer from flavin to heme (pH 3.5–4.0) differ from each other, and the rate-limiting ... Thiswas expected fromthe thermodynamic result that the redox potential of heme in F166Y was lower than that of WT. However, when the electron transfer rate k(s)1) and the driving force DG...
... their positions. The arrowheads depict the residues important for the structural core ofthe IL-10 gene. The underlined amino acid residues are the signal sequences ofthe respective genes. The ... analysis investi-gated. This work will pave the way for further investigation of the biological function of this gene, and the probability of the presence of IL-10-related genes in fish, as seen inmammals.AcknowledgementsThis ... against products of b-actin. The x-axis indicates the time-periods of LPS incubation and the relative expression of the carp IL-10 gene on the y-axis. Controls for 0, 1, 3 and 6 h of incubation...
... 1).Subsite mappingOn the basis ofthe assumptions of Hiromi [30] that the intrinsic rate of hydrolysis (kint) in the productivecomplex is independent ofthe length ofthe substrate,Kmand ... Laboratory of Microbiology, Wageningen University, the Netherlands2 Laboratory of Food Chemistry, Wageningen University, the NetherlandsPectin is a complex polysaccharide present in the cellwall of ... (1973) Subsiteaffinities of glucoamylase: examination ofthe validity of the subsite theory. Biochim Biophys Acta 302, 362–375.L. Kluskens et al. An exopolygalacturonase from Thermotoga maritimaFEBS...
... (e.g., the height of a person), whilefor others it is only the center of a distribution(e.g., the weight of a coffee cup). Questionswith no trusted and exact answers were eliminated. From the ... information for the requested object, the requested object will receive the average ofthe extracted values ofthe whole set of the retrieved comparable objects and the com-parison step will be essentially ... value for the givenobject. During the first stage it is possible thatwe directly extract fromthe text a set of valuesfor the requested object. The bounds processingstep rejects some of these...
... years agoand supports the notion of a universal occurrence of hemocyanins in the Myriapoda [15,16]. At least threehemocyanin subunits were present at the time of divergence of the Chilopoda and ... the determination ofthe N-terminal sequences, C.Hunzinger for the MALDI-TOF analyses, C. Bache and J. Hermannsfor their help with the cloning experiments, and J. R. Harris forcorrecting the ... kDa, the isoelectricpoints (pI) range from 5 to 6.5 (Fig. 2). MALDI-TOFanalyses of tryptic peptides show that the spots representfour distinct subunit types, as deduced fromthe pattern of identical...
... becollected from each seed word, and then checkedwhether each ofthe target terms was included in the system output. We counted the number of tar-get terms in the following five cases. The right ... total; the average number of output terms per input is 12.2terms. We checked whether each ofthe 610 termsis a correct related term ofthe original seed term byhand. The result is shown in the ... term.3. There is a definition or explanation ofthe term.4. There are several technical terms that are re-lated to the term.We have implemented the checking program of the first two conditions in the...
... RejoicedBranch Rickey and the Or igins of the Breaking ofthe Color LineHe really leads a double life – one with his conscience and the other with the employer who pays him one ofthe top salaries in ... was good, for the owners both of the Negro team and the local team. The attendance for the annual game wasoften the highest ofthe year.✶Parallel with serious Negro ball were the black clown ... IntroductionJerry Malloy 1 The Birth ofthe Cuban Giants: The Origins of Black Professional BaseballLee Lowenfish 15 When All Heaven Rejoiced: BranchRickey and the Origins ofthe Breaking of the Color Line...
... on the same plot ofthe valuesfound for de novo synthesis in the presence of 7.5 or 2.5 mMMnCl2with various amounts of EDTA, or in the presence of MnCl2only [11], as a function ofthe ... where the authors have identified the reverse productsand shown that the CMP activation ofthe reverse of synthesis is by a different mechanism from base exchange[20]. Further analysis ofthe ... showing that the endogenous level of CDP-DAG is too low to allow detectable synthesis. The level of PtdIns in the membranes does not allow synthesis of detectable PtdIns by exchange ofthe polar...
... quality model to estimate the dispersion function, the accuracy ofthe estimated emissions will depend on the ability ofthe model to reproduce the dispersion ofthe pollutants. Until now, ... observed at this period ofthe day. From 2 pm to 6 pm, the wind direction is oblique to the street axis and the wind speed is higher than in the morning. At these hours ofthe day, tracer ... which, C is the concentration of a particular pollutant in the street (g/m3 or mg/m3); E is the emission ofthe pollutant from road traffic in the street; F is a function describing the dispersion,...
... determines the diagnosis of TB for the majority of adults, positive culture results often arelacking for children. In the majority of cases, the diagnosis of pediatric TB is established by the triad of ... and analysis of epi-demiologic and other dataã Development of policies and procedures and of a planfor controlling TB, on the basis ofthe assessment of the problemã Assurance of diagnostic, ... health, their skills and capacities, the places where they work, the way they are organized, the equipment and systems available to them, and the fiscalresources they command) represents the public...
... the finding that at pH 10 and 75 °C, the inactivation of b-glucosidase also does not involveany loss of secondary structure [1].Conclusions The stability ofthe b-glucosidase fromthe hyperther-mophile ... 113 ofthe large volume change associated with the forma-tion of a free charge [30]. Hence, a reduction in the number of electrostatic interactions would increase the pressure stability ofthe ... addi-tion, the band at 1.0 GPa in Fig. 5C does notresemble the broad, featureless band typical of anunfolded protein. Taken together, these observationssuggest that the unfolding at the level of the...