the caspase dredd activates relish and is required for resistance to infection by gram negative bacteria
Báo cáo y học: "The HIV-1 Rev/RRE system is required for HIV-1 5’ UTR cis elements to augment encapsidation of heterologous RNA into HIV-1 viral particle" ppsx
... comparable to those of standard HIV-1 vectors The unique capacity of the heterologous vector to remain episomal exposes it to manipulation for therapeutic gene delivery purposes Episomal vectors have ... monitored by western blot analysis for the absence of nucleolin protein, compared to total cellular proteins RNA was purified from the cytoplasmic supernatant using the PARIS protein and RNA isolation ... microtome (HM450, ThermoFisher) were taken through the extent of the striatum and stored in phosphate buffered glycerol at -20°C Immunohistochemistry and Confocal Microscopy Double labeling for the...
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Báo cáo y học: "Aerosolized colistin for the treatment of nosocomial pneumonia due to multidrug-resistant Gram-negative bacteria in patients without cystic fibrosis" ppt
... appropriate infection control guidelines by medical and nursing hospital staff and A baumannii, resistant to colistin; and an increase of infections due to Gram- positive and Gram- negative pathogens, ... belongs to the same group of antibiotics, and has similar antimicrobial spectrum, usage indications and toxicities as colistin) in the nebulized form for the management of pneumonia due to Gramnegative ... Proteus and Serratia spp., inherently resistant to colistin Consequently, there is an urgent need to restrict the use of colistin use in order to minimize these risks Conclusion Inhaled colistin...
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Báo cáo y học: "Aerosolized colistin for the treatment of nosocomial pneumonia due to multidrug-resistant Gram-negative bacteria in patients without cystic fibrosi" pdf
... yet to emerge, breakthrough infections with inherently resistant organisms such as Gram- positive organisms (including methicillinresistant Staphylococcus aureus) and colistin-resistant Gram- negative ... nebulized therapy with colistin, or preferably with colistin sulphamethate, might be the ‘therapy of no choice’, in particular where resistance to carbapenems (the therapy of choice) and other classes ... the use of this therapeutic modality In addition, dosing strategies and dosing recommendations for the intrapulmonary application of colistin are currently lacking Although resistance to colistin...
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Báo cáo khoa học: Caspase-8- and JNK-dependent AP-1 activation is required for Fas ligand-induced IL-8 production ppt
... necrosis factor-related apoptosis-induced ligand (TRAIL) receptors signal the activation of NF-jB and JNK through distinct pathways [46], although whether or not caspase- 8 is required for TRAIL-induced ... abrogated by pretreatment of cells with pan -caspase inhibitor (Z-VADfmk) and caspase- 8 inhibitor (Z-IETD-fmk) but not by Fig JNK activation is required for the FasL-induced AP-1 activation and IL-8 ... and pRL-TK, and cultured for 16 h The cells were then pretreated with the indicated inhibitors (20 lM) or dimethyl sulfoxide (DMSO) (0.1%) for h, and further stimulated with FasL for h The AP-1...
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Báo cáo khoa học: Knock-out of the chloroplast-encoded PSI-J subunit of photosystem I in Nicotiana tabacum PSI-J is required for efficient electron transfer and stable accumulation of photosystem I pot
... bars are covered by the marker To monitor the photosynthetic electron flow through PSI during steady-state photosynthesis in vivo, we estimated the redox state of P700 in the light by measuring oxidation ... excess of LHCI, and a less stable PSI is also observed To distinguish whether it is the stability or the assembly of the PSI complex that is affected needs further investigation The reduced content ... Thus, PSI-J is not required for binding or the function of the peripheral antenna, or at least the PSI that is formed is unaffected by the missing PSI-J The measurements of the functional antenna...
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Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf
... unpublished This study This study [44] This study [45] This study [28] [28] This study although by this time the levels of the protein are very low (Fig 2D) The fusion Protein A–Nop53p is functional, ... probes The relative positions of the probes on the 35S pre-rRNA are indicated in (B) Bands corresponding to the major intermediates and to the mature rRNAs are indicated on the right-hand side The ... exonucleolytically to generate the mature 5.8SS rRNA A fraction of the 27SA2 pre-rRNA is processed at the 5¢-end by a different mechanism and, following processing at the remaining sites, gives rise to the...
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Báo cáo khoa học: YidC is required for the assembly of the MscL homopentameric pore potx
... the earlier study [13], is located adjacent to the centre of the pore-forming TM1 It is therefore conceivable that even a slight perturbation of the conformation of MscL, for example due to the ... Based on the structure of the Mycobacterium tuberculosis MscL homologue, this position is expected to be exposed and relatively distant from the membrane, and should therefore be accessible to externally ... functioning is critical for assembly of the ATP synthase subunits rather than their individual insertion into the membrane [26] If neither YidC nor the Sec machinery is absolutely required for membrane...
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Báo cáo khoa học: The b domain is required for Vps4p oligomerization into a functionally active ATPase potx
... This study This study This study This study This study This study This study This study This study This study This study This study This study This study This study This study This study This ... to Vps4p The amount of wild-type Vps4p-His6 or Vps4p-GAI-His6 bound to GST-Vps20 was quantified by densitometry The shift in the relative positions of the wild-type Vps4p-His6 and Vps4p-GAI-His6 ... destined for the cell surface into a pathway that takes it via endosomes to the vacuole In vps4D cells, CPY is missorted at the late Golgi into vesicles destined for the cell surface and secreted...
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Báo cáo khoa học: Hydrogen bond residue positioning in the 599–611 loop of thimet oligopeptidase is required for substrate selection pdf
... that of His231 and Tyr157 in thermolysin [21,22] His231 (analogous to Tyr612 in TOP) and Tyr157 (analogous to Tyr605 in TOP) work together in the FEBS Journal 275 (2008) 5607–5617 ª 2008 The Authors ... model of the closed form of TOP was generated by clipping TOP at the division between domains I and II (residues Leu156, Val351, Gln544 and Glu616), and separately fitting domains I and II to the structure ... Thus, a model of the closed form of TOP was created by separately fitting domains I and II of the open TOP crystal structure onto the structure of DcP in its closed form [19] The TOP domains superimposed...
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Báo cáo khoa học: The Drosophila jumonji gene encodes a JmjC-containing nuclear protein that is required for metamorphosis pot
... chromosomal distribution of mJmj and its ability to rescue the phenotypes of the djmj mutants To this end, transgenic flies that B E C A F D Fig The djmj gene is required for metamorphosis (A) Lethal ... of whether dJmj links with these proteins to control metamorphosis is clearly warranted The possible interaction domain of dJmj for these factors is the N-terminal repression domain, which is evolutionarily ... is widely expressed and is required for the correct development of various tissues, involvement in the regulation of a diverse range of developmental programs, not limited to cardiac cells, is...
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Báo cáo khoa học: The calcium-binding domain of the stress protein SEP53 is required for survival in response to deoxycholic acid-mediated injury pdf
... pro-oxidants, and refluxed acid and bile adducts These cells have therefore presumably evolved specific mechanisms to tolerate and repair injury induced by exposure to these and other damaging ... were required to give rise to significant apoptosis These latter levels were in the range we used (Figs and 3) and given this, we titrated DCA from low lm to > mm to determine A I B C whether ... CDCA and LA did induce apoptosis in the OE33 cell line, with the percentage of sub-G1 cells increasing to 25 and 23%, respectively (Fig 4E,F) Furthermore, the levels of apoptosis induced by these...
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Báo cáo khoa học: The predominant protein arginine methyltransferase PRMT1 is critical for zebrafish convergence and extension during gastrulation pdf
... layers and the body plan are established by directed and coordinated cell movements, including epiboly to cover the yolk cells by spreading the blastomeres, involution to internalize the marginal ... cells to form the precursors of the mesoderm and endoderm, and C ⁄ E, in which cells accumulate on the dorsal side and lead to axis formation Gastrulation begins at 50% of epiboly (6 hpf) and ends ... Methylation of R3 of histone H4 by PRMT1 is part of the epigenetic histone code critical for chromatin structure and transcriptional activation [19] Increased H4R3 methylation by the recruitment of...
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Báo cáo khoa học: DYRK1A phosphorylates caspase 9 at an inhibitory site and is potently inhibited in human cells by harmine pptx
... not due to caspase (Fig S1) This study shows that caspase is partially localized to the nucleus and its phosphorylation by DYRK1A is promoted by nuclear targeting and diminished by cytoplasmic ... caspase 7, leading to the organized disassembly of the cell [11] Regulation of apoptosome formation is controlled at the level of cytochrome c release from mitochondria by pro- and anti-apoptotic ... kinase activity towards caspase was sensitive to harmine and thus due to DYRK1A (Fig S2) Together, these results demonstrate that DYRK1A and caspase co-localize to the nucleus and that DYRK1A...
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Báo cáo khoa học: Activation of p21-activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells potx
... LPA [56] On the other hand, it has been shown that the PI3Kb isoform was important for the mitogenic activity of LPA in nonhematopoietic cells that not express PI3Kc [57] PI3Kb isoform is classically ... platelet-derived-growth-factor receptor, not the epidermalgrowth-factor receptor, is used by lysophosphatidic acid to activate p42/44 mitogen-activated protein kinase and to induce prostaglandin G/H synthase-2 ... with each of the dominant negative forms (N17Rac1, N17Cdc42 and N19RhoA) and the corresponding control vectors, and the cells were incubated in the absence or presence of lM LPA for PAK1 activity...
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Báo cáo Y học: Barley a-amylase Met53 situated at the high-affinity subsite )2 belongs to a substrate binding motif in the bfia loop 2 of the catalytic (b/a)8-barrel and is critical for activity and substrate specificity pot
... structure of the AMY2 (top) and TAA (bottom) complexes [16,17] The inhibitors are in green and the arrow indicates the nitrogen (in dark blue) that corresponds to the oxygen of the scissile glycosidic ... and therefore discarded Mass spectrometry conrmed the two forms were distiguished by Cys95AMY1 glutathionylation in the lower pI form [38,5052] The aromatic replacements Met53Trp/Tyr had no visible ... assistance, L H Sứrensen and the late B Corneliussen for amino acid analysis, and M.-B Rask and the late J Sage for DNA sequencing This work was supported by the EU 4th Framework Programme on Biotechnology...
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Báo cáo sinh học: " A conditional-lethal vaccinia virus mutant demonstrates that the I7L gene product is required for virion morphogenesis" pptx
... Traktman for the vTetR virus strain and for helpful discussions, Tove' Bolken and Dr Marika Hedengren-Olcott for helpful discussions, and Dr Michael Nesson for the electron microscopy analysis This ... there is still some processing of p4b in the absence of TET and yet the morphology of the mutant virus in the absence of TET shows only immature virus particles suggests the hypothesis that there ... dependent on the presence of TET, while the wild-type virus is unaffected by either the presence or absence of TET To determine the optimum TET concentration required for replication of vtetOI7L, TREx-293...
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Báo cáo hóa học: " The YPLGVG sequence of the Nipah virus matrix protein is required for budding" pdf
... (NiV) and Hendra virus (HeV) are emerging members of the family Paramyxoviridae that are distinguished by their ability to cause fatal disease in both animal and human hosts, and comprise the genus ... NiV M (unpublished observation) To determine whether the conserved YPLGVG sequence is required for HeV M release, we constructed HeV M P93A and Δ92–97 mutants and tested them in the budding assay ... shown), possibly due to mis-folding and degradation These data suggest that the YPLGVG sequence is required for NiV and HeV M budding, and may also play a role in HeV M stability The YPLGVG matrix...
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báo cáo hóa học:" A conditional-lethal vaccinia virus mutant demonstrates that the I7L gene product is required for virion morphogenesis" potx
... Traktman for the vTetR virus strain and for helpful discussions, Tove' Bolken and Dr Marika Hedengren-Olcott for helpful discussions, and Dr Michael Nesson for the electron microscopy analysis This ... there is still some processing of p4b in the absence of TET and yet the morphology of the mutant virus in the absence of TET shows only immature virus particles suggests the hypothesis that there ... dependent on the presence of TET, while the wild-type virus is unaffected by either the presence or absence of TET To determine the optimum TET concentration required for replication of vtetOI7L, TREx-293...
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Báo cáo y học: "Pro-apoptotic Bid is required for the resolution of the effector phase of inflammatory arthritis" pdf
... prepared the arthritis model, and performed the ELISAs and apoptosis assays JH performed all flow cytometry work EB assisted with all measurements for arthritis GKH was the pathologist who analyzed the ... which is cleaved by caspase following death receptor ligation Cleaved Bid is targeted to the mitochondria and ultimately results in the induction of apoptosis mediated by the mitochondrial apoptotic ... space, where it binds to Apaf1, forming the apoptosome This complex leads to the activation of initiator pro -caspase [18,19] Caspase activates caspases and [20], which then induce the downstream degradative...
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báo cáo khoa học: "The grapevine guard cell-related VvMYB60 transcription factor is involved in the regulation of stomatal activity and is differentially expressed in response to ABA and osmotic stress" ppt
... and 96) On the other hand, the grape accession protein ABK59040 was closely related to AtMYB30 and AtMYB31 and to a lesser extent to AtMYB94 and AtMYB96 (Figure 1A) Hereafter, we will refer to ... investigate the role of VvMYB60 in the regulation of stomatal activity and to Page of 14 explore the conservation of the MYB60 gene function between grape and Arabidopsis To this end, we introduced the ... expression was higher before veraison, when the grape berry is photosynthetically active and stomata are functional, and was reduced after veraison, when stomata evolve into non-functional lenticels...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25