studies on growth crystal structure and characterization of novel organic nicotinium trifluoroa
Báo cáo Y học: Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod pdf
Ngày tải lên :
08/03/2014, 23:20
... O-Glycan fractions were subjected to permethylation [13] prior to MALDI-TOF MS analysis Removal of O-acetyl groups Alkylation, enzymatic digestion and peptide separation For in-gel digestion, the proteins ... N-Terminal sequencing of the kininogens was only successful from the 42-kDa band of wolffish kininogen (XLVQPGVLI…, Table 1) The major bands of both kininogens and also the 60-kDa band of cod kininogen ... salmon kininogen [6], and it is possible that the poor staining may be partly the result of extensive glycosylation Both bands of the wolffish inhibitor were investigated by in-gel digestion and...
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Báo cáo khoa học: Identification and characterization of novel PKA holoenzymes in human T lymphocytes pdf
Ngày tải lên :
16/03/2014, 18:20
... recognized the 47 kDa band in T-cells, and the 40- and 47 kDa protein bands in Jurkat cells (C) Linear depiction of Ca1, Cb1 and Cb2 amino acid sequence and the localization of the domains ⁄ antigens ... p50csk [16] and Raf-1 [17] in the early phase of the stimulatory process and regulation of interleukin-2 production through phosphorylation of the nuclear factor of activated T cells [18] and nuclear ... labelling demonstrated > 90% CD3 positive cells by flow cytometry Construction of expression vectors and culture and transfection of 293T cells Human cDNAs encoding full-length Ca1 and Cb2 were...
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Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx
Ngày tải lên :
18/03/2014, 01:20
... with a Mr of 26 kDa on SDS/PAGE under nonreducing conditions and 29.7 kDa under reducing conditions (Fig 2D, inset) From this purification, we obtained mg of purified ablomin from g of crude venom ... fractionated by gel filtration on a Superdex 200 pg column The pooled fraction (bar) contained purified tigrin (inset, SDS/PAGE; R, reducing conditions; NR, nonreducing conditions) (C) Primary structure ... identical to the corresponding residues in HLTX (Fig 4) The effects of tigrin and ablomin on rat tail arterial contraction Fig Isolation of tigrin from the Duvernoy’s glands of R tigrinus tigrinus...
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facile synthesis and characterization of novel nanocomposites
Ngày tải lên :
19/03/2014, 16:48
... because of the existence of a bending vibration of H O H at 1630 cm−1 , and a strong stretching vibration of O H Fig EDX spectrum of the nanocomposites prepared by hydrothermal reaction at 140 ... delicate composite structure was achieved through a simple hydrothermal reaction of common rutile TO2 powders and a concentrated NaOH solution Fig 1d is a low magnification TEM image of the products ... carbon at 284.8 eV Transmission electron microscopy (TEM) and high-resolution transmission electron microscopy (HRTEM) images, which were obtained using a JEOL TEM-2010F at acceleration voltage of...
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synthesis and characterization of novel ferromagnetic ppy-based nanocomposite
Ngày tải lên :
20/03/2014, 13:08
... deformation vibration of NH+ which is formed on the PPy chains by protonation at 1101 cm− [16], C–H out -of- plane ring deformation vibration at 795 cm− and the C–C out -of- plane ring deformation vibration ... magnetic field of 10 kOe Results and discussions The structures of the PPy/Zn0.5Cu0.5Fe2O4 nanocomposite were investigated by FTIR spectroscopy and X-ray diffraction FTIR spectra of the PPy and PPy/Zn0.5Cu0.5Fe2O4 ... behavior and a similar value of saturation magnetization, however, the coercivity, which is related to the microstructure, is larger than that of the sample obtained by w/o microemulsion route...
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Báo cáo khoa học: Purification and characterization of novel salt-active acharan sulfate lyase from Bacteroides stercoris HJ-15 ppt
Ngày tải lên :
23/03/2014, 21:20
... under the standard reaction conditions These lyase activities on other sulfated polysaccharides were also measured One milligram of each substrate was added to the reaction mixture Because of their ... Purification and characterization of novel chondroitin ABC and AC lyases from Bacteroides stercoris HJ-15, a human intestinal anaerobic bacterium Eur J Biochem 269, 2934–2940 16 Kim, B.T., Hong, S.H., ... [16] Table Effect of divalent metal ions on salt-active acharan sulfate lyase activity Metal iona Control Mg2+ Cu2+ Ni2+ Co2+ Mn2+ Ca2+ Zn2+ Pb2+ EDTA Fig Effect of pH on the activity of salt-active...
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Báo cáo khoa học: Identification and characterization of novel salivary thrombin inhibitors from the ixodidae tick, Haemaphysalis longicornis doc
Ngày tải lên :
31/03/2014, 01:20
... and the mixtures added to prewarmed substrate solution The prolongation of fibrin clot formation was measured using the coagulometer Assay of effects of madanin and on activation of factor V and ... Platelet aggregation was monitored using an aggregometer Results cDNA cloning and expression of madanin and Three distinct cDNA libraries were constructed from the salivary glands of H longicornis at ... association constant, and C is the concentration of the analyte used in the assay Kd was derived from the relationship, Kd ¼ 1/Ka Under the conditions of this experiment, Kd values for binding of madanin...
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Báo cáo Y học: Purification and characterization of novel chondroitin ABC and AC lyases from Bacteroides stercoris HJ-15, a human intestinal anaerobic bacterium pptx
Ngày tải lên :
31/03/2014, 23:20
... markers Characterization of two chondroitin lyases When the molecular masses of chondroitin ABC and AC lyases under nondenaturing conditions were determined by gel filtration, chondroitin ABC and ... activity for chondroitin sulfate C and hyaluronic acid was Table Effect of divalent metal ions on the activity of chondroitin lyases Final concentration of divalent ion, mM 0.03 U of the homogenously ... the previously purified chondroitin lyases In conclusion, this is the first report on the purification and characterization of chondroitin ABC and AC lyases, particulary chondroitin AC lyase, from...
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Báo cáo hóa học: " Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus" pot
Ngày tải lên :
20/06/2014, 23:20
... Inhibition of P excavatus proteases Figure Profiling of P excavatus protease fractions: (A) Size exclusion chromatogram of FIII-3 and FIV presented their fractionation into two peaks, ones of which ... FIII-3a and FIII-3b, with a MW of 33 and 31 kDa, respectively, and only the latter one showed protease activity Both fractions had a band of 33 kDa on SDS-PAGE gel Besides, FIII-3b contained ... purification and characterization of xylanasefrom Staphylococcus sp SG-13 and its application in biobleaching of kraft pulp J Appl Microbiol 88(2):325–34 Gupta GS, Singh GP (1983) Isolation and characterization...
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synthesis and characterization of novel polymers for functional and stimuli responsive silicon surfaces
Ngày tải lên :
13/11/2014, 11:09
... on a covalent modification approach and on the use of non-covalent but specific associations The first part will include a discussion on the modification, characterization, and applications of ... investigation of the influence of surface adenine concentration on polymer recognition of adenine-modified surfaces The influence of multiple hydrogen bonding groups on the association of adenine-containing ... spectra of a) & c) N1s region, and b) & d) P2p region on modified silicon/SiO2 surfaces 226 Figure 6-4: Influence of surface adenine concentration on the extent of PS-thymine recognition by silicon/SiO2...
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Identification and characterization of novel anticoagulants from bungarus fasciatus venom
Ngày tải lên :
09/09/2015, 11:08
... Discussion and Conclusion Chapter Identification and characterization of novel inhibitors on extrinsic tenase complex from Bungarus fasciatus (banded krait) Venom 3.1 Introduction 3.2 Materials and ... extension of second loop in long-chain 3FTx due to fifth disulphide bridge is shown in red color Figures and legend cited from “Kini, R.M and R Doley, Structure, function and evolution of three-finger ... 4.3.8 Inhibition kinetics of rFasxiatorN17R,L19E 127 4.3.9 rFasxiatorN17R,L19E prolongs FeCl3-induced carotid artery thrombosis 130 4.4 Conclusion and Discussion 133 Chapter Conclusion and Future...
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Identification and characterization of novel proteins from a rare australian elapid snake drysdalia coronoides
Ngày tải lên :
11/09/2015, 10:02
... Introduction 41 Materials and Methods 42 iii Reagents and kits; Collection of venom and venom gland; RNA isolation and cDNA synthesis; Cloning of ds cDNA; Isolation of plasmids and verification of clones; ... purification; Cloning of synthetic gene into pET-32a and pET-M; Transformation of cloning and expression host strains; Expression of protein; Purification of protein; Mass determination; Refolding of ... Proteomics of crude venom of Drysdalia coronoides Description of novel proteins Chapter 69 75 Cloning, Expression and Purification of Recombinant Drysdalin Introduction 79 Overview of pET system;...
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Cloning, expression and characterization of novel helicobacter pylori differentiating antigen heat shock protein 20
Ngày tải lên :
12/09/2015, 10:19
... information for studying the evolution of H pylori and epidemiology of H pylori infection 2.3.4 Evolutionary change of DNA sequences 2.3.4.1 Synonymous and non-synonymous substitutions Synonymous ... residues of HSP20 RESULTS 4.1 Preparation of recombinant HSP20 (rHSP20) 85 4.1.1 Construction of rHSP20 expression vector 85 4.1.2 Expression and purification rHSP20 protein 87 4.2 Preparation and characterization ... encouragement and understanding i Table of contents Table of contents Content Page ACKNOWLEDGEMENT i LIST OF FIGURES ii LIST OF TABLES v LIST OF ABBREVIATIONS vii LIST OF PUBLICATIONS xi SUMMARY...
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Identification and characterization of novel group 5 and group 21 allergens from dust mite and ige binding epitope mapping of blo t 5
Ngày tải lên :
14/09/2015, 12:44
... Blo t 21 and 77 Blo t 3.2.11 Localization of Blo t 21 and Blo t on sections of mite 79 body 3.2.12 3.3 Concentration of Blo t 21 in the house dusts 81 Discussion Chapter 4: 84 Effect of Temperature, ... organization of the gene encoding Der f 21 and 111 Der f 5.2.3 Southern blot of Der f 21 and Der f 116 5.2.4 Secondary structures of Der f 21 and Der f 118 5.2.5 IgE-binding of Der f 21 and Der f in consecutive ... f 21 and Der f Der f 21 and Der f in house dust samples 130 Discussion 132 135 Chapter 6: Identification and Characterization of Group and Group 21 in S medanensis and Cross Comparison of a Panel...
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Synthesis and characterization of novel jacketed polymers and investigation of their self assembly and application 6
Ngày tải lên :
16/09/2015, 17:11
... precise placement and functional control of shape-persistent polymers Stiff polymers, combined with precise molecular placement, are good candidates for the construction of fine microstructure Compared ... transition at 105 °C X-ray diffraction experiments indicate a rectangular column-type mesophase with the lattice constants a = 35.87 Å and c = 58.92 Å Studies on structureproperty relationship of ... incorporation of the polar functional groups A series of novel jacketed polymers were synthesized according to this strategy Strong electrostatic, weak van der Waals interactions, and geometrial...
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Synthesis and characterization of novel jacketed polymers and investigation of their self assembly and application 5
Ngày tải lên :
16/09/2015, 17:11
... The effect of the concentration of chromophoric polymers on sensitivity of the photo resists The good solubility and lack of crystallization of the chromophore-immobilized polymers 3a and 3b in ... properties of polymers 3a, 3b and 3c were measured in chloroform solution The absorption and emission spectra of the polymers are shown in Fig.5.3 and the values as given in Table 5.2 The UV spectrum of ... anthracene groups onto a polymer backbone and used the polymer as potential photoimageble materials The arrangement and the high concentration of the chromophores on the polymer backbone gave good...
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Synthesis and characterization of novel jacketed polymers and investigation of their self assembly and application 4
Ngày tải lên :
16/09/2015, 17:11
... shifts of the absorption bands of functional groups provide information on the nature and intensity of the interactions Figure 4.1 shows the FTIR spectra of P1(DBSA)x, where the x is 0.5, 1.0 and ... increase of the peak at around 1190 cm -1 means the increase of the SO3- groups upon deprontonation, which support the formation of the complexes A shift of 57 cm-1 corresponding to the formation of ... side-chain, and network structures, have been prepared by the self-organization of polymers and small molecules The noncovalent interactions play a key role in the formation of assembled structures, and...
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Synthesis and characterization of novel jacketed polymers and investigation of their self assembly and application 3
Ngày tải lên :
16/09/2015, 17:11
... degree of polymerization (DP), the conical monodendrons assemble to produce a spherical polymer with a random-coil conformation for the polymer backbone With the increase of DPs, the monodendritic ... representation of lamellar structures of the novel jacketed polymer, d1 is the Smectic layer distance, and d2 is the diameter of the cylindrical core, which comprised of mesogenic units and the backbone ... Results and Discussion 3.3.1 Synthesis of polymers The polymers were synthesized through radical polymerization from the appropriate monomers The concentration of initiator BPO was 1.0 mol % based on...
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Synthesis and characterization of novel jacketed polymers and investigation of their self assembly and application 2
Ngày tải lên :
16/09/2015, 17:11
... Schematic representation of rectangular columnar structures of the novel jacketed polymer 2.2.3.4 Influence of the pyrimidine functional groups and the alkyl chains on the self-assembly of the jacketed ... cylindrical structure with longrange order inside a rectangular columnar structure The study on the structure- property relationship of the polymers based on the pyrimidine functional groups and the ... self-assembly of polymer P01, P02, P03 and P04 based on the results from DSC, POM, X-ray diffraction and AFM analysis The bulky polymer P04 only shows a glass transition in DSC and X-ray diffraction results...
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Synthesis and characterization of novel jacketed polymers and investigation of their self assembly and application
Ngày tải lên :
16/09/2015, 17:11
... 13-15 They demonstrated a rational control of polymer conformation through self-assembly of monodendritic side - groups At low degree of polymerization (DP), the conical monodendrons assembled ... spatial arrangement of a polymer chain This stiffness may contribute to the formation of supramolecular structures and is used to demonstrate the correlation between chemical structure and performance ... The order of macromolecules depends largely on the primary structure and the segmental interaction of polymers It is well known that the chain stiffness of polymers offers a method of controlling...
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