structure function analysis of y family pols iv and v of e coli
Báo cáo khoa học: Structure ⁄function analysis of spinalin, a spine protein of Hydra nematocysts doc
... desmoneme are placed inside the middle of the spiral-like tubule so that the bristles of a prey are firmly fixed Immunocytochemistry revealed strong spinalin staining in the center of the everted ... peptide (B) Overview of a discharged desmoneme visualized by immunofluorescence (B¢) and phase contrast microscopy (B) Scale bar ¼ 20 lm (C) Close view of the expelled tubule and spines of a desmoneme ... kDa) However, analysis by ESIMS revealed a mass of 23706.0 Da for the reduced protein (calculated mass of the reduced protein, 23 700.8 Da) The agreement between measured and calculated molecular...
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Báo cáo khoa học: Structure–function analysis of the filamentous actin binding domain of the neuronal scaffolding protein spinophilin pot
... ubiquitously expressed, is predominantly found in neurones, whereas neurabin is expressed almost exclusively in neuronal cells, generally at lower levels than spinophilin Despite their similarity, they ... Because of their extensive overlap, we did not attempt to measure the length of crosslinked actin polymers NMR spectroscopy Acknowledgements NMR measurements were performed at 298 K on a Bruker AvanceII ... may indicate that the region 154–305 modulates the relative angle of the two putative actin binding sites On the basis of published data, this may also be caused by different effective concentrations...
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STRUCTURE-FUNCTION ANALYSIS OF CXXC FINGER PROTEIN 1
... foundation of hard work and dedication in me My parents have provided me with everything I have ever needed to be where I am today and have always been there for me Also, I want to thank my parents ... 5-methylcytosine base could be removed by a glycosylase or through nucleotide excision and then be replaced by an unmethylated cytosine Many groups have reported 5-methylcytosine-DNA glycosylase ... patterns during early embryonic development (Yokochi 2002) Dnmt3a and Dnmt3b enzymes are highly expressed during early developmental stages and prefer unmethylated DNA more than hemimethylated...
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Báo cáo y học: "What does the structure-function relationship of the HIV-1 Tat protein teach us about developing an AIDS vaccine?" ppsx
... Figure Tat sequences representative of the five main HIV-1 subtypes Tat sequences representative of the five main HIV-1 subtypes The sequence length of Tat is variable and ranges from 86 to 101 residues ... while the functional region II, IV and V are well exposed to the solvent The extra residues in the C-terminus of Tat Eli are exposed to the solvent and protrude from a groove between the basic region ... new born children from HIV-1 infected mother succeed to escape HIV-1 infection that can occur during the delivery or the breastfeeding and generally sero-revert when they are eighteen months old...
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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt
... domain enzymes with a tyrosine at the switch position have a relatively smaller active site volume and are predominantly monomethyltransferases Although site-directed mutagenesis studies have validated ... methyltransferases based on the presence of a conserved tyrosine at the Phe ⁄ Tyr switch position However, mono-, di- and trimethylation activities have been attributed to SET1 family complexes ... where the structures have been determined [42], the overall structure of the MLL1 SET domain contains two canonical conserved regions, SET-N and SET-C, that are separated by a less conserved...
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Báo cáo khoa học: New insights into structure–function relationships of oxalyl CoA decarboxylase fromEscherichia coli pptx
... oxalate and even use it as an exclusive energy source [1] Oxalyl CoA represents an activated form of oxalate and is decarboxylated by the thiamine diphosphate (ThDP)-dependent enzyme oxalyl CoA decarboxylase ... density of the side chain of Y4 78 is well defined The same is true for the corresponding residue Y4 83 in the crystal structure of OfODC No significant differences were found between the overall structures ... Studies on oxalyl CoA decarboxylase of E coli T Werther et al A Fig Stereo view of the crystal structure of EcODC (A) Schematic representation of the EcODC monomer Yellow arrows indicate b sheets,...
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Báo cáo khoa học: Studies on structure–function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway ppt
... signals) and to the b-CH2 of the pyruvyl part (singlet at 4.15 p.p.m., relative integral of 2), respectively As the ketone of indolepyruvate seems to be the true substrate species of EcIPDC catalysis, ... substrates studied in this work (pyruvate, the native substrate of PDC, benzoylformate, the native substrate of benzoylformate decarboxylase together with the 4-substituted derivatives, indolepyruvate, ... indolepyruvate EcIPDC is unable to decarboxylate freshly prepared solutions of indolepyruvate Therefore, the chemical properties and purity of indolepyruvate were characterized by H NMR spectroscopy...
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Báo cáo khoa học: Structure–function relationship of novel X4 HIV-1 entry inhibitors – L- and D-arginine peptide-aminoglycoside conjugates pptx
... dioxane (1 : : 1, v ⁄ v ⁄ v) medium in the presence of two equivalents of triethyl amine for h at room temperature After removal of the solvents under reduced pressure, the FITC derivatives were ... to cells FITC derivatives of d-arginine (6- and 9-mers) and their aminoglycoside-neamine and -neomycin conjugate FITC derivatives (for FITC derivatives preparation, see Experimental procedures) ... described in Experimental procedures Cytotoxicity was measured by trypan blue exclusion assay for MT2 cells The data are the average of three independent experiments The antiviral experiments were...
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Báo cáo khoa học: Effect of valine 106 on structure–function relation of cytosolic human thymidine kinase Kinetic properties and oligomerization pattern of nine substitution mutants of V106 ppt
... ATP and 2.5 mM MgCl2 were included in the dilution buffer Enzyme kinetics The kinetic parameters and the degree of co-operativity were determined as previously described [18] The experimental ... parameters are given in Table The substrate kinetics of the dimeric enzymes V1 06A, V1 06I and V1 06T (Fig 4A) is essentially the same as that previously observed for V1 06WT [18] and for the endogenous ... dimer–tetramer transition, as well Taken together, these observations imply that the shift in TK1 between a low activity dimer with apparently negative co-operativity and a high activity tetramer...
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Structure function relationships of variegin a novel class of thrombin inhibitors
... also reveals other important information and facilitates subsequent design of variegin variants These variants that have been designed and characterized cover a diverse spectrum of potency, kinetics ... peptide have an overall positive charge which might help to target the molecule to the negatively charged surface on exosite-I (van de et al., 1995) The roles of the additional domains revealed by ... pathway In the collagen pathway, the exposure of subendothelial collagen recruits platelets to the site of injury Platelets adhere to the exposed collagen through plateletglycoprotein VI and platelet...
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Structure function studies of vesicle associated membrane protein associated protein b (VAPB) associated with amyotrophic lateral sclerosis (ALS
... expression of the alternative splice variants of the VAPB gene has never been demonstrated in mammalian tissues or organs They were, however, detected by Nachreiner et al., (2010) only at the mRNA level ... of pool lysates of various organs prepared from several people that several bands detected by Anti-VAPC antibody despite being smaller than the expected size of VAPC were observed in the stomach, ... the mutant protein, they too become stressed and eventually undergo cell death Collectively, these observations support the hypothesis that VAPB (P56S) specifically exerts a dominant-negative effect...
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Comparatives study on sequence structure function relationship of human short chain dehydrogenases reductases
... indicative of slowly evolving, conserved sites) are divided into 45 equal intervals The score 45 intervals are used for the score above the average (positive values, rapidly evolving, variable sites) ... independently Divergent sequences evolve independently after diverging 3.3.4 Human SDR phylogenetic tree and comparison of consensus sequences The results of our multiple sequence alignments were ... SDR enzymes, a comparative analysis of the 3D protein structure of each of the five consensus sequences was also conducted We identified a representative structure for each of the five groups recovered...
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Investment project analysis of e customs for the authorized economic operator
... by SSL certificate, then sent to receiving party through Internet; at receiving party, MDN message is turned back to sender in order to let the sender acknowledges that receiver already received ... more effective service to customers 9 Possibility to give a chance to improve company market share and competitive advance by having new standard E- Customs system Provide the figure to make a ... same price, service for authorized economic operator brings better benefit than service for normal business based on time, resource and effectiveness perspective 21 Comparing between Demand and...
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Báo cáo khoa hoc:" Failure of E. coli bacteria to induce preterm delivery in the rat" ppsx
... infusion of live E coli fails to induce preterm delivery Our existing mouse model is characterized by a dramatic difference in potency of killed versus live bacteria for induction of preterm delivery ... were observed for various periods of time through one week after delivery to record other observations, such as time to delivery and viability of delivered pups Animals were euthanized by CO2 inhalation ... retention of dye in the uterine horn, continued viability of fetuses (verified by both immediate and delayed necropsy) and minimal leakage out the cervix and vagina No control animals delivered...
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Báo cáo y học: " Lipocalin 2 is protective against E. coli pneumonia" docx
... being used for the experiments The bacteria were harvested, resuspended in PBS, and the suspension of bacteria adjusted to the concentration required for the experiment The titer of the bacteria ... produces siderophores nor use them for iron acquisition but instead have developed other means of iron uptake [23] Infections of the respiratory system by E coli do, however, occur, and may have severe ... The unpaired differences in the continuous data between infected and non-infected mice were analyzed by the Mann-Whitney U-test The software Statview (SAS Institute, Cary, NC) was used for the...
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Báo cáo sinh học: "Comparative expression profiling of E. coli and S. aureus inoculated primary mammary gland cells sampled from cows with different genetic predispositions for somatic cell score" ppt
... that were sired for first parturition in a time interval of six weeks between December 2004 and February 2005 The detailed selection strategy and phenotypes of selected heifers are described by Kühn ... heifers of sire 2, one heifer of sire 3) were assumed to have inherited the paternal chromosomal region decreasing SCS (SCSBTA18-Q) and five heifers (three heifers of sire and one heifer of each ... control cells; the categories are ranked by p-values of the SCS-BTA18-Q and SCS-BTA18-q cells, respectively, and related p-values and the number of involved genes are shown for the alternative QTL...
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Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf
... statistically evaluated by Analysis of Variance (ANOVA), followed by Newman-Keuls-Student’s test P-values of 0.05 or less were considered significant Phospholipase A2 activity PLA2 activity was assayed by ... the PLA2 M-type receptor [47] In both cases, electrostatic interactions between cationic residues on the surface of the myotoxin and negatively charged groups in the membrane seem to be involved ... corresponds to the signal peptide Underlined residues were confirmed by Edman sequencing of DM64 and of CNBr and endoproteinase Lys-C derived peptides The polyadenylation signal is doubly underlined...
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Tài liệu Báo cáo khoa học: Domain IV of mouse laminin b1 and b2 chains Structure, glycosaminoglycan modi®cation and immunochemical analysis of tissue contents pptx
... pyridylethylcysteines and carboxymethylcysteines was estimated from the height of the respective phenylthiohydantoin derivatives in the corresponding sequencer runs Electron microscopy of rotary ... complete reduction under denaturing conditions and carboxymethylation Peptides were then generated by cleavage with endoproteinase Lys-C followed by cleavage with some other proteases and determination ... against the natively folded fragments b 1IV and b 2IV in order to re-examine some of the previous data and to establish more quantitative and sensitive assays The rabbit antisera obtained showed a high...
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Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... avin reductase activity Which structures determine the unique functions of these avin proteins? In this study, we present the crystal structure of DvMF avoredoxin and discuss the key residues ... reductase and HpaC component of Escherichia coli 4-hydroxyphenylacetate 3-monooxygenase Secondary structure elements of avoredoxin are shown on the lines of residue numbers Residues involved in binding ... bridge that encompass the ribitol moiety and the phosphate group of FeR and M acetivorans avoredoxin are moderately and completely conserved in DvMF avoredoxin (Fig 3A,B and Fig S4A), respectively...
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Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx
... phase of exon among all RNase j genes in the available species The above results imply that there has been a highly conservative selective pressure imposed on the RNase j gene structure during evolution, ... demonstrate an expectedly higher degree of variability than the exons, although remarkable similarities exist among several species For example, the higher-vertebrate lineages represented by ... independent intronic deletion may have taken place and led to the two-exon structure seen in the insects N vitripennis and A mellifera As a result of these events, the sum of the sizes exons and...
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