skin tissue engineering part ii — the in vitro evaluation of natural and synthetic 3 d matrices as dermal substrates
Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx
... elucidated, although it has been shown that CRE-decoy treatment reduces cyclin D1 and cyclin-dependent kinase (CDK) levels and retinoblastoma protein (Rb) phosphorylation CDO-induced growth inhibition ... control) with the expected reduction in Fig The effect of CDO in HCT116 and GEO cell lines on day The activity of CDO in the sensitive cell lines was fitted a standard Emax model Representative DNA histograms ... significant differences in the measured level of BrdU incorporation and the expected level (Fig 3) In contrast, there was a significant reduction in BrdU fluorescence in GEO cells cocultured with CDO and...
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Báo cáo khoa học: "Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System" ppsx
... day pi and transferred to a slide in 2 -3 ㎕ of medium, and 15 ㎕ of Hoechst 33 342 stain prepared with sodium citrate (2 .3% ) and ethyl alcohol was added The slide was then incubated on a warming plate ... Reprod 19 93, 48, 132 0- 132 5 12 Lee, E S and Fukui Y Synergistic effect of alanine and glycine on bovine embryos cultured in a chemically defined medium and amino acid uptake by in vitro produced ... M and Okuda, K Effect of phosphate, energy substrates, and amino acids on development of in vitro- matured, in vitro fertilized bovine oocytes in a chemically defined, protein-free culture medium...
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báo cáo khoa học: " In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration" pptx
... for HGF and PDLF, AB solution was added directly in a final concentration of 10% and the plate was further incubated Optical density of the plate was measured at a wavelength of 560/20 and 620/40 ... was to determine optimal seeding density and culture period HGF, PDLF and HOB cells were trypsinized after serum starvation and suspended into standard culture medium with 10% FBS HGF and PDLF ... reported following the use of collagen materials [1] Furthermore, a recent in vitro study has pointed out that native as well as cross-linked membranes derived from bovine or porcine type I and III...
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Báo cáo khoa học: "In vivo and in vitro evaluation of an Acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repair" ppt
... participated in the study design and experimental work SCR participated in the study design, participated in its coordination and drafted the manuscript OCMPJ participated in the study design and experimental ... composition of a membrane intended for guided tissue repair determines the type, duration and degree of inflammatory and immune response, means of disintegration and its longevity in the host tissue ... duraplasty study in dogs the membrane was invaded by connective tissue and membrane filaments had loosened and separated from each other, resulting in its partial disappearance when evaluated...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... values show the MFIs of indicated antigens in DCs B, MFIs of HLA-ABC, HLA-DR, CD80, CD86, and CD 83 in DCs generated before and after vaccination were analyzed C, Fusions of auto-HCC and DCs (before ... resected HCC (obtained with following informed consent and approved by our institutional review board obtained) was isolated and maintained in TIL Media I medium with 10% heat-inactivated FCS, ... regarding the possible induction of hepatitis by this vaccination However, no hepatitis was induced, as evidenced by the constant levels of serum AST and ALT In the present study, vaccination of the...
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Báo cáo y học: " In vitro evaluation of marine-microorganism extracts for anti-viral activity" pot
... formed in 24 hours All medium was removed from the flask and 250 μl of previously made virus stock was mixed with ml of serum-free medium and added into the flask to infect the cells The flasks ... necessary information regarding the origin and preparation of the extracts HTR provided marine isolates for the cultures and extracts YL was the principle investigator of this project and designed and ... conceived of the study, and participated in its coordination, and data analysis and manuscript revision All authors read and approved the final manuscript Competing interests The authors declare...
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Báo cáo khoa học: "In Vitro Evaluation of Frozen-Thawed Stallion Semen: A Review" doc
... 207 of degradation of the plasma membrane as evaluated by FITC-Con-A Addition of glycerol caused significant reductions in VCL and ALH and increased the proportion of damaged spermatozoa, but the ... controlled ( +37 °C) by using a stage warmer on a phasecontrast microscope, the depth of suspension on the slide should be standardized and multiple fields near the centre of the slide examined Motility ... lactic dehydrogenase (LDH), cholinesterase, acid phosphatase and alkaline phosphatase (Brown et al 1971, Risse 1990) AS- ases are present in the acrosome of the intact sperm cell and in seminal plasma...
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Báo cáo sinh học: " In vitro evaluation of a double-stranded selfcomplementary adeno-associated virus type2 vector in bone marrow stromal cells for bone healing" pptx
... CD45 negative and CD90, CD105 and CD146 positive [31 ] (Gift of Pamela Robey, National Institute of Dental and Craniofacial Research, Bethesda, MD USA) were maintained in Minimum Essential Medium ... femurs and the tibias by flushing the medullary canal with Iscove’s Modified Dulbecco’s Medium (IMDM) (Invitrogen, Carlsbad, CA, USA) The cells were maintained in IMDM containing 15% fetal bovine ... wide range of host cell types including dividing and non-dividing cells [20] In addition, there is no conclusive evidence indicating pathogenicity of AAV vector in humans [21] AAV also induces long-term...
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Microbial community structure in anaerobic degradation of terephthalate and phenol 3
... (1 mg/mL) and achromopeptidase (0.1 mg/mL) were added and incubated at 37 ºC for 30 Protease K (0.1 mg/mL) and 1% sodium dodecyl sulfate were added and further incubated at 37 ºC for another hr with ... ethanol, air dried and resuspended in 100 µl of milliQ water (Dnase- and Rnase-free) The obtained DNA was checked for quality and quantity using UV-spectrophotometer at 260 nm and 280 nm and agarose ... Materials and Methods Table 3. 3 lists the oligonucleotide probes used in this study, including EUBmix (i.e., EUB 338 , EUB 338 -II, EUB 338 -III) that targets most of the Bacteria (Amann et al., 1995; Daims...
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Báo cáo toán học: " Dilemmas in the reliable estimation of the in-vitro cell viability in magnetic nanoparticle engineering: which tests and what protocols?" doc
... acetal) [MDA] standard solutions (0 to µM) were prepared, and 100 µL of samples or standards was added to the Eppendorf tubes Sodium dodecyl sulphate (100 µL, 2%) was added, and the tubes were incubated ... upon addition of the PEG moiety [33 ], thus increasing the stability of the nanoparticles and further shielding the cells from the iron oxide core The cationic charge on the PEI is decreased upon ... properties as has been widely reported [35 -38 ] In order to achieve a more in- depth understanding of the degree of cytotoxicity of the MNPs, we also investigated the effect the polymer-coated nanoparticles...
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báo cáo hóa học:" Dilemmas in the reliable estimation of the in-vitro cell viability in magnetic nanoparticle engineering: which tests and what protocols?" potx
... factors including an increase in the coating diameter upon addition of the PEG moiety [33 ], thus increasing the stability of the nanoparticles and further shielding the cells from the iron oxide core ... exclusion was used as the gold standard method to validate the cell viability data obtained by the above assays (Figure 3) This method involved direct counting of viable cells and hence eliminated the ... widely reported [35 -38 ] In order to achieve a more in- depth understanding of the degree of cytotoxicity of the MNPs, we also investigated the effect the polymer-coated nanoparticles had on the...
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Báo cáo y học: "Low self-esteem and psychiatric patients: Part II – The relationship between self-esteem and demographic factors and psychosocial stressors in psychiatric patients" ppt
... Ivani-Chalian C and Robindon M Self-esteem and its sources: stability and change in later life Aging and Society 19 93, 13: 171-192 Brandtstadter J and Greve W Explaining the resilience of the aging self: ... Humain 19 83, 46 :35 5 -36 6 Miller PMcC, Kreitman NB, Ingham JG and Sashidharan SP Self-esteem, life stress and psychiatric disorder J Affect Disord 1989, 17:65-75 29 30 31 32 33 34 35 36 37 38 39 40 ... disorders [39 –41], and alcohol and drug use disorders [42–48] Indeed, it has been proposed that lowered self-esteem is an important etiological determinant in the development of each of these disorders...
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Báo cáo y học: " The effect of interleukin-13 (IL-13) and interferon-g (IFN-g) on expression of surfactant proteins in adult human alveolar type II cells in vitro" docx
... findings were observed in ATII cells cultured in KGF (K) alone, in d of K and d of KD (data not shown) or in d of KIA and d of KIAD (Additional File 1A and 1B) The major difference was that in ... (Additional File 1A, B, Additional File 2) In all conditions tested, IL- 13 reduced the levels of proSP-C and mature SP-C and IFN-g increased the level of proSP-C Hence, the effects of IL- 13 and ... inhibition of cathepsin H results in decreased production of mature SP-B Napsin A and pepsinogen C, asparatic proteases, are also involved in the processing of proSP-B [ 53, 63] We found no changes in the...
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In vitro and in vivo evaluation of customized polycaprolactone tricalcium phosphate scaffolds for bone tissue engineering
... preparation and analysis 32 v 3. 2 .3. 5 Molecular weight testing 3. 2.4 Statistical analysis 33 33 3. 3 RESULTS 34 3. 3.1 Porosity measurements and 3D model analysis 34 3. 3.2 Weight loss analysis 36 3. 3 .3 Compressive ... reported that within the Lipase PS-PCL system, the degradation rate was mainly dependent on the first step [Gan, 1999] In addition, the amount of degradation and the degradation rate of PCL depended ... PREFACE The thesis is submitted for the degree of Master of Engineering in the Department of Mechanical Engineering at the National University of Singapore under the supervision of Professor...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... 1. 13 ã 10)2 s)1 (D) (E) shows a plot of the original and the updated model with the inclusion of newly measured Ki in the updated model amplitudes are damped) We have thus far demonstrated that ... antibody and Draq5 dye; or and Hoechst 33 258 dye) was dispensed into each well and left to incubate for h at room temperature in the dark The plates were inverted to remove the antibody solution and ... IKK2 inhibitor) of IL-1a was prepared for cell assay and was added following the same procedure described for the time course cell assay The cells were washed with NaCl Pi and xed after 400 min,...
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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc
... PvuII-linearized pCOBsal DNA template was 632 nucleotides (nt) in length and contained 31 1 nt of intron sequence, 112 nt of 5¢ exon and 209 nt of 3 exon sequence HindIII-derived pre-RNAs contained ... called I-AniI One other in vitro maturase-assisted splicing assay has been developed recently for the yeast mitochondrial bI3 intron ORF, but in this interesting case, the maturase requires the assistance ... quantified in the same way All pre-RNAs were derived from PvuII-linearized DNA templates except for indicated experiments presented in Fig 5C ,D where HindIII-linearized DNA templates were used [33 ]...
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Báo cáo khoa học: The in vitro nuclear aggregates of polyamines pot
... persuaded that the in uence of NaCl in determining the size and shape of the aggregates is 233 0 quite delicate, and needs to be investigated in detail Dynamic light scattering (DLS) measurements ... DNA was isolated from peripheral blood leukocytes DNA was extracted and purified using a standard phenol ⁄ chloroform procedure, and then resuspended in Tris ⁄ EDTA buffer The in vitro self-assembly ... preincubated with 1.25 lg of genomic DNA and H2O in a final volume of 20 lL for 10 at 37 °C Subsequently, 100 ng of EtBr was added, and the solution was gently vortexed and incubated in the dark...
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báo cáo hóa học:" In vitro suppression of the MMP-3 gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA" doc
... action in chondroprotection [38 ] Our findings indicated that MMP -3 siRNA specifically decreased the expression of the MMP -3 gene, and led to decreased cell apoptosis, and increased cell viability and ... penicillin and 100 μg/ml streptomycin (GIBCO® Invitrogen), and then cultivated in a CO2 incubator (5% CO2, 37 °C) When the cells had reached confluence, the media was removed and the cells washed in ... (DMMB) assay for sulfated glycosaminoglycan 10 using chondroitin sulfate C (shark cartilage extract; Sigma-Aldrich, USA) as standard The DMMB solution was added to the diluted sample and standard...
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báo cáo hóa học:" The effect of abductor muscle and anterior-posterior hip contact load simulation on the in-vitro primary stability of a cementless hip stem" ppt
... presented in Figure 2) group doff was 32 mm, whereas in group it was set at 0. 83* dm, (and since dm ranged between 46 and 49 mm, doff therefore ranged between 38 and 41 mm) The Fap was applied in three ... participated in the introduction and study design GF provided important feedback on the experimental setup and partipated in the discussion HF provided the design of the experimental setup and participated ... Axial and torsional loading of the actuator produced distal (Fd), medial (Fm) and anterior-posterior (Fap) loading of the femoral head due to the mounting geometry and the offset between the femoral...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25