role of sa in the regulation of cadmium toxicity in maize zea mays l
báo cáo khoa học: " The PTI1-like kinase ZmPti1a from maize (Zea mays L.) co-localizes with callose at the plasma membrane of pollen and facilitates a competitive advantage to the male gametophyte" doc
... component of the pollen grain and pollen-tube wall but also plays a key role in pollen viability Callose is deposited in the inner wall layer behind the growing pollen tube tip as well as in regularly ... generative cell (left); bright field image of the same pollen (right) (J) Trinucleate pollen of wild type line H99 stained with decolorized aniline blue and showing callose deposition as annulus-ring ... During the time interval when the generative cell abuts against the wall, callose occurs at the boundary of the cell, however, only on the side adjacent to the vegetative cell This is called the...
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Báo cáo y học: "Toxicogenomic analysis of Caenorhabditis elegans reveals novel genes and pathways involved in the resistance to cadmium toxicity" potx
... and control C elegans were labeled using Low RNA Input Fluorescent Linear Amplification kits following the manufacture's protocols (Agilent Technologies, Inc, Santa Clara, CA, USA) Labeled cRNAs ... cadmium toxicity (although these genes certainly could also be involved in detoxification of other stressors) These cadmium resistance genes include previously known genes involved in the cadmium ... various genes in cadmium related diseases in humans Materials and methods C elegans strains The following strains were used in this study: N2 Bristol; mtl2 null, mtl-2 (gk125); mtl-1 null, mtl-1 (tm1770);...
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báo cáo khoa học: "Structure and expression of the maize (Zea mays L.) SUN-domain protein gene family: evidence for the existence of two divergent classes of SUN proteins in plants" pot
... difficult, at least partly because the anther is made up of several different cell types that include not only cells in meiosis but also a layer of epidermal, intermediate, and tapetal cells The expression ... cells, shown in Figure revealed the strongest staining around the nuclear periphery but also detected considerable speckled cytoplasmic staining in a postmeiotic uninucleate pollen mother cell ... Perinuclear space Nucleoplasm Figure 10 Maize SUN topology models relative to the membranes of the nuclear envelope Possible protein arrangement models with the SUN (yellow) domain in the perinuclear...
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Tài liệu Báo cáo Y học: The effects of low pH on the properties of protochlorophyllide oxidoreductase and the organization of prolamellar bodies of maize (Zea mays) pot
... assay medium at pH 7.5 (A) The initial sample (thin line); aged sample before (thick line) and after (medium line) exposure to light (B) Illuminated sample before (thin line) and after (thick line) ... phosphorylation step is involved in pigment loading The precise role of any possible POR kinase therefore still remains unclear The main line of evidence for the existence of a PORphosphatase is the observation ... are all clearly visible in the difference spectra shown in Fig Changes in the wavelength of the Chlide absorption maximum following photoconversion of washed PLB samples suspended in pH 7.5 assay...
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Rapid transcriptome responses of maize (Zea mays) to UV-B in irradiated and shielded tissues Paula Casati and Virginia Walbot pptx
... candidate molecule participating in UV-B signaling, as it is also involved in signaling in plants [37] Genes for protein degradation are also upregulated in UV-B-shielded leaves Finally, several transcripts ... while RAD17 is induced in seedling and adult leaves These proteins are involved in post-replication repair of DNA in yeast, and they activate checkpoints that delay cell-cycle progression in ... Article R16 Casati and Walbot shielded tissues exhibit upregulation of ribosomal protein genes Because seedling leaves lack both the downregulation of photosynthetic genes and upregulation of ribosomal...
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báo cáo khoa học: " Characterization of phenylpropanoid pathway genes within European maize (Zea mays L.) inbreds" ppsx
... previously assumed Methods Plant materials and phenotypic analyses A collection of 40 maize inbred lines consisting of 22 Flint and 18 Dent lines were included in this analysis The line collection ... 1-bp indel in 4CL1 was found to be associated with IVDOM by both GLM and MLM The insertion allele of this indel is present in only one line, AS18, which exhibits the second lowest overall value of ... with an increase in cell wall digestibility For the COMT gene, indel polymorphisms in the intron region have been associated with cell wall digestibility in two different sets of lines, one of which...
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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf
... insights into the association of other cell cycle proteins with the promoter E2F4 binding during the cell cycle coincides with the binding of p107 or p130 [61,85] Another E2F site distal to the ... compared with wild-type cells when assayed using in vivo footprinting Loss of the two pocket proteins leads to deregulation of the B-Myb promoter during the cell cycle Re-introduction of p107, and ... adopt the timing of expression from the other promoter as a result of replacing the cell cycle-regulatory elements Thus, it is likely that cell cycle-dependent timing of expression is also determined...
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Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt
... biologically relevant role of miR-27 in the regulation of adipose tissue functions in vivo, we examined the expression of miR-27 in the genetically obese ob ⁄ ob mice The 2352 expression levels ... Rockville, MD, USA) and maintained in the culture conditions recommended by the ATCC Briefly, 3T3 -L1 cells were cultured in DMEM containing 10% fetal bovine serum OP9 cells were grown in aMEM containing ... may play a role in the regulation of adipose functions associated with obesity Results miR-27 inhibits adipogenic differentiation In order to investigate the role of miRNAs in the regulation of...
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Báo cáo khoa học: Use of lithium and SB-415286 to explore the role of glycogen synthase kinase-3 in the regulation of glucose transport and glycogen synthase pdf
... Consequently, whilst PP1G is likely to play a significant role in the hormonal activation of GS in 3T3 -L1 adipocytes, the importance of GSK3 in the insulin-mediated regulation of this enzyme in fat cells ... establish the importance of GSK3 in the acute regulation of glucose transport in terms of its regulatory control of GS in muscle and fat cells we have investigated the effects of Li and the anilinomaleimide, ... belief that PI3K plays a critical role in the hormonal regulation of glucose transport [1] However, despite the fall in basal glucose uptake the net stimulation in glucose uptake elicited by Li...
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Báo cáo khoa học: Regulation of luteinizing hormone receptor mRNA expression by mevalonate kinase – role of the catalytic center in mRNA recognition potx
... mobility shift analysis was performed using [32P]-labeled LBS with all the double mutants in the absence and presence of a 10-fold molar excess of wild-type LBS and mutant LBS in which all the ... (Fig 4) The role of these amino acids in LHR mRNA binding FEBS Journal 275 (2008) 3397–3407 ª 2008 The Authors Journal compilation ª 2008 FEBS A K Nair et al Active site of LRBP involved in LHR mRNA ... the site of ATP binding and may exploit common side chain interactions; or (b) the conformations of residues involved in RNA binding may be directly or indirectly coupled to the binding of ATP...
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Báo cáo khoa học: Alternative initiation of transcription of the humanpresenilin 1gene in SH-SY5Y and SK-N-SH cells The role of Ets factors in the regulation ofpresenilin 1 pptx
... extents in each cell type Hence it is likely that Ets elements play a major role in determining both the level of expression and the location of the start of transcription of PS1 In the Ets family of ... tenfold in SK-N-SH and cells Further deletion to +42 increased transcription in both cells lines: about fivefold in SH-SY5Y cells and twofold in SK-N-SH cells Further deletions to +6 did not result ... crucial role in the regulation of other genes [34] Ets factors are likely to be important determinants in the choice of initiation site in a particular cellular context The specificity of Ets...
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Báo cáo khoa học: 2-Oxo acid dehydrogenase complexes in redox regulation Role of the lipoate residues and thioredoxin pot
... within the volume of the enzyme complexes may result in unusual properties of the compound, when compared to the same quantity of the lipoate molecules distributed in bulk solution In particular, ... neighbouring sulfur greatly increases the stability of the thiyl radical in dithiols compared to monothiols [29] Additional stabilization of the lipoate thiyl radicals may be expected within the network ... with the three lipoyl domains cannot be ascribed entirely to the catalytic role of these domains Rather, the advantage appears to depend on the ability of the lipoyl group to protrude from the inner...
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Báo cáo Y học: Elucidation of the role of fructose 2,6-bisphosphate in the regulation of glucose fluxes in mice usingin vivo 13 C NMR measurements of hepatic carbohydrate metabolism docx
... signals of glycogen in the intact liver in vivo Second, during infusion of [1-13C]glucose, label incorporation was observed not only into the C1 of glycogen but also the C6, which can only occur ... intraperitoneally as an alternative means of glucose administration should the tail vein fail, which was the case in only three animals, the data of which were included After the lines were inserted, ... Glc6P6 Measurement of the rate of into glycogen in the liver 13 C label incorporation Label incorporation into the glycogen C1 reflects glycogen synthesis via the direct pathway (glucosefiglucose-6-phosphatefiglycogen),...
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Explaining Bank Failures in the United States: The Role of Self-Fulfilling Prophecies, Systemic Risk, Banking Regulation, and Contagion pdf
... speculation and self-fulfilling prophecies trigger actual bank failures Based on the coefficients of the baseline model of column of table 1, the solid line of figure represents the predicted number of ... percent of bank failures within the total sample, but also leaves profound developments in financial services liberalization, which included in particular the deregulation of branching restrictions, ... estimates The decade following 1982 was marked by a dramatically aggravating degree of fragility in the banking industry culminating in the savings and loans (S &L) crisis After dropping the 1982 to 1992...
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AN INCREASING ROLE FOR COMPETITION IN THE REGULATION OF BANKS doc
... development of offshore financial centers and off-balance-sheet methods of financing); • the diminishing effectiveness of traditional controls due to financial innovation (including the difficulty of ... “S &L crisis” in the US of the early 1980s In the case of S&Ls the insurance premium was set by statute in 1950 at 1/12 of 1% of the assessable deposits and was the same for all insured institutions ... financial products is hindered by lagging regulatory developments, delaying and stifling the pace of innovation 40 Partly as a result of an increasing recognition of these problems, the Basel Accord...
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báo cáo sinh học:" The role of regulation in influencing income-generating activities among public sector doctors in Peru" doc
... highlighted the role of regulation in reconciling the collective interests within the medical profession with broader policy objectives such as maintaining staff in the public sector and quality control ... jobs to supplement their income "In the past 10 years the classical form of dual practice of a public job in the morning, doctor's office in the afternoon, has been declining; the multi-job practice ... practice There are an increasing number of newly graduated professionals: "In Lima alone there are seven medicine faculties and still growing" (General Medicine #3, private clinics) "The insurance...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pptx
... 5ng/ml FGF Page of 5% Normal Media + 50ng/ml BMP 5% Mineralizing Media + 50ng/ml BMP Figure Alizarin Staining of Mineralizing Osteoblast cells MC3T3-E1 osteoblasts were seeded at 3000 cells/well in ... to heal the defect The increase in cell replication in a mineralizing cell likely represents a de-differentiation from the mineralizing phase to the growing phase, and increases expression of GFs ... sarkosyl lysing buffer was added to each well; all the cells were solubilized after 30 minutes Finally, after mixing the resulting solution with a pipette, radioactivity was counted in a scintillation...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pdf
... 5ng/ml FGF Page of 5% Normal Media + 50ng/ml BMP 5% Mineralizing Media + 50ng/ml BMP Figure Alizarin Staining of Mineralizing Osteoblast cells MC3T3-E1 osteoblasts were seeded at 3000 cells/well in ... to heal the defect The increase in cell replication in a mineralizing cell likely represents a de-differentiation from the mineralizing phase to the growing phase, and increases expression of GFs ... sarkosyl lysing buffer was added to each well; all the cells were solubilized after 30 minutes Finally, after mixing the resulting solution with a pipette, radioactivity was counted in a scintillation...
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Báo cáo y học: " Tumour necrosis factor-α stimulates dehydroepiandrosterone metabolism in human fibroblast-like synoviocytes: a role for nuclear factor-κB and activator protein-1 in the regulation of expression of cytochrome p450 enzyme 7b" doc
... to play a role in RA, including extracellular signal (mitogenic)-regulated protein kinase (ERK)1/2; the stress-activated protein kinases, also called cJun NH2-terminal kinases (JNKs); and the ... Similar findings were obtained using five additional RA FLS cell lines; a small inhibitory effect of the p38 inhibitor SB203580 at high concentration (1 × 10-5 mol /l) was observed in one cell line ... Importantly, the increase in Cyp7b activity following stimulation of the cells with TNF-α was dose-dependently inhibited by SN50 (Fig 1a) To further substantiate this finding, five other FLS cell lines...
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Báo cáo y học: "The role of codon selection in regulation of translation efficiency deduced from synthetic libraries" potx
... S1 in Additional file 2), excluding the possibility of examining the effect of the proximal bottleneck on the expression of these two proteins Nonetheless, we could still compute the correlation ... genes in the GFP library The x-axis is the relative location of the bottleneck in every gene; the y-axis is the per-cell protein abundance The color of each dot is the relative strength of the ... not only in the levels of expressed protein from a gene but also in the cost of expression Considering the cost of production, we looked at how introducing a new gene into the host cell influenced...
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