role of oxidative stress and antioxidants in male infertility
Báo cáo khoa học: Effect of oxidative stress and involvement of poly(ADP-ribose) polymerase (PARP) in Dictyostelium discoideum development doc
... PARP inhibitor, on oxidative stress- induced changes in D. discoideum development. Interestingly, oxidative stress resulted in PARPactivation within 5 min that was inhibited by benzamide. Oxidative ... development.HA-induced oxidative stress activates PARP within5 min (Fig. 4A,B), and its role during oxidative stress is further confirmed by the use of low concentrations of benzamide. Preincubation of cells ... the response of this organism to oxidative stress and the role of PARP during development. We used hydroxylamine (HA)to induce in situ generation of H2O2 and monitored the effect of benzamide,...
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Báo cáo khoa học: The role of residues R97 and Y331 in modulating the pH optimum of an insect b-glycosidase of family 1 pdf
... Nonretained proteins were washed out with 20 mMtriethanolamine buffer pH 8.0 and retained proteins wereeluted using a gradient of NaCl prepared in the same initialbuffer. The presence of the ... clearly increased. Actually, the substitution of R97 bymethionine or lysine resulted in the same increment in thepKa of E399 (Table 2). Therefore, M97 and K97 are equallyeffective in stabilizing ... toE187, in order to minimize unfavourable interactions withthe apolar side chain of F331. Thus, the increment in thepKa of E399, in addition to it being closer to E187, mayhave resulted in a...
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Báo cáo hóa học: " An exploration of job stress and health in the Norwegian police service: a cross sectional study" docx
... acquisition,analysis and interpretation of data and drafting of themanuscript. EH was involved in design, interpretation of data, drafting of the manuscript and supervision. ØE wasinvolved in conception and ... Brown J, Fielding J, Grover J: Distinguishing traumatic, vicarious and routine operational stressor exposure and attendantadverse consequences in a sample of police officers. Work Stress 1999, ... wasinvolved in conception and design, interpretation of data,drafting of the manuscript and supervision. BL wasinvolved in analysis and interpretation of data. AMB is theguarantor for this...
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Ambient particulate air pollution induces oxidative stress and alterations of mitochondria and gene expression in brown and white adipose tissues ppt
... significant insights into the roles and mechan-isms of PM2.5-associated physiology and pathology. In summary, our data demonstrate the importanteffects of PM2.5exposure on oxidative stress and ... effect of ambient PM2.5onWATandBATspecificgenesindifferent adipose depots. There are several major find-ings in this study. First, exposure to PM2.5resulted in oxidative stress in BAT. ... blot.A.RepresentativebandsofFAandPM2.5onUCP1 protein level in iBAT by Western blotting. B. Quantitative results of Western blotting of UCP1. n = 8. *P<0.05 vs. FA.Xu et al. Particle and Fibre Toxicology...
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Báo cáo khoa học: Oxidative stress is involved in the permeabilization of the inner membrane of brain mitochondria exposed to hypoxia/reoxygenation and low micromolar Ca2+ Lorenz Schild1 and Georg Reiser2 doc
... that the mitochondrial injury is caused by open-ing of the mPTP. There is evidence that ADP inhibitsopening of the mPTP by occupying binding siteslocated in the inner and outer mitochondrial ... reperfusion in brain.There is a growing body of information concerningthe mechanism of ROS generation by the respiratorychain in mitochondria. Complex I and complex IIIwere identified as generators of ... and oxygen consumptionwas analysed. The corresponding values are shown in Fig. 1. After 15 min of incubation in the presence of 3.5 lm Ca2+ in an air atmosphere, state 3 respiration of brain...
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Báo cáo khoa học: Expression of CYP2E1 increases oxidative stress and induces apoptosis of cardiomyocytes in transgenic mice pdf
... A rapid and sensitive method forthe quantitation of microgram quantities of proteinutilizing the principle of protein-dye binding. AnalBiochem 72, 248–254.CYP2E1 causes apoptosis of cardiomyocytes ... College, Beijing, China2 Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Institute of Laboratory AnimalScience, Chinese Academy of Medical ... overexpression of CYP2E1 both in vivo and in vitro is associated with several cellular markers of oxidative stress, including products of lipid peroxi-dation (e.g. 4-hydroxynonenal and malondialdehyde),as...
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Báo cáo khoa học: The silencing of adenine nucleotide translocase isoform 1 induces oxidative stress and programmed cell death in ADF human glioblastoma cells doc
... [41], and human U87-MGcell line, a kind gift of Professor C. Martini (Department of Psychiatry, Neurobiology, Pharmacology and Biotechnol-ogy, University of Pisa, Italy), were maintained under ... by trypsin treatment and stained for 30 min at 37 C in fresh medium containingJC1 (nal concentration of 10 lgặmL)1). Cells were thenwashed twice with PBS, suspended in 500 lL of PBS and analyzed ... ultrastructural evidence of membrane rup-ture and were also negative to propidium iodide stain-ing (data not shown), indicating that the plasmamembrane is maintained intact, allowing us to excludeprimary...
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Tài liệu Báo cáo khoa học: Role of hydroxyl group and R/S configuration of isostere in binding properties of HIV-1 protease inhibitors docx
... onconformation of inhibitor main chain. Thus, differentinhibitors differ not only in t he binding affinity, bu t a lso in the degree o f freedom of the inhibitor inside the bindingtunnel. Some s pecial inhibitors ... foundrotatedtoeachother.TheanglesbetweenplainsofP3Âphenyl rings are 98 for OE and SE inhibitors, and 38 forOE and RE inhibitors. The S3Â groups of OE and REinhibitors having a similar orientation phenyl rings form asimilar ... of projections of opposite Sdatoms of M et46 and Met146).Ó FEBS 2004 HIV-1 Protease Inhibitors (Eur. J. Biochem. 271) 4457 Inhibitor binding. The inhibitor OE b inds into the p roteasebinding...
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Tài liệu Báo cáo khoa học: Enhancement of oxidative stress-induced apoptosis by Hsp105a in mouse embryonal F9 cells pptx
... determine theeffects of Hsp105a on JNK and p38 signalling pathways, weexamined the activation of JNK and p38 in F9 cells by oxidative stress. As shown in Fig. 6A, JNK was notactivated in control ... activation of p38 induced by oxidative stress, we further examined whether its activationis responsible for the induction of apoptosis in F9 cells usingSB202190, a potent inhibitor of p38. As shown in ... peroxide. In contrast, hydrogen peroxide-treat-ment induced marked activation of JNK within 30 min in S23 cells, but not in S3 cells. Therefore, the enhancement of the oxidative stress- induced...
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Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
... the binding of GTP to the allosteric site promotes coordination of thephosphorylation of UTP and hydrolysis of glutamine foroptimal efficiency in CTP synthesis rather than just acting toincrease ... kinetics of the glutaminase reaction of CTP synthasefromLactococcus lactisThe role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesisMartin Willemoeăs1 and ... role for GTP activation of CTP synthesis. A mechanism in which GTP only acts to increase the rate of hydrolysis of glutamine has some flaws in explaining the inhibition of DON-labeledenzymewhenassayedwithNH4Cl...
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