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responses of lactic acid bacteria to oxidative stress

The roles of lactic acid bacteria in host inflammatory responses

The roles of lactic acid bacteria in host inflammatory responses

Cao đẳng - Đại học

... immune responses upon a variety of stimuli, including lactic acid bacteria (LAB) LAB are a group of related bacteria that produce lactic acid as a result of carbohydrate fermentation Lactobacilli, ... Tumor growth factor Th - T helper cell TLRs - Toll like receptors TNF - Tumor necrosis factor Tollip - Toll inhibitory protein TRAF6 - TNF receptor associated factor TSA - Tryptone Soy Agar U/ml ... THE ROLES OF LACTIC ACID BACTERIA IN HOST INFLAMMATORY RESPONSES WANG SHUGUI (B.Sc., Sun Yat-Sen University) A THESIS SUBMITTED FOR THE DEGREE OF PHILOSOPHY OF DOCTORATE DEPARTMENT OF MICROBIOLOGY...
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Analysis and practical implementation of a model for combined growth and metabolite production of lactic acid bacteria

Analysis and practical implementation of a model for combined growth and metabolite production of lactic acid bacteria

Báo cáo khoa học

... the total amount of lactic acid in the medium and the buffering capacity of the medium are known The amount of undissociated lactic acid is then further related to the total amount of lactic acid ... filtration of the sample to remove the cells (Sarstedt, pore size 0.2 mm) and derivatisation of the lactic acid present in the filtrate with methanol, lactic acid was measured through gas chromatographic ... theoretical analysis of the model Some mathematical properties of the model are discussed, and an easy -to- use method to take into account the evolution of pH and undissociated lactic acid [LaH] in the...
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Probiotic potential of lactic acid bacteria isolated from chicken gastrointestinal digestive tract pot

Probiotic potential of lactic acid bacteria isolated from chicken gastrointestinal digestive tract pot

Ngư nghiệp

... general, the acid tolerance of LAB depends on the pH profile of H?-ATPase and on the composition of the cytoplasmic membrane This is largely influenced by the type of bacterium, the type of growth ... bile acids are less inhibitory than free bile acids (cholic and deoxycholic acid, DCA) toward intestinal aerobic and anaerobic bacteria Taurine-conjugated deoxycholic acid (TDCA) was less toxic ... are evaluated as beneficial bacteria by their product of acids (lactic acid) , bacteriocin or bacteriocin-like substances Putrefactive bacteria are regarded as harmful bacteria in that they decompose...
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Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf

Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf

Báo cáo khoa học

... al Storz G & Zheng M (2000) Oxidative stress In Bacterial Stress Responses (Storz, G & Hengge-Aronis, R, eds), pp 47–59 ASM Press, Washington, DC Touati D (2000) Iron and oxidative stress in bacteria ... role of Bcp2 in order to increase the knowledge of the enzymatic activity involved in the oxidative stress in S solfataricus To detect differences in the response to various agents we induced oxidative ... presence of an electron donor, such as dithiothreitol (DTT), Fe3+ catalyses the reduction of O2 to H2O2, which is further converted to OH by the Fenton reaction [18] The MCO system causes damage to...
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Báo cáo khoa học:

Báo cáo khoa học: "Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use" ppsx

Báo cáo khoa học

... bacterial culture Isolation of cholesterol-lowering lactic acid bacteria 393 Table Number of acid- and bile-tolerant lactic acid bacteria isolated from the human intestine for probiotic use Acida ... Lactobacillus HJL-4 Lactobacillus HJL-20 Lactobacillus HJL-24 Lactobacillus HJL-25 Lactobacillus HJL-30 Lactobacillus HJL-32 Lactobacillus HJL-33 Lactobacillus HJL-34 Lactobacillus HJL-37 Lactobacillus ... benefits from lactic acid bacteria FEMS Microbiol Rev 1990, 7, 175-188 11 Gilliland SE, Walker DK Factors to consider when selecting a culture of Lactobacillus acidophilus as a dietary adjunct to produce...
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Báo cáo lâm nghiệp:

Báo cáo lâm nghiệp: "Response to an ozone gradient of growth and enzymes implicated in tolerance to oxidative stress in Acer saccharum (Marsh.) seedlings" pot

Báo cáo khoa học

... stress was too large and possibly exceeded the capacity for detoxication and repair of the neoformed ush The defensive capacity of these neoformed ush seems to collapse at the end of the season ... (2) the response of major enzymes of the catabolic pathways of sugar maple seedlings exposed to an extensive gradient of O3 , from to 300 nL.L1 MATERIAL AND METHODS 2.1 Growth of the seedlings ... referred to as episodic seedlings The rst ush of leaves will be hereafter referred to as the preformed ush, consisting of preformed leaves and the second ush as neoformed ush consisting of neoformed...
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Báo cáo y học:

Báo cáo y học: "Anti-inflammatory potential of a malleable matrix composed of fermented whey proteins and lactic acid bacteria in an atopic dermatitis model" pot

Báo cáo khoa học

... of inflammatory cells following a direct topical contact [11] In addition, some Lactic Acid Bacteria (LAB) have shown immunomodulatory and anti-inflammatory activities The genus Lactobacillus ... amplification of inflammatory reaction [46] The reduction of this pro-inflammatory cytokine following MPM consumption indicated its potential in the inhibition of development of inflammatory disease ... measured every day Page of 10 (page number not for citation purposes) Journal of Inflammation 2007, 4:6 Prophylactic protocol – Mouse ACD The prophylactic anti-inflammatory potential of MPM was evaluated...
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The roles of shca proteins in response to oxidative stress 1

The roles of shca proteins in response to oxidative stress 1

Cao đẳng - Đại học

... nuclei 30 10 30 cytosol Figure 3.4E Phosphorylation of FOXO3a at Thr32 induced by H O involved ERKs activation H O treatment led to translocation of FOXO3a from the nucleus to the cytoplasm Cells ... H2O2 for different periods of time, harvested, and separated to the nuclear and cytoplasmic fractions The levels of FOXO3a, TopBP1 (nucleus control), and p66shcA (cytoplasm control) were determined ... expression of p27 and its role in oxidative stress response (A) H2O2 treatment down-regulated expression of p27 without affecting MnSOD Cells were treated with 0.1–0.5 mM of H2O2 for 20 h The levels of...
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The roles of shca proteins in response to oxidative stress 2

The roles of shca proteins in response to oxidative stress 2

Cao đẳng - Đại học

... expressed both p52 and p46 Shc due to the use of two start codons Bottom panel: quantitation data from B The value of co-immunoprecipitated p52Shc or p46Shc in the absence of H2O2was set at -95- C IP: ... 10 total lysate 2.5 10 particulate 2.5 10 cytosol Fig 4.4 Cell fractionation studies of PKC δ and ShcA NIH3T3 cells were treated with H2O2 for 2.5, 5, 10 mins, collected and separated into cytosolic ... p52ShcA construct did not express p46 due to a point mutation Bottom panel: quantitation data from C The value of co-immunoprecipitated PKC δ in the absence of H2O2 was set at “-” : no H2O2; “+”...
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fermentation of mango juice by lactic acid bacteria

fermentation of mango juice by lactic acid bacteria

Tổng hợp

... value of fruit juice fermented by lactic acid bacteria (LAB) Four isolates of lactic acid bacteria from mango were obtained The results of research showed that all 10 strains of lactic acid bacteria ... isolates were able to survive and grow at this pH The cause of the decrease in pH was due to the development of lactic acid bacteria that produced lactic acid Content of lactic acid produced from ... products stored at - 60C and content of lactic acid bacterial density almost stable after weeks of storage Content of lactic acid from 1.07 to 1.1 %w/v and bacterial density 8.51 20 8.16 (log...
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fermentation of carrot juice by lactic acid bacteria

fermentation of carrot juice by lactic acid bacteria

Tổng hợp

... Isolation of lactic acid bacteria - Study on low pH tolerance of isolates - Study on lactic acid fermentation ability of isolates in carrot juice - Identification of the selected lactic acid bacteria ... To isolate and select lactic acid bacteria and to study the favorable conditions of lactic acid fermented carrot juice with biological values The content of this study includes: - Isolation of ... Isolation of lactic acid bacteria 3.2 Study on low pH tolerance of isolates 3.3 Study on lactic acid fermentation ability of isolates in carrot juice 10 3.4 Identification of the...
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fermentation of dragon fruit juice by lactic acid bacteria

fermentation of dragon fruit juice by lactic acid bacteria

Tổng hợp

... organic acids,… Dragon fruit juice is a suitable medium for lactic acid bacteria to produce new probiotic products Therefore, the study “Fermentation of dragon fruit juice by lactic acid bacteria ... isolated bacteria strains belonged to lactic acid bacteria because they: - Are Gram-positive bacteria - Has no enzyme catalase - Are rod-shaped or streptococcus - Can grow on MRS medium 3.2 Acidity tolerant ... concentrations of bacteria began to reduce due to the inhibition of acid against bacteria (less than 6.0 log cells/mL) Thus, these temperatures were not suitable for storing products The samples stored...
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fermentation of pineapple juice by lactic acid bacteria

fermentation of pineapple juice by lactic acid bacteria

Tổng hợp

... for lactic acid bacteria to produce new probiotic products Therefore, the research “Fermentation of pineapple juice by lactic acid bacteria was carried out Objectives To isolate lactic acid ... hours because of converting sucrose to lactic acid by LAB L acidophillus from Ybio probiotic powder had a preponderance of lactic acid content LAB density at initial inoculum was close to inoculum ... sugar into lactic acid inhibited the bacterial growth and development Besides, lactic acid itself could inhibit the development of bacteria (Nguyen Thi Hien, 2006) In contrast, the treatment of 15...
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Tài liệu Báo cáo khoa học: Metabolic gene switching in the murine female heart parallels enhanced mitochondrial respiratory function in response to oxidative stress pdf

Tài liệu Báo cáo khoa học: Metabolic gene switching in the murine female heart parallels enhanced mitochondrial respiratory function in response to oxidative stress pdf

Báo cáo khoa học

... expression of peroxisome proliferator-activated receptor-alpha (PPARa) and peroxisome proliferator-activated receptor-gamma coactivator-1 (PGC-1), pivotal nuclear regulators of numerous fatty acid ... main finding of this study is the identification of a metabolic gene switch from fatty acids to glucose in the murine female heart at baseline linked to enhanced mitochondrial respiratory capacity ... mitochondrial respiratory function in female control mouse hearts in response to oxygen lack (A) Percentage recovery of state respiration after 20 of oxygen deprivation (B) Total postanoxic mitochondrial...
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Physiological adaptability of three mangrove species to salt stress ppt

Physiological adaptability of three mangrove species to salt stress ppt

Nông nghiệp

... rate and stomata conductance of Sa and Sc were relatively stable, which showed the adaptation of the two species to low salinity Net photosynthesis rate and stomata conductance of Rs rose to the ... salt tol- nonstomatal components of photosynthesis and dissipation of erance and induces biosynthesis of both mannitol and a gluco- excess light energy Planta, 1992, 187: 335–347 syl-mannitol ... photosynthesis rate of Sa, Sc and Rs Results and analysis 2.1 Effects of salinity on leaf net photosynthesis rate, stomata conductance and transpiration rate of Sa, Sc and Rs The indices of leaf...
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Báo cáo sinh học:

Báo cáo sinh học: "Metabolic reconfiguration is a regulated response to oxidative stress" pdf

Báo cáo khoa học

... powerful tool to show that altering this redox balance to a more oxidized state causes sensitivity to oxidative stress Expressing K lactis Gdp1p in S cerevisiae also impaired the oxidant tolerance ... oxidant that promotes the formation of disulphides It reacts rapidly and spontaneously with glutathione to cause oxidative stress This finding was extended [3] to show a remarkable correlation between ... pentose phosphate pathway (glucose 6-phosphate dehydrogenase, G6PDH) removed the resistance to oxidants, confirming the role of the pentose phosphate pathway in the TPI-dependent oxidant tolerance...
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Báo cáo lâm nghiệp:

Báo cáo lâm nghiệp: "Growth versus storage: responses of Mediterranean oak seedlings to changes in nutrient and water availabilities." ppsx

Báo cáo khoa học

... ontogeny and resource availability We expected the reduction of seedling [St] with fertilization to be a consequence of higher carbon consumption by growth (hyptothesis 1), but the lack of DM responses ... as water stress limits both carbon and nitrogen gain [42] The aim of the present study was to assess the combined response of growth, carbohydrate and nitrogen reserves of seedlings of three ... species followed a clear Stpool -[St] trajectory in response to an increase of water Oak seedlings responses to nutrient and water 207 Figure Effects of fertilization (left) and watering (right)...
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Báo cáo toán học:

Báo cáo toán học: "Responses of two Populus clones to elevated atmospheric CO concentration in the field 2" ppsx

Báo cáo khoa học

... of total leaf area to total leaf biomass Leaf area index was estimated from total leaf area per plant and number of plants per clone and per ground area of the ) ring (50 m Stem diameters of ... day The height of the plenum may be increased by means of extensive legs This has permitted us to follow the growth of plants and allowed for CO fumigation of the plant canopy up to m in height ... for growth and survival of plants Osmotic adjustment in leaves of plants exposed to elevated [CO due to enhanced con] centrations of soluble sugars [3] might allow them to maintain higher relative...
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The rheumatoid arthritis shared epitope increases cellular susceptibility to oxidative stress by antagonizing an adenosine-mediated anti-oxidative pathway ppt

The rheumatoid arthritis shared epitope increases cellular susceptibility to oxidative stress by antagonizing an adenosine-mediated anti-oxidative pathway ppt

Báo cáo khoa học

... the cAMP-mediated anti -oxidative pathway and leads to increased vulnerability to oxidative damage The SE effect is critically dependent on amino acids Q/R70, K/R71, and A74 of the DR-β chain, the ... membrane suspension (10 μg of proteins) to the incubation medium The total reaction volume was 70 μl Incubations were performed for minutes at 32°C To stop the reaction, μl of 0.5 M EDTA (pH 8.0) ... one hand and the proposed role of oxidative stress in the pathogenesis of RA [10-15] on the other, we proceeded to examine whether the SE could affect ROS production To that end, we first studied...
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Báo cáo khoa học:

Báo cáo khoa học: " Correlating metabolic and anatomic responses of primary lung cancers to radiotherapy by combined F-18 FDG PET-CT imaging" ppsx

Báo cáo khoa học

... primary factor for variations in SUV after treatment was the reduction of metabolism due to cellular death or less likely, in case of an effective treatment, augmentation of metabolism due to tumor ... changes of size versus SUV with the axes cross the metabolic response line of -25% and anatomical response The percentage changes of size versus SUV with the axes cross the metabolic response line of ... Page of (page number not for citation purposes) Radiation Oncology 2007, 2:18 tion of the metabolism to the required response level (left upper quadrant of Fig 1) This patient was found later to...
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