... and Guodong Chen Characterization of Impurities
and Degradants Using Mass Spectrometry
Mike S. Lee and Mingshe Zhu MassSpectrometryinDrug Metabolism and Disposition:
Basic Principles and ... physiological
substrates. Depending on the remaining structure, the formed radical products
of drugs can yield quinones, quinone imines, diimines, dimers (and other
polymeric products), and hydroxylamines and/ or nitroso ... integrated into an inseparable arena
in drugdiscoveryand development as well in related academic research
activities. This book provides a unique thesis on massspectrometryin drug
metabolism...
... L., and McLuckey, S. A. (1988) MassSpectrometry /Mass
Spectrometry Technzques and Applications of Tandem MassSpectrometry VCH,
New York.
16. Hogeland, K. E , Jr and Demzer, M L (1994) Mass ... peptides and proteins. However, only a few of the present generation of
scientists involved m MS of peptldes and proteins know that MS of peptides
was already an active field 30 years ago and ... sample (17), and run another PSD
spectrum.
The Identification
of Electrophoretically Separated Proteins
by PeptideMass Fingerprinting
John S. Cottrell and Chris W. Sutton
1. Introduction...
... meeting criteria
for inclusion and not meeting any exclusion criteria were
invited to participate, and were included after acce pting
and signing an informed consent form.
Methods
Visits in both ... Researchers in the experimental group received train-
ing in the PRECEDE model before patients were
included in the study. This specific training involved
two steps: first they were instructed ... PRECEDE (Predisposing, Reinforcing, Enabling, Causes in Educational Diagnosis, and Evaluation) education model in
the metabolic control and the reduction of cardiovascular risk factors, in patients...
... gel during
staining and destain-
ing. Increase the
staining/destaining
time
Restain the gel. Reduce
the destaining time or
use a dye that stains
proteins indelibly, e.g.,
Procion navy ... always be maintained in the cold
and stored in lysis buffer. Proteins may also be carbamylated by isocy-
anate impurities in the urea solution, and therefore it is worth investing
in the highest ... buffer.
6. Fix and stain as in Section 3.3., step 4, again adding l-2 mg of dithio-
threitol to the fixer/destainer, Coomassie brilliant blue, and dH20 used
to rinse the peptide- containing PVDF membrane....