mononucleosomes dinucleosomes and nucleosome arrays in vitro and analysis of transcription factor bind
Báo cáo y học: "Computational identification of developmental enhancers: conservation and function of transcription factor binding-site clusters in Drosophila melanogaster and Drosophila pseudoobscura" ppsx
... Predicted and aligned binding sites in pCRMs Predicted and aligned binding sites in pCRMs Predicted binding sites and aligned binding sites (see Materials and methods) in positive, ambiguous and negative ... pCRMs: the number of aligned binding sites; the density of aligned binding sites; the number of aligned plus preserved binding sites; and the density of aligned plus preserved binding sites All values ... ortholog The location of binding sites in D melanogaster, binding sites in D pseudoobscura and aligned binding sites along with the average density of sites (700-bp windows) are shown in the bottom three...
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algorithms for representation and discovery of transcription factor binding sites
... performance of all basic methods in discriminating between binding sites for the transcription factor of interest and binding sites of other transcription factors; however, weighting each match ... completely inside one another in the genome In these cases, when scoring the negative binding site, a true binding site for the transcription factor of interest is present; thus these corresponding binding ... 2.2.2 Approaches for representing and searching for transcription factor binding sites Four basic approaches for representing and searching for transcription factor binding sites were evaluated We...
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Báo cáo y học: "Explicit equilibrium modeling of transcription-factor binding and gene regulation" pdf
... free energy of binding for all possible bases at each position in a binding site [9] The algorithm then integrates over the affinities of all possible binding sites within a region of interest by ... Representation of binding specificity and the prediction of binding affinity Pi = [ X] K d,X,i + [X] where Kd,X,i is the predicted equilibrium dissociation constant for X binding to window i and [X] ... consists of windows i and j; it is analogous to Ka,eff,X,i, the equilibrium constant for monomeric X binding to site i in that it is the product of an intrinsic binding affinity, Ka,XY,i,j, and a...
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báo cáo hóa học:" The effect of abductor muscle and anterior-posterior hip contact load simulation on the in-vitro primary stability of a cementless hip stem" ppt
... 5(3):283-93 37 Cristofolini L, Vinteconti M: Towards the standardization of in vitro load transfer investigations of hip prostheses Journal of Strain Analysis for Engineering Design 1999, 34(1):1-15 ... Measurement of the Relative Motion Between an Implant and Bone under Cyclic Loading Strain 2004, 40(1):193-202 43 Monti L, Cristofolini L, Toni A, Ceroni RG: In vitro testing of the primary stability of ... Hip joint loading during walking and running, measured in two patients J Biomech 1993, 26(8):969-90 53 Taylor ME, Tanner KE, Freeman MA, Yettram AL: Stress and strain distribution within the intact...
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Báo cáo vật lý: "Evaluation of In Vitro Antioxidant Activity of 5H-dibenz[b,f]azepine and Its Analogues" ppt
... absent of the N–H absorption band at 3400 cm–1 and the presence of the C=O stretching band at 1600 cm–1, whereas in compounds (a) and (d), the presence of N–H stretching and the absence of C=O ... peroxidation was induced by adding 10 µl of FeCl3 (400 mM) and 10 µl of L-ascorbic acid (200 mM) After incubation at 37oC for hr, the reaction was terminated by adding ml of 0.25 N HCl containing 150 ... presence of NaNH2 as a strong base in the reflux condition, and compound (f) was obtained by using acid chloride, i.e., acetyl chloride, at RT instead of using acetic anhydride in the presence of NaNH2,...
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Báo cáo lâm nghiệp: "In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker" ppsx
... proliferating the axillary shoot within 2– weeks of culture Out of the various cytokinins tested, BAP was the most effective for inducing bud break and growth in axillary meristems Kinetin alone or in ... shoot having 3–4 nodes) within weeks of subculture (Tab II) (Fig 1b) Analysis of variance for the effects of cytokinins and auxins on production of multiple shoots and average number of shoots/explant ... with 0, 0.25, 0.5, 1.0 and 1.5 mg/L of IAA, IBA and NAA alone or in combination for induction of rooting In case of rooting experiment, 10 replicates per treatment were taken and the experiment was...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant adenoviral ... the indicated time-points For this, to 10 pellets per group and time point for each donor were pooled and homogenized using a pellet pestle and repeated titration in mL of Trizol reagent (Invitrogen, ... analyses, sections were washed for 20 minutes in Tris-buffered saline (TBS), and incubated in 5% BSA (Sigma, St Louis, MO, USA) Following washing in TBS, Page of 15 (page number not for citation...
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Báo cáo y học: "The triple combination of tenofovir, emtricitabine and efavirenz shows synergistic anti-HIV-1 activity in vitro: a mechanism of action study" pdf
... Olsen DB: Inhibition of HIV-1 reverse transcriptase by a quinazolinone and comparison with inhibition by pyridinones Differences in the rates of inhibitor binding and in synergistic inhibition ... fitting of the data using the single ligand binding equation y = (Bmax × [ligand])/(Kd + [ligand]) The DEC formation by TFV-terminated DNA/RT in the presence of dCTP yielded Bmax = 49.2 ± 2.7% and ... polymerase- and RNase H-binding orientations, where the RNase-H/non-polymerase binding orientation was favored [57] This nevirapine-based complex therefore showed a distinct pattern of binding orientations...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and ... decorin and fibromodulin with transforming growth factor beta Biochem J :527534 24 Iozzo RV 1999;The biology of the small leucine-rich proteoglycans Functional network of interactive proteins ... JE, Santoro SA 1995;The spatial and temporal expression of the alpha beta integrin and its ligands, collagen I, collagen IV, and laminin, suggest important roles in mouse mammary morphogenesis...
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Review and analysis of renewable energy perspectives in Serbia
... 2009 [17] Ministry of Energy and Mining Renewable Energy in Serbia Ministry of Energy and Mining, 2009 [18] Statistical Office of the Republic of Serbia Energy Balance of Electricity and Heat, ... Republic of Serbia Strategy of development of energy industry of Republic of Serbia until 2015, draft report, 2005 [6] EPS – Electric Power Industry of Serbia, Ministry of Energy and Mining of the ... involved in a number of research projects as a project manager/ senior energy expert in the fields of energy policy and modelling, development & administration of DSS and in lectures and seminars...
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University buildings in Greece: Energy analysis of heating and cooling demand
... because of the increased internal gains resulting from the presence of significantly higher number of people in the spaces The comparison of cooling energy demand profiles of classroom (Figure 5) and ... Society of Heating, Refrigerating and Air-Conditioning Engineers, Inc., Atlanta, USA, 2005 ASHRAE Handbook HVAC Applications American Society of Heating, Refrigerating and AirConditioning Engineers, ... simulation of buildings and energy analysis of the electromechanical systems She has participated in a number of research projects during the last years, including “Heating and cooling loads estimation...
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Đo kiểm đánh giá can nhiễu mạng truyền hình cáp The Measurement and Analysis of the effect of interference in Television Cable network
... Video, Voice and Data communications – The Morgan Kaufmann Serries in Networking Editor – 2nd Edition 17 ROHDE & SCHWARZ - SOUND and TV BROADCASTING – CCIR and FFC tv standards – Printed in the federal ... Broadcasting (DVB); Second generation framing structure, channel coding and modulation systems for Broadcasting, Interactive Services, News Gathering and other broadband satellite applications (DVB – ... Video Broadcasting (DVB); Framing structure, channel coding and modulation for cable systems – European Broadcasting Union 13 ETSI EN 302 307 V1.2.1 (2009-08) – Digital Video Broadcasting (DVB);...
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Tài liệu Psychometric properties of the quality of life scale Child Health and Illness Profile-Child Edition in a combined analysis of five atomoxetine trials pdf
... almost no ceiling and floor effects were observed A factor analysis of the sub-domains yielded a 12 -factor solution The domainlevel factor analysis identified six factors, the four domains of Satisfaction, ... data from clinical trials studying atomoxetine and using the CHIP-CE in the Lilly data base were included The total number of patients included in the combined analysis was 794 Three of these studies ... whole and for the domains and sub-domains All visits and all five studies were pooled for this analysis Inclusion of patients receiving active treatment and placebo in the analysis over time will increase...
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Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf
... identied in an alanine scanning mutagenesis study of FVIIa, shedding light on the amino acid residues that are important for TF binding and or the cofactor effect [13] In terms of intramolecular ... association and dissociation phases were and 10 min, respectively To assess the effect of active site inhibitor incorporation on sTF binding kinetics, native and fFR-cmk-inhibited G372A-FVIIa and FVIIa ... Identication of regions of bovine factor VII essential for binding to tissue factor J Biol Chem 269, 1889118898 19 Persson E, Bak H & Olsen OH (2001) Substitution of valine for leucine 305 in factor...
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Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
... containing the last 326 amino acids in the case of Pta-F1, the last 352 amino acids in the case of Pta-F2, and the last 470 amino acids in the case of Pta-F3 (Fig 2) PCR reactions performed in ... C-terminal end The members of the P-loop NTPase domain superfamily (N-terminal domain in E coli Pta; Fig 2) are characterized by a conserved nucleotide phosphate-binding motif, and are involved in ... Results Expression and purification of E coli Pta and truncated Ptas containing the C-terminal end By analysis of the protein domain architecture of E coli Pta, three conserved domains can be detected...
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Báo cáo khoa học: Insulin induces heme oxygenase-1 through the phosphatidylinositol 3-kinase/Akt pathway and the Nrf2 transcription factor in renal cells pptx
... oxidative insult Insulin is a polypeptide hormone that regulates glucose, lipid and protein metabolism and promotes cell growth and differentiation On ligand binding, the insulin receptor tyrosine kinase ... with increasing concentrations of human insulin resulted in a four-fold induction of HO-1 after h (Fig 1A) Maximal induction of HO-1 protein was achieved at concentrations of 200 nm insulin A ... treated in a similar manner with insulin (200 nm) for increasing periods of time (Fig 1E) A robust induction of HO-1 protein was observed Insulin-mediated induction of HO-1 is PI3K dependent In our...
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Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc
... presence of CMP and AMP (from FAD) GMP was not present in any Qor extract, indicating that the host strains did not incorporate Mo-MGD, or free GMP, into the cofactor binding domain of the Qor protein ... Qor activity and protein content, and determination of the apparent Km and kcat values for quinoline The activity of Qor was determined spectrophotometrically by measuring the quinoline-dependent ... hydroxylase in heterologous hosts, the iorAB genes of Brevundimonas diminuta 7, coding for isoquinoline 1-oxidoreductase, were cloned in P putida KT2440 and in the quinoline degrading strain, P putida...
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Use of Alternative Fuels in Cement Manufacture: Analysis of Fuel Characteristics and Feasibility for Use in the Chinese Cement Sector pdf
... used in cement kilns In comparison to dedicated waste to energy incineration, burning plastics in cement kilns eliminates the challenge of disposing of incineration ash since it can be incorporated ... improving energy efficiency including increasing the share of dry process kilns, consolidating the industry and eliminating the most inefficient producers, and recovering waste heat for electricity ... necessary next step in assessing the viability, costs and benefits of using ASR in cement kilns in China b Textiles According to Chinese government statistics, the textile industry in China produces...
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Báo cáo khoa học: Synergistic co-operation of signal transducer and activator of transcription 5B with activator protein 1 in angiotensin II-induced angiotensinogen gene activation in vascular smooth muscle cells potx
... of STAT5B binding and of c-Jun binding to DNA was detected, respectively, by electrophoretic mobility shift assays (EMSAs) using radiolabeled oligonucleotides containing either a STAT5B-binding ... activity of STAT5B and c-Jun increases in Ang II-induced AGT expression To find out whether the increase in STAT5B and c-Jun phosphorylation induced by Ang II affects the binding of STAT5B and c-Jun to ... specific inhibitor of the JAK-STAT pathway) for 16 h [24], indicating that the activation of STAT5B and c-Jun may be involved in Ang II-induced AGT mRNA expression (Fig 1C) DNA-binding activity of...
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báo cáo hóa học:" Cross-cultural validation and analysis of responsiveness of the QUALIOST®: QUAlity of Life questionnaire In OSTeoporosis" pptx
... statistical analysis and revising the document critically for important intellectual content KS was involved in the drafting of the document RP was involved in the drafting of the document and revising ... 0.1341 Baseline scores based on number of fractures occurring between baseline and endpoint cross-sectional data and 1288 in the analysis of longitudinal data on the psychometric properties of the ... between baseline and endpoint ES = (mean at endpoint – mean at baseline)/standard deviation of change Internal consistency reliability of the QUALIOST® (see Table 1) and distribution of baseline scores...
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- modeling and analysis of skidding and slipping in wheeled mobile robots control design perspective
- design and analysis of migration in parallel evolutionary algorithms
- recent advances in the exploratory analysis of interregional flows in space and time
- karyotype and fluorescent in situ hybridization analysis of human embryonic stem cell and induced pluripotent stem cell lines
- the chitosan as dietary fiber an in vitro comparative study of interactions with drug and nutri
- fret in analysis of transcription complex structure and function
- in vitro toxicological studies of their biotransformation and potential negative effects
- on use of covariates in randomization and analysis of clinical trials
- dose response and dose effect curves to assess the in vitro toxicity optimization of the experiments
- an in vitro comparative study of interactions with drug and nutritional substances
- avidity specificity and sensitivity of transcription factor dna binding in genome scale experiments
- expression function and regulation of transcription factor mef2 in neurons
- in vitro toxicological assessment of heavy metals and intracellular mechanisms of toxicity
- cc patel rm dakhara sl jariwala jk 2010 quot in vitro cytotoxicity study of agave americana strychnos nuxvomica and areca catechu extracts using mcf 7 cell line quot j adv pharm technol res 1 2 pp 245 52
- in vivo and in vitro diagnostic tests of allergy
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