mapping colocation and expression studies of the maize nced and zep gene families
báo cáo khoa học: " QTLs and candidate genes for desiccation and abscisic acid content in maize kernels" docx
... the number of NCED and ZEP genes in the maize genome, to map the genes on the LHRF mapping panel and to determine their expression patterns in the kernel of both maize parental inbred lines The ... analyzing accurately NCED and ZEP expression was the design of specific primers mainly because of insufficient knowledge of the actual number and sequence of NCED and ZEP genes in maize Therefore, we ... maize ZEP1 and ZEP2 genes were closer to each other than to the rice ZEP gene, suggesting a maize specific duplication (data not shown) Mapping of maize NCED genes led to the identification of...
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Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx
... structure and expression analysis of the Cc RNase ment with the terminal sequences of the longer transcript was amplified by PCR and the RNase j gene structure was determined The C capitata RNase j gene ... mRNA isoforms The rectangles represent the exons and the horizontal bold lines the introns The black parts of the rectangles show the protein-coding region, the white parts represent the non-coding ... (SpuUn_WGA56223_2:75333–76952) and Nematostella vectensis (NW_001834409:c1450413–1449631) The numbers above the boxes indicate the size of the exons, and those below the lines indicate the size of the introns (B)...
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Báo cáo khoa học: Expression studies of the core+1 protein of the hepatitis C virus 1a in mammalian cells pot
... levels in the presence of core was the result of an overloading of the cellular protein-synthesis machinery and of a shortage of its components Finally, we examined the possible effect of core+1 ... effect of the core protein on the translation and ⁄ or the stability of the core+1 protein However, no effect of the core+1 protein on core expression could be detected Expression of the core+1 ... downregulates expression of the core+1 ORF [21] In light of these observations, we sought to investigate expression of the core+1 ⁄ S protein under conditions that prevent both core expression and the...
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Expression studies of the proteolytic p10 fragment of the neuronal cdk5 activator in mammalian cells
... result of their concern of having lost something So they wander about and aimlessly search through drawers and closets Some patients rise anxiety for fear of losing track of their caregivers So they ... in the cytotoxicity in AD brains On the other hand, the expression of p39 is similar to p35 in 18 adult brains Moreover, the proteolytic pathway and cleavage of p39 is also similar to that of ... identification of the canonical aging changes and further research on why and how they happen may be a promising way to understanding the phenomena of aging And we may have another way to achieve...
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Expression studies of the proteolytic p10 fragment of the neuronal cdk5 activator in mammalian cells
... result of their concern of having lost something So they wander about and aimlessly search through drawers and closets Some patients rise anxiety for fear of losing track of their caregivers So they ... in the cytotoxicity in AD brains On the other hand, the expression of p39 is similar to p35 in 18 adult brains Moreover, the proteolytic pathway and cleavage of p39 is also similar to that of ... identification of the canonical aging changes and further research on why and how they happen may be a promising way to understanding the phenomena of aging And we may have another way to achieve...
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báo cáo khoa học: "Structure and expression of the maize (Zea mays L.) SUN-domain protein gene family: evidence for the existence of two divergent classes of SUN proteins in plants" pot
... within the maize SUN genes showed that the gene structures are conserved within each class The CCSD genes had two or three exons, and the SUN domain was split between the exons On the other hand, the ... SUN-domain protein genes is provided in Table and the properties and motifs of the CCSD and PM3 subfamilies of these proteins are summarized in Table Conservation of Two Classes of SUN-domain Proteins ... mRNA Expression Profiling of ZmSUN Protein Genes The conservation of the SUN-domain protein genes in plants suggests that they potentially have functions Page of 22 similar to those of their...
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báo cáo khoa học: " Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha" ppt
... on the geometric mean of the expression values of the set of the control genes tested The lower the M value, the more stably expressed the gene is Also, the program enables the exclusion of the ... within the chosen set of samples Depending on the expression level of the genes tested, it is suitable to choose a reference gene with similar expression levels of the tested genes Gene expression ... value of all the other genes and also a change in the M values of the unstable genes, BbrizGDP and BbrizUBI (Figure 3b) To check if the Figure Box-whisker showing the Ct variation of each candidate...
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Báo cáo y học: "Characterization of N200 and P300: Selected Studies of the Event-Related Potential Salil H. Patel 1 and Pierre N. Azzam 2"
... interspersed by a stimulus which deviated from the standard in the intensity of the first component and the glide direction of the second constituent The number of distinct MMN components elicited by ... et al The relationship of age, gender, handedness, and sidedness to the size of the corpus callosum Academic Radiology 1994; 1:243-248 69 Driesen NR, Raz N The influence of sex, age, and handedness ... pathophysiology of the condition [93] Genetic linkage studies involving families with a history of alcoholism show involvement of chromosomes and and possibly chromosome 13, with genetic coding...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... whereas the side chain of Lys173 interacts with both O6 and N7 (Fig 1C) The 2¢OH of the ribose is hydrogen bonded to the main chain and the side chain of Asp142 (Fig 1C) Both hydroxyl groups of the ... II The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The ... Asp142, 2¢ and 3¢OH of the ribose and three water molecules with ˚ coordination distances of approximately 2.4 A The phosphate of the GMP is hydrogen bonded to main chain and side chain atoms of amino...
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Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx
... by the cleavage of the bond between 1¢ and 2¢ carbons of the substrate The epimerization product is generated by the re-formation of the C–C bond after the reorientation of GA, which exposes the ... addition of the enzyme, and the middle three spectra were obtained 18, 35, and 70 after the addition of the enzyme The top spectrum is that of DHMP for comparison Only the NMR signals of the 2¢ and 3¢ ... to the sizes of the Fig Stopped-flow analysis of the binding of HPO to SaDHNA The concentration of SaDHNA was lM, and the concentrations of HPO were 10, 20, 30, and 40 lM for traces 1, 2, 3, and...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... that the work of Lookene et al [27] has already established Of two possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic ... for the WW mutant (Ala substituted for Trp393 and Trp394) and the WWW mutant (Ala substituted for Trp390, Trp393 and Trp394) (C,D) Protein expression of the wild type, the WW mutant, and the ... the C-terminal domain of apoCII interacts with the interface of the N- and C-terminal domains of LPL and part of the lid surface [32] Because the functional importance of LPL’s C-terminal domain...
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Tài liệu Euphorion Being Studies of the Antique and the Mediaeval in the Renaissance - Vol. II pot
... the sunshine of the evening, the noise of the streets, the looks of the crowd, the great minster rising half-finished in the midst of the town by the Rhine, the cries and noise and chipping of ... Roland and the picture of the dying Renaud, I would fain bring before you before speaking of the Euphorion, by Vernon Lee 15 other Roland and the other Renaud, the Orlando of Ariosto and the ... piles of cabbage and cauli-flowers, the rows of tin ware and copper saucepans, the heaps of maccaroni and pastes, of spices and drugs; the garlands of onions and red peppers and piles of apples; the...
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Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf
... parameters The adequacy of the analyses was determined from the reduced weighted sum of squares of residuals, and visual inspection of the distribution of weighted residuals The general expression ... Fluorescence studies of RepA R E M Diederix et al (kex = 295 nm) of free and bound unlabeled C160RepA and wild-type RepA, with the protein concentration constant The ratio of the apparent FW94 of bound and ... global and local A C 5398 dynamics of the AEDANS and W94 probes in free and DNA-bound RepA The decay of the total uorescence intensity was recorded, as well as the decays of its vertically and horizontally...
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Báo cáo khoa học: Cloning and expression of the first nonmammalian interleukin-11 gene in rainbow trout Oncorhynchus mykiss pdf
... from each other by alternate highlighting in bold and italics The ATTTA motifs in the 5¢- and 3¢-UTRs, the start and stop codons, the signal peptide predicted by SIGNALP 3.0 [23] and the potential ... the northern blot (Fig 7), and is the largest of the known IL-11 molecules Interestingly, the trout IL-11 transcript has four potential poly(A) signals (AATAAA), two of them present just 14 and ... upstream of the poly(A) tail in the cDNA sequence, which should be the poly(A) signal giving rise to the 3.2 kb transcript The other two are present before the region that contains the ATTTA motifs and...
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Báo cáo khoa học: Differential expression pattern of the novel serine⁄threonine kinase, STK33, in mice and men doc
... rather reflects synchrony to other events of the spermatogenesis For instance, the mechanisms unleashing the pass of the haploid germ cells through the tight junctions from the adluminal to the ... causing a partial mixing of venal and arterial blood and a decrease of blood flow to the lungs On the other hand, data from the source database [34] suggests a higher expression of STK33 in neuroblastoma ... fitting to the low level expression in tissues other than testis The only discrepancy of the UniGene data, is the fact that we have detected significant Stk33 expression in mouse lung in two of three...
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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
... from the poly(A) of GT-cDNA1 and another is located 11 bp upstream from the poly(A) of GT-cDNA2 (data not shown) Of particular interest is the presence of eight AUUUA motifs in the 3¢-UTR of GT-cDNA2 ... shown) These observations therefore suggest that gcGLUT and human GLUTs1–4 arose by duplication of a common ancestral gene encoding these specific domains In vivo expression and response pattern of ... 2.1 and 3.1 kb in size were detected in the total RNA of grass carp kidney; the larger transcript showed a 30-fold higher expression level than the former (data not shown) Further analysis of the...
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Báo cáo khoa học: Antimicrobial and conformational studies of the active and inactive analogues of the protegrin-1 peptide docx
... at the two spatial tips of the molecule, at the N-terminus with Arg1 and in the turn in the presence of Arg7 and Arg9 (see Figs and 4) The shorter analogue, BM-2, was the most flexible of all the ... of the peptide Two hydrophilic regions were located at the two spatial tips of the molecule, at both termini with Arg1 and Arg17, and in the turn in the presence of Arg8 and Arg10 (see Figs and ... N-terminal parts The conformational differences between the structural families of these peptides applied mainly to the varied structures of the N- and C-termini All the features of the structures...
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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
... comparison of its MS with that of the reference compound The absolute configuration of glycoses was established by capillary GLC of their acetylated (-)-2-butyl glycosides, according to the method of ... stainless steel needle (27 g) was butted against the low dead volume tee and enabled the delivery of the sheath solution to the end of the capillary column The separations were obtained on 90-cm long ... amount of 2-amino-2deoxy-D-galactose was identified in the hydrolysis products of both carboxyl-reduced CPS and LPS [19], confirming the presence of GalNAcA in the native CPS and LPS This was further...
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Báo cáo Y học: Engineering and mechanistic studies of the Arabidopsis FAE1 b-ketoacyl-CoA synthase, FAE1 KCS pot
... other minor protein bands were present, indicating the sample was highly enriched for the FAE1 KCS The expression and accumulation of FAE1 KCS was very low in these samples as evidenced by the ... substitution of basic histidine for the asparagine resulted in inactive enzyme Taken together, the analysis of the decarboxylation activity and characterization of the mutants of the putative ... mutational studies of chalcone synthase have demonstrated that decarboxylation of malonyl-CoA is independent of acylation of the active site cysteine [24] In these studies, substitution of the active-site...
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Báo cáo khoa học: Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. docx
... pattern of expression of a gene [34–39] To evaluate the relevance of the leader intron in cardosin expression, we deleted it from the 5¢-flanking region of the genes (Fig 8) The deletion of the cardosin ... region of the cardosin B gene (from ) 147 bp to + 238 bp) .The 529 bp of the promoter region of the cardosin A gene that is relevant for gene expression in Arabidopsis and the corresponding region of ... of cardosin A, B and D genomic clones with the respective cDNAs (Fig 2) revealed the presence of an intron in the 5¢-UTR of the genes The nucleotide sequences of the cDNA and genomic clones of...
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