... three-dimensional structures of certain domains of nitric oxide synthase isoforms are known [53±57], we are not aware of the three-dimensional structure of the calmodulin binding sites of any isoform ... preventing the formation of nitric oxide (IC50 values at lM concentrations) [49,50] The possibility that the mechanism of nNOS inhibition by the amphibian peptides does involve complex formation of peptides ... inhibition Inhibition of nNOS was measured by monitoring the conversion of [3H]arginine to [3H]citrulline In brief, this involved incubation of a homogenate of rat cerebella (which had endogenous...
... invariant under both translation and dilation Let rk,D (N) denote the maximum possible size of a subset of [1, N] ∩ Z that does not contain any k-term D-progressions Theorem Fix positive integers ... observations His first observation was that Behrend’s use of the pigeonhole principle could be replaced with a number-theoretic result on the number of representations of a huge number as a sum of a ... chosen uniformly from Box0 ) a set with many elements and few types of D-progressions After removing one element from A for each type of progression it contains, we will be left with a set that has...
... selectional restrictions, in the formof DOLCE ontology (Gangemi et al., 2002) classes, on their entities In NLP, information extraction (IE) techniques, for discovering part-whole relations from ... on IE systems that learn instances of these relations from text We have shown that learning from specialized seeds-sets, denoting specific types of the partwhole relations, results in precision ... discovery of part-whole relations Computational Linguistics, 32(1):83–135 M.A Hearst 1992 Automatic acquisition of hyponyms from large text corpora In Proceedings of the 14th conference on Computational...
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... substitutions are clustered in the NH2-terminal half of Tax (20/26) Consistent with the notion that the amino acid substitutions had occurred in important regions of Tax, we noticed that the T130I ... in tax that abrogated induction/stabilization of p21CIP1/WAF1and p27KIP 1also disabled APC/C activation, are in agreement with that conclusion Activation of p21CIP1/WAF1mRNA transcription by Tax ... restriction site in the tax coding sequence, was cloned into LV-Tax-SV-Puro via the BamHI (located immediately upstream of the translational initiation codon) and SmaI (downstream of the M47 mutations)...
... concentrations; significant reductions only appeared for K We not know of any other attempt to analyse flooding effects on xylem sap contents Effects of water stress on xylem sap composition have ... highest concentrations of P Only seldom were the effects of flooding statistically significant Significant reductions only appeared for K and Ca in Q palustris and in Q rubra Large variations between ... observed only limited increases in osmotic potential and ion concentrations with decreasing predawn leaf water potential It may be concluded that the stability of xylem sap concentrations, associated...
... Watson RR (ed.) Nutrition and heart disease pp 83-109, Churchill Livingstone, London, 1983 Gilliland SE, Nelson CR, Maxwell C Assimilation of cholesterol by Lactobacillus acidophilus Appl Environ ... bacteria were spread onto BL agar plates to confirm the survival ofbacteria and anaerobically incubated at 37oC for 48 hrs If colonies were formed on the BL media, they were decided as the bacteria ... microbial identification system (MIDI, Inc., USA) Isolationof bacteria: One hundred strains of LAB were isolated from selective (TATAC, LBS and BS) and nonselective (BL) media; 18 Streptococcus...
... FAB classifications, as well as from normal donors Informed consent was obtained from all patients prior to study according to the regulations of the Institutional Review Board of Rush Medical ... Journal of Hematology & Oncology 2008, 1:8 http://www.jhoonline.org/content/1/1/8 administered as a single dose at the start of cell culture, was found to contain a mixture of various conformations ... than thatof the known conventional differentiating agents It may be that the binding of these peptides results in an uncoupling of the expression of the proliferation and differentiation markers...
... consent regarding participation in the study Initial observation has been done on the group of 18 patients with acute coronary syndrome (11 males and females aged from 47 to 68) Once conditions ... Taq polymerase First run of amplification was conducted under cycling conditions consisting of an initial denaturation at 95°C for min, followed by 45 cycles of denaturation at 95°C for 30 sec, ... primer-spanning region of C pneumoniae 16S rRNA gene These standard plasmid dilutions covered the range of plasmid concentration corresponding from to 1,0 copies/µl According to the results obtained from the...
... further investigation of the dynamics of the bacteria in lakes with cyanobacteria is required CONCLUSION We successfully quantified the microcystin-degrading bacteria in a biofilm from a practical ... Provided by Dr Fujimoto from Tokyo University of Agriculture ** Biofilm samples Samples of biofilm (10 g) were taken from a biological contact material, honeycomb catalyst, of a practical biological ... amplification products showed no increase, or a slight increase for the Y2 strain) (Fig 1B) A B Fig - (A) Comparison of detection of mlrA gene using QMF and QMR (B) Comparison of detection of mlrA...
... numbers of CFU obtained after h of incubation with and without the tested protein (five experiments) The number of dead bacteria after h of incubation in buffer alone (control) was £ 2% for all bacteria ... we have not determined its exact mechanism of action, we have shown that the antibacterial ⁄ fungicidal properties of trappin-2 involve the cationic nature of the molecule, as assessed from the ... number of surviving colony forming units (CFUs) of all bacteria tested in a dose-dependent manner, except for K pneumoniae and H influenzae, which were less sensitive at high doses than at low doses...
... standard deviation (SD) or median and range A comparison of the morphometric data obtained from culture of CD and non-CD subjects was done using one way ANOVA All statistical analysis was performed using ... (Figure 3) Discussion Q-banding analysis of FBs from CD and non-CD subjects demonstrated a normal and stable karyotype (data not shown) Morphometrical analysis performed on CD and non-CD FBs images ... [28] Differently from these specimens, intestinal endoscopic biopsies contain a small amount of a soft tissue and have an important bacterial contamination caused by the common intestinal flora,...
... generation virus, but to thatof wild type virus, containing all viral genetic elements, suggesting that transcription and expression of viral genes cannot fully account for the observed reduction of ... suggesting that the immune response may not be the cause of suppression of this key hepatic isoform At later timepoints, CYP mRNA levels were suppressed in animals given HDAd in a manner similar not only ... p ≤ 0.01) Effect of a single dose of virus on hepatic CYP2C11 expression and function CYP2C11 protein expression was significantly suppressed six hours after administration of the WT (37%) and...
... against Salmonella sp The antibacterial activity of LAB may often be due to the production of organic acids, with a consequent reduction in ´ pH, or to the production of hydrogen peroxide (Gonzalez ... intestine of 1341 chicken, the total concentration of bile salts is about 10–11 mmol kg-1 digesta and the proportion of conjugated to unconjugated bile varies according diet However, the conjugated forms ... sulfhydryl groups causing denaturing of a number of enzymes, and from the peroxidation of membrane lipids, thus increasing membrane permeability (Condon 1987; Kong and Davison 1980) LAB strains were reported...
... SD ofthatof a pool of sera from NHS All ELISAs were performed in duplicate To check for possible false-positive results because of the presence of the GST moiety in the α-fodrin–GST product that ... observations underline once more the importance of an easy-to-perform alternative biochemical technique to confirm ELISA data [19] Conclusion Based on the difficulties encountered in this study to confirm ... SjS, contained antiRo60 and contained anti-La, while of the 10 IgG-positive sera, all contained anti-Ro60 also and contained anti-La antibodies (Table 1) Four of six sera from patients with secondary...
... statistics is not due to either a genuine lack of variation in metabolic activity or the poor resolution of this variation Instead, our results imply thatthat quantification of tumor composition via ... regions of lesser intensity, with each type of region being wholly contained within the bounds of the tumor Since both the rate of proliferation [8] and the rate of healthy tissue invasion [7] ... maintain that a shift of focus from tumor composition to shape and location offers immediate potential for improved clinical therapy Consider that the uncertainty in the anatomical placement of brachytherapy...
... terms of cfu/ml for all the samples analyzed and tabulated in Table Figure Demonstration of bacterial isolation process (a) The isolationof aggregates ofbacteria (S aureus) and CCGAMION from ... obvious that with the increase of concentration of bacterial solution, we can easily isolate bacteriafrom the solution Herein, mixing of gum arabic modified Fe3O4 with collagen to bacterial solution ... investigations of nucleation, growth and also of interaction energies of surfactants with thatof the embryos [32] Furthermore, the CCGAMION was separated along with the bacteria using the interactions...