isolation and characterization of embryonic and adult epicardium and epicardium derived cells
Synthesis and characterization of silicon nanowires using tin catalyst for solar cells application
... contact between the SiNWs and neighboring SiNWs Moreover, it might be the effect of Fig (a) Schematic of the SiNWs solar cells FE-SEM images of the (b) as-synthesized SiNWs and (c) phosphorous-diffused ... characteristics of the fabricated SiNWs solar cell under dark and light conditions the thickness of Si base substrate more thinly For improvement of conversion efficiency of the SiNWs solar cells, the ... Therefore, further reduction of the reflectance will be expected from the optimization of the synthesis conditions of SiNWs For analysis of the electrical properties, the I–V curve of the fabricated SiNWs...
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Báo cáo y học: "Early Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response" ppt
... in pathogen recognition and induction and regulation of the innate and adaptive immune response Airway epithelial cells express TLRs and activation of TLRs on epithelial cells has been shown to ... and protein expression and leads to the down-regulation of TLR1, TLR5 and TLR6 expression There is a strong synergism of IFN-γ and TNF-α that results in a highly elevated expression of TLR2 and ... secretion of IP-10, MIP-3α and GRO-α The TLR2 ligand zymosan induced an elevated secretion of GRO-α (Fig 2A) Other TLR2 ligands including Pam3CSK4 and PGN had no significant impact on the secretion of...
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báo cáo khoa học: "Optical characterization of colloidal CdSe quantum dots in endothelial progenitor cells" pptx
... denotes the conduction band edge and VB the valence band edge Referring to the vacuum level as potential energy zero, the CB of CdSe is -4.95 eV (electron affinity of CdSe), the band gap Eg (energy ... Optical characterization of colloidal CdSe quantum dots in endothelial progenitor cells Journal of Nanobiotechnology 2010 8:2 Submit your next manuscript to BioMed Central and take full advantage of: ... emission peaks (616 nm and 613 nm) when 458 nm and 514 nm LASER wavelengths are used for cluster and in Figs 4(a-b) and 6(a-b) The most probable reason is the merging of the laser signal with...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular ... as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the...
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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... positions of the Ca of Ala156 in HYDJs and the Ca of Met156 in HYDBp located in the SGL3, which leads to the increased size of the substrate binding pocket of HYDJs The side chain of Ala156 is ... performed using T-COFFEE [44] Docking simulation of substrate on the active site of HYDJs The substrate binding pockets of HYDJs and the mode of interaction between substrate and enzyme were analyzed ... SGL2 and SGL3, correspond to residues 60–71, 91–99 and 151–161, respectively On the basis of the homology model, the SGLs of HYDJs and HYDBp (PDB accession number 1NFG) were superimposed and compared...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... for cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of oligo(dT) primer and 10 mm of dNTPs were incubated for at 65 °C Subsequently, lL of the first strand buffer X and lL of dithiothreitol ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5.2 for the four isoforms respectively Secondary structure of the pulchellin isoforms ... mixture of other isoforms (B) Gel visualization of proteins eluted Lane 1, molecular weight markers Numbers on the left indicate the Mr values of the standards in thousands Lanes 2–9, lg of peak...
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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
... of 50 nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and ... of 0.6 lM The data were fitted to Michaelis–Menten curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of ... Asterisks and broken line: data and fitting with PHI/PHO ratio of hypothesis and therefore a direct involvement of PHI in electron transfer This conclusion is consistent with the very low degree of identity...
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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt
... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for ... the first time the isolation and biochemical characterization of D desulfuricans ATCC 27774 ccNiR subunits The stoichiometry between NrfH and NrfA is discussed The reassessment of previous spectroscopic...
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Báo cáo khoa học: Identification and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot
... 14693–14698 Van Hoof, C., Ingels, F., Cayla, X., Stevens, I., Merlevede, W & Goris, J (1995) Molecular cloning and developmental regulation of expression of two isoforms of the catalytic subunit of protein ... were derived from prophase oocytes after addition of lM progesterone and overnight incubation of the oocytes at 18 °C Several Xenopus adult tissues (liver, gall bladder, spleen, heart, muscle and ... revealed that a large part of the 5¢-UTR and the complete N-terminal Ó FEBS 2003 specific part of HPR130 reside in one exon of 2435 bp (exon 2a) Comparison of the XN73 and XPR130 sequences with...
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Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt
... 10 lL of a solution of 0.2 M NaCl containing 100 lL of carrier tRNA and 40 lL of ethanol was added to the sample and kept at °C for 60 The mixture was centrifuged (15 000 g for 20 min) and the ... biosynthesis, and still in the range of the IC50 (0.03–4 nM) of type I RIPs The IC90 (the concentration of RIP causing 90% inhibition of translation) of lamjapin in the cell-free system of rabbit ... release more than one mole of adenine from each mole of ribosomes in 10 and even up to 12 mol of adenines in 40 (Fig 4B) Base and position specificity of lamjapin in depurination of SRD RNA Synthetic...
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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc
... Chlamydomonas cells Chlamydomonas CW15 cells were grown to a cell density > ´ 106 cells per mL Cells were pelleted, resuspended in 30 mM Tris/HCl pH 7.9, and broken by two cycles of freeze-thawing ... RESULTS Cloning and sequences of Ch-Prx1 cDNA and the Ch-Prx1 gene In order to complete the sequence data for this new protein, and to be able to make a thorough characterization of its biochemical ... BLAST of peptide sequences of 21 kDa proteins in databases Tryptic peptides were puri®ed by RP-HPLC and some of them were totally or partially analyzed by Edman sequencing (ááá) and/ or by MALDI-TOF...
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Báo cáo khoa học: Characterization of rat cathepsin E and mutants with changed active-site residues and lacking propeptides and N-glycosylation, expressed in human embryonic kidney 293T cells ppt
... of the formation of intracellular organelles and without alteration of the morphology of vacuolar compartments, resulting in the profound inhibition of the endosomal ⁄ lysosomal degradation of ... HEK-293T cells In this study, we used HEK-293T cells to express wildtype cathepsin E and its mutants and to follow the processing, maturation, and trafficking of these proteins, as these cells have ... propeptides, and N-glycosylation of cathepsin E in its processing, maturation, and trafficking must be of particular importance In this study, to understand the molecular basis of the processing and intracellular...
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Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx
... mouse- and human -derived membrane preparations were examined for PEN-2 immunoreactivity and revealed strong detection of a 10-kDa band in PS1+/ +neural stem cells, embryonic brain, and SY5Y cells; ... full-length PS1, NTF, CTF and PEN-2 NCT was detected as the mature form APH-1b was detected primarily as a major band of 36 kDa (possible dimer) and as minor bands of 18 kDa and possible trimer at ... regions of APH-1a (short form) and APH1b, and to the loop four region of APH-1b (data not shown) Investigation of noncarbonate washed embryonic mouse brain PS complex revealed similar detection of...
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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx
... presequence of 19 amino acids The mature protein would have a molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and the d-CDes from Arabidopsis showed an overall identity of 36% and ... d-CDes mRNA and protein levels in older plants, during the light phase in a diurnal light ⁄ dark rhythm and under sulfate limitation Results In silico characterization and isolation of the Arabidopsis ... protein has a predicted molecular mass of 43.9 kDa and a pI of 7.2 It contains relatively high amounts of the sulfur amino acids cysteine (four residues) and methionine (10 residues) According...
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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx
... Import and assembly of proteins into mitochondria of mammalian cells Biochim Biophys Acta 1592, 97–105 19 Suzuki, H., Maeda, M & Mihara, K (2002) Characterization of rat TOM70 as a receptor of the ... ampicillin and/ or 2% (w/v) glucose Solid media contained 2% (w/v) Bacto-agar Transformation of E coli was by standard procedures [24] performed using electrocompetent cells Isolation of total RNA ... possibility of inter- and intra CDR disulphide cross-links These strategies are summarized in Fig 1B and Table 1, and details of the library construction are given in greater detail in the Materials and...
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Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx
... strain genuinely reflects of the sensitivity of the mutant toxin to temperature To obtain a clearer picture of the growth profiles of yeast cells expressing these RTAs, cells were plated at various ... permissive temperatures of 30 °C and 37 °C, respectively A sample of the cells was removed from each culture, rRNAs were isolated in TRIzolÒ (Invitrogen, Paisley, Scotland), and the extent to which ... site molecules Y80, Y123 and E177 are shown in green and the position of the two mutated amino acids, F108 and L151, are shown in blue between the aromatic rings of Tyr80 and Tyr123 close to the...
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Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx
... types of plastids The Ndh complex associates in homodimers and dissociates in membrane and soluble subcomplexes; isolation of the Ndh complex by BN / PAGE and Tricine / PAGE Intact MS and BS ... exists as a monomer and dimer and splits into a membrane and soluble subcomplexes Separation of the Ndh complex by CN / PAGE (1D) and Tricine / PAGE (2D) and CN / PAGE (1D) and BN / PAGE (2D) ... GELGIYLVGDDSLFPWR and IRPPGFINLQILPQLVK (one missed cleavage) All of these peptides were part of the maize NdhH subunit Database analysis of a MALDI-TOF-MS measurement of a 21-kDa trypsin-digested gel band...
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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot
... Switzerland), thermolysin, chymotrypsin or elastase (Sigma, St Louis, MO, USA)] and Cloning of the bovine MUC15 cDNA by PCR with degenerate primers Isolation of total RNA from the mammary gland of ... PCR screening of bovine and human cDNA libraries showed the presence of MUC15 mRNA in human breast, bovine mammary gland, bovine lymph nodes and lungs of both species (Table 1) Of the identified ... examined and specific PCR primers were designed enclosing the coding sequence of the bovine protein To investigate the presence of MUC15 expression in epithelial cells of the human mammary gland,...
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Báo cáo khóa học: Isolation and biochemical characterization of two soluble iron(III) reductases from Paracoccus denitrificans docx
... pH 7.4, and washed by the same buffer The proteins were eluted in two phases with 300 mL of linear gradient of 0–0.6 M NaCl and 50 mL of linear gradient of 0.6–1 M NaCl at a flow rate of 0.8 mLÆmin)1 ... calibration of the column with standard proteins of known molecular masses, the molecular masses of native FerA and FerB were estimated as 25 kDa and Table Purification of P denitrificans Fe(III) reductases ... to this enzyme On the other hand, the solution of FerB was yellowish in color and exhibited absorption bands at 380 and 448 nm (Fig 3), diagnostic for the presence of a flavin group When 0.4 mM...
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Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt
... of degenerate and specific primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... upper strands with [32P]ATP[cP] and T4 polynucleotide kinase The labeled oligomers were annealed by heating to 90 °C an equimolar mixture of the upper and lower strands in reaction buffer and cooling ... -2 and -3) The Xenopus XHIVEPs are characterized with respect to temporal and spatial expression, nuclear import/export activity and DNA binding specificity Materials and methods Isolation and...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25