interpretation of in vitro skin absorption studies of lipophilic chemicals
Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding ... altered protein–protein interactions, which may explain why the Chlamydomonas loop region is exposed to trypsin in the in vitro assay The in vitro import experiments suggested that the loop of Arabidopsis ... shown in Fig 4C Interestingly, both tags are recognized more efficiently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G in vivo In order to examine the topology of...
- 9
- 422
- 0
Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... (Fig 3E) Interestingly, the inclusion of IKK2 inhibition by SC-514 with our experimentally determined rate constants in the original model resulted in a delay in simulated peak and damping of subsequent ... simulated in silico Combining this method of analysis (in vitro, cell-based and in silico analysis) will facilitate systematic understanding of the underlying properties of this signaling pathway ... = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software measures the intensity of NF-jB in...
- 13
- 475
- 0
Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
... determined following the hydrolysis of the carboxyl-reduced CPS Presence of L-alanine was confirmed by amino acid analysis For this, 0.6 mg of CPS was hydrolysed in 5.7 M hydrochloric acid containing ... TOF Analysis of the in vivo cultured A salmonicida strain 80204-1 cells In vivo culture was performed using ligated dialysis tubing bags filled with bacterial suspension of A salmonicida strain ... The extent of amidation could be determined by the intensity ratio of fragments at m/z 662.2 Da (GalNAcAN-containing trisaccharide repeating unit) and m/z 663.2 Da (GalNAcAcontaining trisaccharide...
- 10
- 332
- 0
báo cáo hóa học:" Functional significance of the signal transduction pathways Akt and Erk in ovarian follicles: in vitro and in vivo studies in cattle and sheep" ppt
... Effects of active immunization of sheep against an amino terminal peptide of the inhibin αC subunit on intrafollicular levels of activin A, inhibin A and follistatin Journal of Endocrinology ... important in mediating the effects of FSH on inhibin-A, activin-A, oestradiol and progesterone secretion and also important in mediating IGF-I stimulated inhibin-A, activin-A, follistatin, oestradiol ... resulted in a final follicular fluid concentration of 50 μM of the inhibitors, and 50 μM (0.005%) of the DMSO Concentrations of the inhibitors were based on the treatments used in vitro in Experiment...
- 13
- 438
- 0
Báo cáo y học: "Inhibition by Natural Dietary Substances of Gastrointestinal Absorption of Starch and Sucrose in Rats 2. Subchronic Studies" pptx
... composed of inhibitors of the gastrointestinal absorption of starch and sucrose were effective at lessening their absorption in rats and pigs [26] To determine whether the CHO blocking effects ... seventh and ninth weeks of the subchronic study, the rats receiving the formula containing a variety of natural ingredients reputed to influence CHO absorption were individually Int J Med Sci ... acute studies [26] After weeks of receiving the formula containing carb blockers, it was still able to lessen CHO absorption similar to that seen in the acute studies [26] The utility of combining...
- 7
- 290
- 0
Báo cáo khoa học: "In vitro studies on the modification of low-dose hyper-radiosensitivity in prostate cancer cells by incubation with genistein and estradiol" pot
... use of FCS-containing media during genistein incubation and the incubation with higher genistein doses Mechanisms of interaction To search for mechanisms of interaction we investigated protein ... Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in human esophageal cancer cell lines in vitro: possible involvement of inhibition of survival signal transduction pathways Int ... after incubation with genistein concentrations of 15 – 30 μM in FCS-containing media [41] In this study the NF-κB activity was investigated, too An inhibition of radiationinduced activation of NF-κB...
- 12
- 368
- 0
Báo cáo y học: "Data from necropsy studies and in vitro tissue studies lead to a model for allometric scaling of basal metabolic rate" pot
... tissues of the skin and gastrointestinal tract, the midpoint of the values of bi is 0.71 The midpoint of the values of ki is -0.14, the midpoint of the values of scaling exponents for the SMR of tissues ... values of βi This midpoint is estimated as the midpoint of the values of ki plus the midpoint of the values of bi because values of ki are not available for certain tissues For scaling of the brain ... accounts for nearly all of the mass of skin, is assumed to be close to the estimate of the scaling exponent for skin, 0.96 The scaling exponent for the mass of the gastrointestinal tract is also close...
- 8
- 356
- 0
In vitro and in vivo studies into the antidiabetic and antilipidemic effects of chlorogenic acid
... Activation of insulin signaling upon binding of insulin to insulin receptors causes suppression of gluconeogenesis in liver and kidney [14], translocation of glucose transporter-4 (GLUT 4) from inner ... [24] VI Incretins Incretins are hormones secreted by intestine in response to nutrients ingestion Their main effect is to stimulate pancreas to release insulin after meals intake Two incretin hormones ... predominant insulin resistance with relative insulin deficiency or predominant insulin secretory defect with insulin resistance The etiology of this form of diabetes is wide and complicated, ranging...
- 190
- 586
- 0
Pathobiological studies of zoonotic blastocystis subtypes using in vitro model systems
... disorder of gastrointestinal tract presenting with abdominal pain and altered bowel habits Compared to healthy controls, a higher incidence of Blastocystis in IBS patients is reported in several studies ... Diseases, 15th-16th April 2010, Singapore Haris Mirza, Joshua DW Teo & Kevin SW Tan Role of Rho associated kinase (ROCK) in Blastocystis induced increase in intestinal epithelial permeability The ... syndrome IFN Interferon IgA Immunoglobulin A IL Interleukin IMDM Iscove’s modified Dulbecco’s medium iNOS Inducible nitric oxide synthase Min Minute ml Milliliter MLC Myosin light chain MLCp Phophorylated...
- 275
- 571
- 0
In vivo and in vitro studies of th17 response to specific immunotherapy in house dust mite induced allergic rhinitis patients
... treatment were used in the following in vivo and in vitro studies 3.3 In vitro Study To mimic the functional response of Th17 and other Th cell subpopulations to HDM allergens, an in vitro allergen ... between in vivo and in vitro findings, confirming that not only Th2, but also Th17 cells play an important role in the context of allergic inflammation IL10 producing CD4+ T cells showed a distinct ... markers in each study group tested in vitro 3.2 In vivo Study 3.3.1 Th17 specific gene mRNA/protein expression Following a 3-day in vitro stimulation, there were mRNA/protein levels of Th17 associated...
- 11
- 297
- 0
In vitro studies on the color stability and masking ability of composite cores and the influence of posts and cores on the shades of all ceramic systems
... composite core materials of varying thickness Table 4.2 The order of preference of composite cores in masking the posts 57 60 viii SUMMARY The aims of these in- vitro studies are to evaluate the ... significant influence in the masking ability As the thickness of the composite core and the all-ceramic material increased the masking ability of these materials improved All the above mentioned findings ... an increase in scattering within the material Light scattering in a material is the result of scattering centers that cause the incident light to be scattered in all directions The scattering...
- 108
- 461
- 0
The role of caspase 1 in a murine model of influenza pneumonitis, and studies on cell death inhibitors in vitro
... Summaries of experimental procedures 54 2.29.1 Influenza A infection of RAW264.7 murine macrophage cell line in vitro 54 2.29.2 An investigation of the role of caspase-1 in an in vivo ... Expression of caspase-1 and growth of virus in influenza A/PR/8/34 (H1N1) infection of RAW264.7 macrophages 130 4.3 Insights into the role of caspase-1 using a murine model of influenza ... foetal bovine serum g gram h hours xv H&E haematoxylin and eosin stain HRP horseradish peroxidase ICE interleukin-1β-converting enzyme IFN interferon IL interleukin INF infected IRF interferon-regulatory...
- 190
- 824
- 0
Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
... the loading of pravastatin into erythrocytes was directly proportional with increase of drug concentration in the incubation medium, in the 2-10 mg/ml concentration range This finding is in concurrence ... pravastatin However, the relative impacts of different in vitro findings on the overall in vivo drug delivery efficacy of these cellular carriers remain to be evaluated during the future in vivo studies ... statins including inhibition of the metabolism [5], administration of statins with certain juices [6] or inhibition of Pgp [3] Unfortunately these strategies are frequently associated with increase...
- 9
- 829
- 0
Báo cáo y học: " Preparation of RGD-modified Long Circulating Liposome Loading Matrine, and its in vitro Anti-cancer Effects"
... phosphate-buffered saline (PBS), and resuspended at × 106 cells /mL in Annexin-V binding buffer The supernatant (100 μL/tube) was incubated with μL of Annexin-V-FITC (Invitrogen, USA) and μL of PI (Invitrogen, ... containing 10% heat-inactivated fetal bovine serum (Nuoding, China), and the HT-29 cells were grown in DMEM medium (GIBCO, USA) also containing 10% heat-inactivated fetal bovine serum All of the ... mini-gel columns of Sephadex-G50 prepared in advance In brief, incubation mixtures of 200μL containing unloaded matrine and RGD-M-LCL were added onto mini-gel columns Then particles of RGD-M-LCL...
- 12
- 635
- 0
Báo cáo y học: "In vitro bactericidal activities of Japanese rice-fluid against Helicobacter pylori strains"
... raw rice Discussion We investigated the in vitro antimicrobial activities of the three kinds of Rice-fluid against various species of microorganisms including H pylori strains by the timekill-curve ... phenomena were observed against other four strains of E coli, five strains of S aureus, S epidermidis, E faecalis and P aeruginosa, and three strains of K pneumoniae and two strains of K oxytoca, respectively ... consisting of three kinds of Rice-fluid derived from the two kinds of raw rice; i e., polished (pH6.32) and unpolished (pH5.98), and the usually cocked rice (pH5.91), and remaining one compound of...
- 5
- 430
- 0
Analysis, sequencing and in vitro expression of PCR products
... functionally analyzed using in vitro assays Furthermore, rapid in vitro protein–protein interaction studies could be performed using this technique where not only full-length version of proteins could be ... also protein fragments for mapping of interaction domains The use of in vitro expression approaches to analyze protein products is likely to gain momentum for the analysis of known proteins as well ... strategies The in vitro expression of proteins can also be used for functional studies of proteins For example, enzymes synthesized in vitro are likely to be correctly folded and to retain enzymatic...
- 24
- 494
- 0
Tài liệu The Centrality of Money, Credit, and Financial Intermediation in Marx’s Crisis Theory: An Interpretation of Marx’s Methodology pptx
... completion of the abstract framework for crisis in SCP requires the integration of the remaining functions of money in the model.22 The incorporation of the function of money as means of payment, ... rate of profit is the ratio of industrial and commercial profits after the deduction of interest and rent - what Marx called profit of enterprise - to invested capital The gross rate of profit ... rates of interest Vigorous capital investment will initially increase profits; in turn, sustained high profits will serve to increase confidence and improve the business climate.48 Assessments of...
- 42
- 874
- 0
Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc
... postinjection), suggesting a high binding capacity of ETA in hepatic parenchyma Indeed, injection of the toxin into mice has been shown to result in an early and profound inhibition of hepatic protein synthesis ... examined the effects of various protease inhibitors on the proteolysis of endosomal ETA and ETA-A, using cell-free endosomes preloaded with ETA toxin in vivo and incubated in vitro at pH in the ... of internalized ETA by cathepsins B and D To confirm the endosomal proteolysis of internalized ETA under conditions that maintained endosome integrity, we used cell-free endosomes containing in...
- 15
- 588
- 0
Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt
... than one binding site for bis-ANS on HSL (Fig 3A) The maximum emission intensity of the complex increased in an inverse hyper- Fig Interaction of bis-ANS with HSL Spectra of bis-ANS obtained at ... phosphorylation This gain in hydrophobic surface area presumably accounts for the increase in in vitro activity of HSL following PKA phosphorylation through increased binding between HSL and the ... phosphorylation induces a conformational change that increases the accessible hydrophobic surface area, enabling freer access to the lipid-binding site, in return for weaker binding This is well in line...
- 11
- 562
- 0
Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
... basis of the structure of the 5¢UTR of the plus-RNA, the structure of the 3¢-end of the minusstrand RNA being at that time unavailable In the present study we investigated the involvement of sequences ... and replication of 3¢-end of HCV minus RNA T Astier-Gin et al Fig Secondary structure of the 3¢ terminal sequences of HCV minus-strand RNA Model structures of domain I (A) and domain II (B, C and ... al Binding and replication of 3¢-end of HCV minus RNA Fig Secondary structure of RNA mutated in the SL-A1 and SL-B1 domains Only the secondary structure of the 151 nt from the 3¢-end of the minus-strand...
- 15
- 597
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25