integrins and vascular development in differentiated embryonic stem cells in vitro pdf
The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain stem cells ... experiments involving blastocyst injections and ectopic grafting in adult hosts cannot be performed in the human Animal embryonic stem cells Embryonic stem cells were first derived from certain strains ... L-glutamine, 1X penicillin–streptomycin, 1X nonessential amino acids (Invitrogen), 1X insulin-transferrin-selenium G supplement (Invitrogen) and mM β-mercaptoethanol (Invitrogen) For the derivation and...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs), and these vascular ... of transplanted vascular cells derived from human ES cells and the vascular density in the infarct area after the transplantation In the saline- and hMNCs-injected groups, the vascular density ... Translational Medicine 2008, 6:54 cells, the number (cells/ mm2/mouse) of single stranded DNA (ss-DNA)+ cells in one field in the ischemic core from each mouse in the saline- or hES-ECs+MCs-injected group...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... reported in human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs ... was repressed in hES cells and upregulated in EB and adult cells NFIB is a transcription factor involved in brain development [7578], chondrocytic differentiation [79] and lung development [78] ... the samples into three groups: hES cells, EB, and adult cells Node A contained the genes that were upregulated in both hES cells and EB, node B contained the genes upregulated in hES cells only,...
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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt
... established Feeder-independent murine embryonic stem cells (ESCs) were kindly provided by K Pfeffer (Institute for Microbiology, Heinrich Heine University of Düsseldorf, Germany) The cells were derived ... were fixed in formalin (4%) until further procession Formaline-fixed microspheres were dehydrated in increasing ethanol concentrations (50%, 75%, 90% and 100%) and embedded in paraffin (Paraplast ... cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure 1) Generally, the mineralization...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... Problems in Medicine and Biomedical and Behavioral Research: Defining death: a report on the medical, legal and ethical issues in the determination of death USGPO Washington DC; 1981 Landry DW, ... end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics J ... Research and Prevention Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent with the President's belief in the fundamental value and...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... effective in vitro system to study complex events underlying human nervous system development (Ben-Nun and Benvenisty, 2006; Dvash et al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells ... culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... forebrain, midbrain, hindbrain and the spinal cord This figure was reproduced from Epstein et al., 1999 2.4 SHH and neural development During the initial phase of neural induction, the ectoderm is induced...
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Identification of oct4 and sox2 targets in mouse embryonic stem cells
... connection between the intrinsic transcription factors and extrinsic signaling pathways in mouse embryonic stem cells (mESCs) Stat3 and Oct4 were shown to bind to the promoter of Nanog, and subsequent ... Sox2 DNA interactions, characterized the joint DNA motif, expanded the Oct4 and Sox2 transcription binding network to the Smad1 and Stat3 signalling networks and utilized these binding data in an ... αOct4 and αSox2 antibodies used in ChIP 3.2 Oct4 and Sox2 binding to Oct4 CR4 region in mESCs 3.3 Oct4 and Sox2 binding reduces after retinoic acid differentiation of mESCs 3.4 Oct4 and Sox2 bind...
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Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery
... Human embryonic stem cells (hESCs) hold great promises in many aspects of research and clinical usage Comparing with other type of stem cells such as adult stem cells and induced pluri-potent stem ... somatic cells They repeatedly introduce certain proteins channeled into the cells via poly-arginine anchors and sufficiently induced pluripotency[45] This new technique brings in new hope in stem ... applications 1.2.2.2 Embryonic stem cells: Another type of stem cells by conventional categorization is embryonic stem cells based on its origin from the fertilized egg In 1981, embryonic stem cells (ESC)...
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Tài liệu Food Security and Sustainable Development in Southern Africa doc
... social development, women and habitat relating directly and indirectly to food security Within the UN system alone, including the international financial institutions, 14 Food Security and Sustainable ... African land policy as implemented in the period 1994 to 1999 Since joining the HSRC, he has been involved in research around integrated rural development including land reform, agricultural development, ... resolving food insecurity and the real achievements in increasing global food security, the gap between the aspiration of eradicating hunger and the continuing reality, portrayed, in this case, in...
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Tài liệu Mathematical and Scientific Development in Early Childhood docx
... starting point for spatial and quantitative thinking remains controversial in the field and used that point to highlight the need for caution in presenting research findings of this kind to the ... mathematics and science learning include categorization, symbolic reasoning, and causal reasoning Unfortunately, these advances in understanding of children’s thinking not seem to be shaping practice and ... first strand, including consideration of financing, minimal licensing standards, and the schedules for care, tend to surround the child care settings serving the poorest families—families leaving...
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Báo cáo khoa học: Transcript profiling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice pdf
... crosstalk between auxin and other plant hormones [21–24] Our study also shows that genes involved in cytokinin (e.g cytokinin-Oglucosyltransferase, cytokinin dehydrogenase, and response regulators), ... Transcript profiling of auxin-responsive genes M Jain and J P Khurana responses, which indicates crosstalk between stress and auxin signaling In the recent past, the identification of F-box proteins (TIR1 ... results reported in this study will be helpful in understanding the transcriptional network regulated by auxin and functional validation of selected auxin-responsive genes involved in developmental...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... modifications and factor recruitment at the murine b-globin locus in uninduced embryonic stem cells (day 0), as well as that of primitive and definitive erythroid cells (days and 12, respectively) Using ... transcription during in vitro erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells ... composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the Rex-1 and b-actin genes but not the embryonic and adult globin genes (Fig 2)...
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Food Security And Sustainable Development In Southern Africa pptx
... social development, women and habitat relating directly and indirectly to food security Within the UN system alone, including the international financial institutions, 14 Food Security and Sustainable ... African land policy as implemented in the period 1994 to 1999 Since joining the HSRC, he has been involved in research around integrated rural development including land reform, agricultural development, ... resolving food insecurity and the real achievements in increasing global food security, the gap between the aspiration of eradicating hunger and the continuing reality, portrayed, in this case, in...
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Press Freedom and Economic Development in Latin America 2008 docx
... author sustains that “the broad access to independent media is very closely related with systematic indicators of good governance and human development In this same line and using similar indicators ... freedom and economic development are strongly linked to each other Another interesting data that is revealed by the ranking of Press Freedom and Economic Development in Latin America is linked ... the process of installing modern democracies and the feelings and opinions that those aroused in Europe and the Western world is Alexis de Tocqueville (1805 – 1859) Being critical and distrustful...
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The Business of Women Female Enterprise and Urban Development in Northern England 1760–1830 ppt
... Middling women could be found operating freely in the French guild system,²³ trading independently in Edinburgh, Glasgow, and Geneva,²⁴ and acting as merchants in northern German and Scandinavian ... similar developments in the Midlands, see Andrew Hann, ‘Industrialisation and the Service Economy’ in Towns, Regions and Industries: Urban and Industrial Change, 1700–1840, ed Jon Stobart and Neil ... products in 1760 and 1770, and this provincial dominance was also evident in Leeds and Sheffield in 1800 Conversely, London appeared more prominent in all three towns at various points between 1810 and...
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KNOWLEDGE TRANSFER AND COMPETENCE DEVELOPMENT IN COMPLEX PAPER PRODUCTION ENVIRONMENTS pdf
... constructed concept and tools is verified in industrial case studies both in Finland and in China In more detail the contribution of the author is clarified in the following: Development of the ... transfer and competence development continue in 2002 in a paper machine start-up project in Finland The concept and tools were constructed and developed further based on the experiences gained in the ... including individual and organizational learning, in single and double loop and a systemic model in building learning organizations are reviewed 3.1 A resource-based view of a firm as an open system...
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Higher Education and Economic Development in Africa pot
... degree and diploma programs in fields relevant to economic development, including education, engineering, health and business Expand postgraduate training capacity – Ph.D & Masters programs Train ... Goal is to increase training to technical and vocational education institutions, especially in business education, and to partner with businesses There has been a rapid increase in developing new ... goals include improving working conditions and student living conditions, and strengthening the quality of higher education and research The latter includes strengthening tertiary education and...
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Economic Costs and Benefits of Allocating Forest Land for Industrial Tree Plantation Development in Indonesia pdf
... capture of foreign investment in the development of the related pulp industry, and increases in the production and exports of pulp and paper In fact, however, and when economic costs and benefits are ... quantity (of forest land) demanded (in 2003); S = supply (of forest land) curve Economic Costs and Benefits of Allocating Forest Land for Industrial Tree Plantation Development in Indonesia P D Before ... more forestland for conversion into HTI pulp plantations Economic Costs and Benefits of Allocating Forest Land for Industrial Tree Plantation Development in Indonesia INTRODUCTION Pulp industries...
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