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in vitro production of enucleated red blood cells from hematopoietic stem and progenitor cells

Báo cáo y học:

Báo cáo y học: "ene expression analysis of human red blood cells"

Y học thưởng thức

... presence of genes in human RBCs encoding initiation, activation and regulation of transcription and translation (for instance RNA polymerises I,II,III, zinc/PHD fingerDNA-binding proteins, cysteinyl, ... to understand the mechanisms and the biological meaning of these findings But gene expression profiling of human erythrocyte could be an important key for understanding the machinery of anucleate ... The finding of RNA in anucleate cells like erythrocytes support the idea of nucleus independent protein synthesis and supports data 20 about possible mechanism of globin m-RNA stability in human...
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Tài liệu Báo cáo khoa học: Glycomics-based analysis of chicken red blood cells provides insight into the selectivity of the viral agglutination assay docx

Tài liệu Báo cáo khoa học: Glycomics-based analysis of chicken red blood cells provides insight into the selectivity of the viral agglutination assay docx

Báo cáo khoa học

... analysis of cRBCs U Aich et al of RBCs occurs when the addition of a limiting amount of virus results in ‘crosslinking’ of RBCs through binding of multiple RBCs to HAs present on a single virus; ... for understanding the limitations of the assay and for better interpretation of the hemagglutination assay results In the present study, distinct analytical approaches including combining 2D-NMR, ... explorerÔ software TOF ⁄ TOF fragmentation spectra were recorded using negative-ion linear mode with standardized MS ⁄ MS acquisition and processing methods after tuning of the instrument TOF ⁄ TOF spectra...
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Báo cáo khoa học: V1–Varia V1-001P Hemolysis of human red blood cells by riboflavin-Cu(II) system: enhancement by azide. ppt

Báo cáo khoa học: V1–Varia V1-001P Hemolysis of human red blood cells by riboflavin-Cu(II) system: enhancement by azide. ppt

Báo cáo khoa học

... Bauhinia bauhinoides Cruzipain Inhibitor (BbCI) and Bauhinia bauhinioides Kallikrein Inhibitor (BbKI) respectively are cysteine and serine proteinase inhibitors homologous to plant Kunitz-type inhibitors, ... diffracting crystal of mouse serine racemase V1-048P The effect of antracyclines on histone proteins in active and inactive chromatin M Jafari1,2 and A Rabbani1 1Institute of Biochemistry and Biophysics, ... anthracycline antibiotics, which are widely used in treatment of variety of cancers The main target of these drugs is DNA in which inhibit DNA replication and transcription In chromatin, the binding of...
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Atomic force microscope imaging of human red blood cells and theri mechanical properties

Atomic force microscope imaging of human red blood cells and theri mechanical properties

Tổng hợp

... species of spectrin is the predominant form of spectrin in the RBC Hexamers and other oligomeric forms of spectrin have been observed in vitro and also may contribute to the structure of the membrane ... presence of protein 4.1 Protein 4.1 has a molecular weight of around 78,000 daltons, and is present in RBCs at about 200,000 copies per cell Protein 4.1 increases the binding of spectrin to actin by ... dehydrated and fresh samples of red blood cells to observe their general morphology and surface structure Tapping Mode in air, Contact Mode in air, and Contact Mode in fluid were used during the scanning...
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Báo cáo sinh học:

Báo cáo sinh học: "In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2" pot

Hóa học - Dầu khí

... immune cells resulting in tumor growth inhibition (reviewed in [25] and [1]) In mouse systems, ex vivo transduction of chemokines into tumor cells has provided potent tumor vaccines inducing tumor ... obtained from CRC cells on day of culture were tested for the presence of CCL2, CCL3, CCL15, CCL19, CCL21 and CXCL11 using ELISA kits (R&D Systems) Phenotyping Phenotyping of tumor cells and T cells ... fibroblasts and collagen Reconstructs were harvested on day (3 days after adding T cells) , fixed in buffered formalin and embedded in paraffin a, b: Staining with H&E c, d: Specific brown staining of...
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Báo cáo hóa học:

Báo cáo hóa học: " In vitro effects of selenium deficiency on West Nile virus replication and cytopathogenicity" ppt

Hóa học - Dầu khí

... achieved in both the Vero and SK-N-SH cell lines In vitro response of Vero and SK-N-SH cells to Se deficiency Figure In vitro response of Vero and SK-N-SH cells to Se deficiency Vero and SK-N-SH cells ... lines and/ or due to the method used to induce Se deficiency in our in vitro model In studies employing Jurkat E6 cells, the Se-deficient medium comprised of insulin, transferrin and bovine serum ... staining at 48 and 72 hr after infection, and expressed as percentage decline in infected cells grown in control, Se-, and Se+ media as compared to corresponding mockinfected cells *p < 0.05 and **p...
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báo cáo hóa học:

báo cáo hóa học:" In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2" pptx

Hóa học - Dầu khí

... immune cells resulting in tumor growth inhibition (reviewed in [25] and [1]) In mouse systems, ex vivo transduction of chemokines into tumor cells has provided potent tumor vaccines inducing tumor ... obtained from CRC cells on day of culture were tested for the presence of CCL2, CCL3, CCL15, CCL19, CCL21 and CXCL11 using ELISA kits (R&D Systems) Phenotyping Phenotyping of tumor cells and T cells ... fibroblasts and collagen Reconstructs were harvested on day (3 days after adding T cells) , fixed in buffered formalin and embedded in paraffin a, b: Staining with H&E c, d: Specific brown staining of...
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IMPACT OF ALCAM (CD166) ON HOMING OF HEMATOPOIETIC STEM AND PROGENITOR CELLS Mariya Aleksandrova

IMPACT OF ALCAM (CD166) ON HOMING OF HEMATOPOIETIC STEM AND PROGENITOR CELLS Mariya Aleksandrova

Y khoa - Dược

... ON HOMING OF HEMATOPOIETIC STEM AND PROGENITOR CELLS The potential of hematopoietic stem cells (HSC) to home and to anchor within the bone marrow (BM) microenvironment controls the ability of transplanted ... captured within the first 20 hours after injection of donor cells Transplanted stem and progenitor cells migrate to the BM to anchor within specialized niches and begin dividing, thus, repopulating ... mononuclear cells 22 In vitro quantitation of colony forming units (CFU) contained in phenotypically defined groups of BM-homed cells Speculations about cells from the osteoblastic lineage playing a...
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Báo cáo y học:

Báo cáo y học: "Gender differences and inflammation: an in vitro model of blood cells stimulation in prepubescent children" pptx

Báo cáo khoa học

... already reported the production of higher levels of cytokines by male's cells, ex vivo [13,14] in humans [15-18] and in animals [19-21] We have explored the capacity of whole blood cells to produce ... derived cytokine production [22] We have selected a direct stimulation with graded doses of LPS and Pokeweed Mitogen lectin as stimulants in vitro LPS-induced signalling in macrophages, and in other ... increased production of some cytokines in males compared with females This situation contrasts with numerous clinical observations in vivo of increased production of inflammatory markers in females The...
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Báo cáo y học:

Báo cáo y học: "Gender differences and inflammation: an in vitro model of blood cells stimulation in prepubescent children" pot

Báo cáo khoa học

... already reported the production of higher levels of cytokines by male's cells, ex vivo [13,14] in humans [15-18] and in animals [19-21] We have explored the capacity of whole blood cells to produce ... derived cytokine production [22] We have selected a direct stimulation with graded doses of LPS and Pokeweed Mitogen lectin as stimulants in vitro LPS-induced signalling in macrophages, and in other ... increased production of some cytokines in males compared with females This situation contrasts with numerous clinical observations in vivo of increased production of inflammatory markers in females The...
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Báo cáo khoa học:

Báo cáo khoa học: "Nucleated red blood cells in the blood of medical intensive care patients indicate increased mortality risk: a prospective cohort study" doc

Báo cáo khoa học

... Stachon A, Bolulu O, Holland-Letz T, Krieg M: Association between nucleated red blood cells in blood and the levels of erythropoietin, interleukin-3, interleukin-6, and interleukin12p70 Shock 2005, ... Incidence of nucleated red blood cells in the blood of hospitalised patients Infusion Ther Transfus Med 2001, 28:263-266 Stachon A, Sondermann N, Imöhl M, Krieg M: Nucleated red blood cells indicate ... The total in- hospital mortality of NRBC-positive patients of this Figure medical intensive care patients blood cells (NRBCs) in the blood of Concentration of nucleated red who have died from various...
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Báo cáo y học:

Báo cáo y học: " Age of red blood cells and mortality in the critically ill" ppsx

Báo cáo khoa học

... maximum age of red blood cells A locally weighted nonparametric smoother (LOWESS) for the predicted probability of death and the maximum age of red blood cells transfusion hemoglobin and higher ... confidence interval) according to the maximum age of red blood cells (RBCs) (days) Patients with the maximum age of RBCs exceeding each cut-off point are excluded Recently, Weinberg and colleagues ... received from the Australian Red Cross Blood Service, and in- kind support from the Australian and New Zealand Intensive Care Research Centre The present study is a collaboration of the Australian and...
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phosphatidylserine exposure in red blood cells: a suggestion for the active role of red blood cells in blood clot formation

phosphatidylserine exposure in red blood cells: a suggestion for the active role of red blood cells in blood clot formation

Tổng hợp

... Young and old red blood cells 23 2.5.2 Ca2+ content in young and old red blood cells 24 2.5.3 Influence of ageing on membrane redox system in red blood cells 25 2.5.4 Relevance of ... Scramblases in red blood cells 109 5.4 Phosphatidylserine exposure in red blood cells 111 5.5 Adhesion of red blood cells 118 5.6 Red blood cells in the process of thrombosis ... exposure of young and old red blood cells 101 4.5.6 Phosphatidylserine exposure of stored red blood cells 103 4.5.7 Membrane redox activity of young and old red blood cells 105 4.5.8...
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báo cáo hóa học:

báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx

Hóa học - Dầu khí

... HCC cells in vitro assay Methods Cell lines K562 cells (American Type Culture Collection) were maintained in DMEM medium Colorectal carcinoma cell lines (COLP-2 and COLM-6) were maintained in ... DCs/auto-HCC in vitro, the expression of IFN-γ in both CD4+ and CD8+ T cells were much lower (Figure 6D) In contrast, the expression of IFN-γ in CD4+ and CD8+ T cells significantly increased after vaccination ... factors derived from the HCC cells inhibit maturation of DCs and DCs/tumor fusion cells These fusion cells, in turn, promote the induction of CD4+ CD25high Foxp3+ Treg and impair induction of antigen-specific...
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Báo cáo sinh học:

Báo cáo sinh học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strain" pot

Hóa học - Dầu khí

... information concerning interactions between MYXV and bovine cells is available yet In this study, we characterized the infection of bovine cell lines and bovine peripheral blood mononuclear cells ... of viral maturation are impaired in BT cells A B To evaluate MYXV infection in blood primary bovine cells, peripheral blood mononuclear cells (PBMC) were infected Bovine blood was collected in ... comparing two different MYXV strains (a wild-type strain (T1) and a cell-cultured attenuated vaccinal strain (SG33) [14]) we verified the stability of the viral tropism in vitro Findings Three bovine...
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Báo cáo hóa học:

Báo cáo hóa học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains" potx

Hóa học - Dầu khí

... information concerning interactions between MYXV and bovine cells is available yet In this study, we characterized the infection of bovine cell lines and bovine peripheral blood mononuclear cells ... of viral maturation are impaired in BT cells A B To evaluate MYXV infection in blood primary bovine cells, peripheral blood mononuclear cells (PBMC) were infected Bovine blood was collected in ... comparing two different MYXV strains (a wild-type strain (T1) and a cell-cultured attenuated vaccinal strain (SG33) [14]) we verified the stability of the viral tropism in vitro Findings Three bovine...
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báo cáo hóa học:

báo cáo hóa học:" Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ ADAM17 gene expression during in vitro maturation of porcine COCs" doc

Hóa học - Dầu khí

... release of the EGF domain by TACE/ADAM17 acts on cumulus cells, which induce cumulus expansion In in vitro maturation of oocytes, COCs were recovered from antral follicles and cultured with FSH and/ or ... remained unclear during in vitro maturation of porcine COCs http://www.ovarianresearch.com/content/2/1/20 The binding of FSH and/ or LH in granulosa cells to its own receptors led to rapidly and ... example, prostagrandin E2 (PGE2) that produced from granulosa cells and cumulus cells by prostagradin synthase (PTGS2) was required for induction of Has2 and Tnfaip6 gene, cumulus expansion and oocyte...
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Báo cáo khoa học:

Báo cáo khoa học: "In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs" pdf

Báo cáo khoa học

... bovine serum (FBS, Sigma, Japan) and 1% antibiotics and antimicotics (Gibco, Japan), and they were maintained at 37°C in a humidified atmosphere of 5% CO2 in air U-CH1-N cells and cell doubling ... treatment, cycling cells in T12.5 culture flasks were exposed to series of concentration of bleocin, a single component of bleomycin family group A (Calbiochem, Japan), which induces DNA strand breaks, ... ionizing radiation or chemical treatment, cells were trypsinized and re-plated in P-100 cell culture dishes HeLa and U87-MG cells were cultured for 10 to 14 days, and U-CH1-N cells were kept in...
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báo cáo khoa học:

báo cáo khoa học: " In vitro behaviour of endothelial cells on a titanium surface" doc

Báo cáo khoa học

... binding to an ECM protein (e.g fibronectin), a number of integrins mediate cellular signaling and functions It was shown that α5β1 integrin, a receptor for both fibronectin (FN) and vitronectin ... are also considered to be the main receptors of ECM proteins, such as fibronectin, laminin, collagens, and vitronectin Altogether, these proteins constitute the main mediators of cell-ECM adhesion ... viability Indeed, the interaction of implants with host cells, and in particular with endothelial cells, might cause activation of adhesion molecules culminating with cytokine generation [2] In fact,...
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báo cáo khoa học:

báo cáo khoa học: " In vitro proliferation of human osteogenic cells in presence of different commercial bone substitute materials combined with enamel matrix derivatives" pdf

Báo cáo khoa học

... active EMD ingredients are ligands, such as amelogenin, ameloblastin, enamelin, and tuftelin, that play a crucial role in the development of teeth and supporting structures [18,19] A recent systematic ... within a scaffold [30-32] Furthermore, it is known that an increasing number of interconnective pores raises the internal surface area of a BSM, with promotion of the growth of regenerative cells ... after 24 h and d using the t-test Page of (page number not for citation purposes) Head & Face Medicine 2009, 5:23 Conclusion In our study, the addition of EMD resulted in an increase in AB reduction...
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