... thefinal manuscript.AcknowledgementsWe thank Robert Haynes for the countless hours spent in caring for and maintaining precise records of these RI lines and Sung Kim for his help in analyzing ... crosso-vers in Aec2 of chromosome 1, consisting of 33 lines with asingle crossover in Aec2 and 16 lines with a crossover in bothAec1 and Aec2. In addition, 2 lines were established usingmice ... num-bers of recombinant events, there do not appear to be anycrossover hotspots.Disease profiling of the recombinant inbred linesSjS-like disease in our NOD-derived mouse lines, includingC57BL/6.NOD-Aec1Aec2,...
... as of today, especially for a large number of DNA samples. The newly identified SNP were used for exclusion of the four investigated candidategenes in canine eye disease for a large number of ... results in fourcanine retina-specific genes by SSCP analysis and DNAsequencing in 29 breeds of dogs. In addition, we use iden-tified single nucleotide polymorphisms (SNP) for indirectexclusion of ... 5(page number not for citation purposes) In this study we investigated four retina-specific genes, ATP binding cassette transporter retina (ABCA4), con-nexin 36 (CX36), c-mer tyrosin kinase receptor...
... expression in itsflanking region. These findings clarify the crucial role of RIN in the transcriptional regulation of ripening initiationand progression.BackgroundRipening processes of many kinds of ... ces in the LeEXP1 andLeACS4 promotersaswellasanalyzingthepossibleCArG boxes.Binding of RIN to the CArG boxes in the ripening-inducedgene promoters in vivo and in vitroTo distinguish the genes ... encoding a proteinsimilar to thioredoxin y1. An increase in the transcript level of this gene was observed with ripening in normal fruitbut not in the rin mutant, suggesting that RIN possibly induces...
... set in green house in Faculty of Agronomy, Hanoi University for Agriculture, Vietnam during spring cropping season in 2006. 2.2. Planting Seed ofrice cultivars were incubated and sown in ... stomatal conductance contributed to droughttolerancein rice plant. The larger number of root per plant was the reason for better recovering ofrice plant after drought treatment. REFERENCES ... parameters: At three stages as before draining, during drought treatment and 4 days after recovering, 4 plants of each rice cultivars were randomly selected for measuring photosynthetic Pham Van...
... thediscriminatory peaks found in this study (Continued)Coronin, actinbinding protein, 1B55.7Keratin 9 62Tubulin, beta polypeptide 47.7Tubulin, alpha, ubiquitous 50.1Beta-actin 41.7Actin, gamma ... E:Diagnostic markers for diseases: SELDI-TOF profiling of pig sera for PRRS.Dev Biol 2008, 132:399–403.21. Zhong L, Taylor DL, Whittington RJ: Proteomic profiling of ovine serum bySELDI-TOF MS: optimisation, ... pig proteins identified in other studies and thediscriminatory peaks found in this study (Continued)Chaperonin containingTCP1, subunit 5(epsilon) protein59.6Chaperonin containingTCP1,...
... determining the suitability of 10 commonly usedreference genesin the context of infection with variousstrains of HIV-1, HSV-1, CMV and VZV in a range of bothprimary and cultured cell lines using ... reliablereference gene in HEP2 cells infected with the same strain of HSV-1). These findings therefore highlight the impor-tance of re-evaluating the choice housekeeping genes whenmaking even slight ... microtiter plates in a final volume of 25 μlunder the following cycle conditions: 50°C for 2 minutes,95°C for 2 minute, 45 cycles of 95°C for 15 seconds 55°C for 30 seconds and 72°C for 30 seconds.Data...
... determining the suitability of 10 commonly usedreference genesin the context of infection with variousstrains of HIV-1, HSV-1, CMV and VZV in a range of bothprimary and cultured cell lines using ... reliablereference gene in HEP2 cells infected with the same strain of HSV-1). These findings therefore highlight the impor-tance of re-evaluating the choice housekeeping genes whenmaking even slight ... microtiter plates in a final volume of 25 μlunder the following cycle conditions: 50°C for 2 minutes,95°C for 2 minute, 45 cycles of 95°C for 15 seconds 55°C for 30 seconds and 72°C for 30 seconds.Data...
... experimentaltransmission of infection and disease in inoculees, (ii)examination of seroprevalence rates in flocks with highand low PDD incidences, (iii) documentation of serocon-version accompanying development of ... since the first description of PDD. Although a viral etiology has long been suspected, aconvincing lead for a responsible viral pathogen has beenlacking. By combining veterinary clinical investigationwith ... kidneys, brain) were placed into RNAlaterwithin 1 minute of death and frozen within 2 minutes of death. Duplicate samples were collected for histopatho-logic diagnosis of PDD.Inclusion criteriaPDD-positive...
... a final concentration of 0.3% (v/v) and incubated at 37oC for 24 h with shaking. Sterility testing was performed by placing 0.5 ml of the killed vaccine into 25 ml of enrichment broth, incubated ... Results of mannose-resistant haemagglutination: The two ETEC strains were examined for mannose-resistant haemagglutinating activity using Sheep Red Blood Cells. Both strains were tested using overnight ... results of haemagglutination of cultures of each strain are presented in Table 1. Mannose-resistant haemagglutination was observed at 37oC, but not at 18oC for both strains, confirming the...
... signaling [18,19].PAK proteins constitute a family of serine/threonine kinasesthat utilizes the CRIB (Cdc42/Rac interactive binding)domain to bind to small GTPases; members of the familyinclude ... TRAC-1, for T-cell RING protein in activa-tion. As shown in Figure 4g, TRAC-1 has a RING fingerdomain, which is characteristically found in a class of pro-teins collectively called ubiquitin ligases ... protein-tyrosinekinases for ubiquitination and therefore to down-regulatetheir signaling [30]. The TRAC-1 hit we obtained has a trunca-tion in the carboxyl terminus but still retains the intact...
... list of thousands of genes, and it is not clear which genes are important for what.A key idea, originating from engineering science in generaland computer science in particular, is the notion of ... (GATA3) regulating one of this subset, the ITKgene, a promoter analysis was performed. The final module of 37 disease-associated genes consisted ofgenes listed in publicdatabases as having relevant ... ITK.Finally, using available databases, 47 genes were identifiedas interacting with GATA3. The expression data were used tofilter out 10 inactive genes, thus leaving a final module of 37 disease-associated...
... School of Medicine, Department of Medicine, IB424G, 975West Walnut Street, Indianapolis, IN 46202, USA.2Indiana University School of Medicine, Department of Psychiatry, PR116, Indianapolis, IN ... the introgressedinterval. The goal of this study was to identify candidategenes that may influence alcohol consumption bycomparing gene expression in five brain regions of alcoh ol-naïve inbred ... onsidered necessary for an ani-mal model of alcoholism [39,40]. Subsequently, inbredalcohol-preferring (iP) and -nonpreferring (iNP) strainswere established; these inbred strains maintain highlydivergent...
... information in expres-sion profiles [2]; applied linear transformations to mea-sure the discriminative information ofgenes [3]; andcombined information from multiple assessments [4].One of ... mutual influence of neighboring genes withina pathway, including the effects of genetic buffering.Because the TIF is c omputed for one pathway at a time,PWEA cannot detect genetic buffering exerted ... experimental findings. As would beexpected of any method designed to aid in the interpre-tation of expression data, the pathways reported in PWEA give insights into the nature of the differenttypes of...
... straightforward method for the identificationof host genes involved in viral infection. We succeeded in establishing a cDNA-AFLP system to help track changes in geneexpression patterns in N. ... these genes influence the accumulation of BaMV protein.Nine of the 15 genes showed either a positive or a negative influence on the accumulation of BaMV protein. Sixknockdown plants showed an increase ... accumulation of BaMV in plants. An interesting observation was that eight of the nine plants showing anincrease in BaMV coat protein were associated with cell rescue, defense, death, aging, signal...
... protein, a glycine rich proteinand a pectinesterase inhibitor-domain containing pro-tein), and one gene encoding a flavin-bin ding monooxy-genase-likefamilyproteinwhichhastheinferredfunction in ... genes detected in thisstudy are, though incomplete, important in determiningrhizome initiation and development in O. longistami-nata. Detailed examination of the functions of this set of genes ... in RT include those encodingtwo CEN-like proteins, two meiosis 5 proteins, two GAresponse proteins, and two auxin-responsive proteins.Also, the expression levels of two meiosis 5 proteingenes...